Patents by Inventor Hiroshi Kawaminami
Hiroshi Kawaminami has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11852630Abstract: An immunochromatographic test piece is provided containing a control line detection reagent that is not affected by the concentration of an analyte to be detected. The immunochromatographic test piece is for quantifying an analyte in a biological sample, and includes (a) a conjugation pad impregnated with a detection reagent that specifically binds to the analyte, and a control line detection reagent; and (b) a porous membrane pad on which an antibody for capturing the analyte is immobilized upstream, and an antibody for capturing specifically the control line detection reagent is immobilized downstream.Type: GrantFiled: October 13, 2016Date of Patent: December 26, 2023Assignee: TOYOBO CO., LTD.Inventors: Hiroshi Kawaminami, Yuji Tsuji, Keizo Yoneda
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Publication number: 20180299455Abstract: [Problem] To provide an immunochromatographic test piece containing a control line detection reagent that is not affected by the concentration of an analyte to be detected. [Means for Solution] An immunochromatographic test piece for quantifying an analyte in a biological sample, the immunochromatographic test piece comprising (a) a conjugation pad impregnated with a detection reagent that specifically binds to the analyte, and a control line detection reagent; and (b) a porous membrane pad on which an antibody for capturing the analyte is immobilized upstream, and an antibody for capturing specifically the control line detection reagent is immobilized downstream.Type: ApplicationFiled: October 13, 2016Publication date: October 18, 2018Applicant: TOYOBO CO., LTD.Inventors: Hiroshi Kawaminami, Yuji Tsuji, Keizo Yoneda
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Patent number: 9487758Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: February 10, 2014Date of Patent: November 8, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9404144Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: GrantFiled: February 7, 2013Date of Patent: August 2, 2016Assignee: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9260699Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: April 30, 2014Date of Patent: February 16, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20150031059Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: ApplicationFiled: February 7, 2013Publication date: January 29, 2015Applicant: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20140287478Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: April 30, 2014Publication date: September 25, 2014Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI
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Publication number: 20140154777Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: February 10, 2014Publication date: June 5, 2014Applicant: TOYOBO CO., LTD.Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI
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Patent number: 8247189Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.Type: GrantFiled: March 15, 2012Date of Patent: August 21, 2012Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Masao Kitabayashi, Yoshiaki Nishiya
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Publication number: 20120171708Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent. A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence that has a 60% homology therewith, and that has an improved temperature dependency.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Patent number: 8039248Abstract: The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sample.Type: GrantFiled: August 25, 2009Date of Patent: October 18, 2011Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Yuji Tsuji, Masao Kitabayashi, Yoshiaki Nishiya
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Patent number: 7871805Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.Type: GrantFiled: January 9, 2009Date of Patent: January 18, 2011Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Aiba, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 7741100Abstract: The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.Type: GrantFiled: March 28, 2007Date of Patent: June 22, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Yuji Tsuji, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 7662600Abstract: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.Type: GrantFiled: November 13, 2007Date of Patent: February 16, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Yuji Tsuji, Masao Kitabayashi, Yoshiaki Nishiya
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Publication number: 20090317848Abstract: The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sampleType: ApplicationFiled: August 25, 2009Publication date: December 24, 2009Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Yuji TSUJI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Publication number: 20090155848Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.Type: ApplicationFiled: January 9, 2009Publication date: June 18, 2009Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi AIBA, Hiroshi KAWAMINAMI, Takahide KISHIMOTO, Yoshiaki NISHIYA
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Patent number: 7494794Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.Type: GrantFiled: March 30, 2007Date of Patent: February 24, 2009Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Aiba, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Publication number: 20080220460Abstract: Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.Type: ApplicationFiled: November 13, 2007Publication date: September 11, 2008Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Yuji TSUJI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Publication number: 20080020426Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.Type: ApplicationFiled: March 30, 2007Publication date: January 24, 2008Applicant: Toyo Boseki Kabushiki KaishaInventors: Hiroshi AIBA, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Publication number: 20080014611Abstract: The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.Type: ApplicationFiled: March 28, 2007Publication date: January 17, 2008Applicant: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Yuji Tsuji, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya