Patents by Inventor Hiromi Takenaka
Hiromi Takenaka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9557339Abstract: The present invention is to provide a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the present invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 ?M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the present invention includes the binder for a rodent-derived IgG antibody of the present invention. The detection kit for detecting a rodent-derived IgG antibody of the present invention includes the detection reagent for detecting a rodent-derived IgG antibody of the present invention.Type: GrantFiled: July 21, 2014Date of Patent: January 31, 2017Assignee: NEC Solution Innovators, Ltd.Inventors: Hiromi Takenaka, Yoshihito Yoshida, Katsunori Horii, Makio Furuichi, Hirotaka Yagi, Jou Akitomi, Mineko Yamaguchi, Shintarou Katou, Kensaku Nishikata, Iwao Waga
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Patent number: 9278108Abstract: A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10?7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.Type: GrantFiled: July 16, 2010Date of Patent: March 8, 2016Assignees: NEC Solution Innovators, Ltd., Kanagawa Prefectural Hospital OrganizationInventors: Hiromi Takenaka, Jou Akitomi, Shintarou Katou, Shotaro Tsuji, Takashi Ohtsu, Iwao Waga
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Publication number: 20140370618Abstract: The present invention is to provide a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the present invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 ?M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the present invention includes the binder for a rodent-derived IgG antibody of the present invention. The detection kit for detecting a rodent-derived IgG antibody of the present invention includes the detection reagent for detecting a rodent-derived IgG antibody of the present invention.Type: ApplicationFiled: July 21, 2014Publication date: December 18, 2014Applicant: NEC SOFT, LTD.Inventors: Hiromi TAKENAKA, Yoshihito YOSHIDA, Katsunori HORII, Makio FURUICHI, Hirotaka YAGI, Jou AKITOMI, Mineko YAMAGUCHI, Shintarou KATOU, Kensaku NISHIKATA, Iwao WAGA
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Patent number: 8852954Abstract: The invention provides a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 ?M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the invention includes the binder for a rodent-derived IgG antibody of the invention. The detection kit for detecting a rodent-derived IgG antibody of the invention includes the detection reagent for detecting a rodent-derived IgG antibody of the invention.Type: GrantFiled: August 21, 2009Date of Patent: October 7, 2014Assignee: NEC Solution Innovators, Ltd.Inventors: Hiromi Takenaka, Yoshihito Yoshida, Katsunori Horii, Makio Furuichi, Hirotaka Yagi, Jou Akitomi, Mineko Yamaguchi, Shintarou Katou, Kensaku Nishikata, Iwao Waga
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Publication number: 20130022967Abstract: The invention provides a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 ?M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the invention includes the binder for a rodent-derived IgG antibody of the invention. The detection kit for detecting a rodent-derived IgG antibody of the invention includes the detection reagent for detecting a rodent-derived IgG antibody of the invention.Type: ApplicationFiled: August 21, 2009Publication date: January 24, 2013Applicant: NEC SOFT, LTD.Inventors: Hiromi Takenaka, Yoshihito Yoshida, Katsunori Horii, Makio Furuichi, Hirotaka Yagi, Jou Akitomi, Mineko Yamaguchi, Shintarou Katou, Kensaku Nishikata, Iwao Waga
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Patent number: 8278120Abstract: The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an ? helix-like secondary structure: PFLLSHCMGYGFYHF (?1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.Type: GrantFiled: January 25, 2007Date of Patent: October 2, 2012Assignee: NEC Soft, Ltd.Inventors: Kyoko Suto, Hiromi Takenaka, Yasuhiro Takenaka
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Publication number: 20120208867Abstract: A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10?7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.Type: ApplicationFiled: July 16, 2010Publication date: August 16, 2012Inventors: Hiromi Takenaka, Jou Akitomi, Shintarou Katou, Shotaru Tsuji, Takashi Ohtsu, Iwao Waga
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Patent number: 8203032Abstract: The present invention provides a process for generation of a transformed plant capable of emitting fluorescence by introducing a gene encoding a non-plant-derived fluorescent protein into a plant such that the fluorescent protein is recombinantly expressed in the active form of its mature protein in the leaf or petal of the plant, and also provides a transformed garden plant capable of emitting fluorescence that is generated by using the process. For example, cDNA encoding the full-length amino acid sequence of a Chiridius poppei-derived fluorescent protein CpYGFP or its H52F modified protein CpYGFP H52F is inserted into a T-DNA-based expression vector system, which is in turn introduced into the chromosomal DNA of a plant. As a result, the transformed plant thus generated can exhibit fluorescence attributed to these fluorescent proteins and exhibit no substantial difference in the other phenotypes from wild-type one of the plant.Type: GrantFiled: July 25, 2007Date of Patent: June 19, 2012Assignee: NEC Soft, Ltd.Inventors: Iwao Waga, Hiromi Takenaka, Shu Muto
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Patent number: 8043850Abstract: The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO: 1 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.Type: GrantFiled: September 4, 2008Date of Patent: October 25, 2011Assignees: NEC Soft, Ltd., National Institute of Agrobiochemical ScienceInventors: Frederick I. Tsuji, Hiroshi Mizuno, Kenji Takase, Mitsuru Momma, Zui Fujimoto, Toshiyuki Wako, Yasuhiro Takenaka, Noboru Nakura, Hiromi Takenaka
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Patent number: 7871803Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: GrantFiled: December 9, 2004Date of Patent: January 18, 2011Assignee: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20100043104Abstract: The present invention provides a process for generation of a transformed plant capable of emitting fluorescence by introducing a gene encoding a non-plant-derived fluorescent protein into a plant such that the fluorescent protein is recombinantly expressed in the active form of its mature protein in the leaf or petal of the plant, and also provides a transformed garden plant capable of emitting fluorescence that is generated by using the process. For example, cDNA encoding the full-length amino acid sequence of a Chiridius poppei-derived fluorescent protein CpYGFP or its H52F modified protein CpYGFP H52F is inserted into a T-DNA-based expression vector system, which is in turn introduced into the chromosomal DNA of a plant. As a result, the transformed plant thus generated can exhibit fluorescence attributed to these fluorescent proteins and exhibit no substantial difference in the other phenotypes from wild-type one of the plant.Type: ApplicationFiled: July 25, 2007Publication date: February 18, 2010Applicant: NEC SOFT, LTD.Inventors: Iwao Waga, Hiromi Takenaka, Shu Muto
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Publication number: 20090318673Abstract: The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an ? helix-like secondary structure: PFLLSHCMGYGFYHF (?1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.Type: ApplicationFiled: January 25, 2007Publication date: December 24, 2009Applicant: NEC SOFT, LTD.Inventors: Kyoko Suto, Hiromi Takenaka, Yasuhiro Takenaka
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Publication number: 20090233320Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: ApplicationFiled: December 9, 2004Publication date: September 17, 2009Applicant: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20090123969Abstract: The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO: 1 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.Type: ApplicationFiled: September 4, 2008Publication date: May 14, 2009Applicants: NEC SOFT LTD., NATIONAL INSTITUTE OF AGROBIOCHEMICAL SCIENCEInventors: Frederick I. Tsuji, Hiroshi Mizuno, Kenji Takase, Mitsuru Momma, Zui Fujimoto, Toshiyuki Wako, Yasuhiro Takenaka, Noboru Nakura, Hiromi Takenaka
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Patent number: 7442768Abstract: The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO:1and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.Type: GrantFiled: September 30, 2004Date of Patent: October 28, 2008Assignees: NEC Soft, Ltd., National Institute of Agrobiochemical ScienceInventors: Frederick I. Tsuji, Hiroshi Mizuno, Kenji Takase, Mitsuru Momma, Zui Fujimoto, Toshiyuki Wako, Yasuhiro Takenaka, Noboru Nakura, Hiromi Takenaka
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Publication number: 20050221338Abstract: The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence: MTTFKIESRI HGNLNGEKFE LVGGGVGEEG RLEIEMKTKD KPLAFSPFLL SHCMGYGFYH 60 FASFPKGTKN IYLHAATNGG YTNTRKEIYE DGGILEVNFR YTYEFNKIIG DVECIGHGFP 120 SQSPIFKDTI VKSCPTVDLM LPMSGNIIAS SYARAFQLKD GSFYTAEVKN NIDFKNPIHE 180 SFSKSGPMFT HRRVEETHTK ENLAMVEYQQ VFNSAPRDM 219 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.Type: ApplicationFiled: September 30, 2004Publication date: October 6, 2005Inventors: Frederick Tsuji, Hiroshi Mizuno, Kenji Takase, Mitsuru Momma, Zui Fujimoto, Toshiyuki Wako, Yasuhiro Takenaka, Noboru Nakura, Hiromi Takenaka
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Publication number: 20050054838Abstract: Isoforms of apoaequorin and isoforms of aequorin are isolated and purified from recombinant apoaequorin and a solution containing regenerated aequorin, respectively, by gradient elution chromatography. As a result, aequorin can be isolated and purified.Type: ApplicationFiled: July 22, 2004Publication date: March 10, 2005Inventors: Motohiro Otsuka, Hiroshi Mizuno, Frederic Tsuji, Hiromi Takenaka