Abstract: An object of the present invention is to provide a method by which a peptide having a specific binding capability that can be used for purification of a target molecule can be produced at a low cost, and specifically relates to a peptide fusion protein including one or more peptides having specific binding capability and a scaffold protein, the peptide being inserted into the amino acid sequence of the scaffold protein directly or via a peptide linker, and/or being linked to the N-terminal and/or C-terminal of the scaffold protein.

Abstract: Provided is novel porous cellulose having functionality that is not imparted to porous cellulose composed of unsubstituted cellulose, and a method for producing the same. Porous cellulose containing: unsubstituted cellulose; and a glucose unit-containing polymer excluding unsubstituted cellulose, wherein a content of the polymer is not more than 20 mass % in 100 mass % of a total of the polymer and the unsubstituted cellulose.

Abstract: Provided are porous cellulose particles containing chitosan and having predetermined pores on a surface and a predetermined particle diameter, and a method for producing the porous cellulose particles. Porous cellulose particles including unsubstituted cellulose and chitosan, wherein a content of the chitosan is not greater than 20 mass % in a total of 100 mass % of the unsubstituted cellulose and the chitosan, pores having a diameter from 0.05 to 5 ?m are observed in an image of surfaces of the porous cellulose particles observed by a scanning electron microscope, and a proportion of porous cellulose particles having a particle diameter from 10 to 200 ?m is not less than 90 mass %.

Abstract: Provided is a novel porous cellulose medium that can efficiently separate a large target molecule in a calibration standard. A porous cellulose medium including a porous cellulose particle having a particle size from 1 to 600 ?m, wherein, in sieving the porous cellulose medium for classification and using a fraction corresponding to aperture openings between 53 ?m and 106 ?m as a support for size exclusion chromatography, a polyethylene oxide standard is run through size exclusion chromatography with pure water as a mobile phase, and a weight average molecular weight Mw and a gel partition coefficient Kav of the polyethylene oxide standard satisfy Relationships (A) and (B) above: in a case where 4.80?log Mw?5.50,Kav>?0.445×log Mw+2.55??(A) in a case where 5.75?log Mw,0?Kav<0.19??(B).

Abstract: Provided is a separating agent that does not have a significantly reduced dynamic binding capacity (DBC) to a target substance even when the separating agent is continually CIP-treated under alkaline pH conditions. The separating agent includes a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and a lysine residue in the protein are bound by a chemical bond.

Abstract: Provided is a novel method for producing cellulose beads, the method being capable of suitably producing spherical cellulose beads. The method for producing cellulose beads includes: (Step 1) preparing a coagulating bath having a first liquid medium phase and a second liquid medium phase; (Step 2) forming a particulate cellulose solution in the first liquid medium phase by supplying a cellulose solution to the first liquid medium phase of the coagulating bath; and (Step 3) coagulating the particulate cellulose solution by supplying the particulate cellulose solution in the first liquid medium phase to the second liquid medium phase.

Abstract: An object of the present invention is to provide a method by which a peptide having a specific binding capability that can be used for purification of a target molecule can be produced at a low cost, and specifically relates to a peptide fusion protein including one or more peptides having specific binding capability and a scaffold protein, the peptide being inserted into the amino acid sequence of the scaffold protein directly or via a peptide linker, and/or being linked to the N-terminal and/or C-terminal of the scaffold protein.

Abstract: Provided is a separating agent with an improved dynamic binding capacity (DBC) to a target substance. The separating agent includes a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and added lysine residues in the protein are bound by chemical bonds.