Abstract: Provided is a method for inducing T cells for a cell-based immunotherapy, comprising the steps of: (1) providing human pluripotent stem cells bearing genes encoding a T cell receptor specific for a desired antigen, and (2) inducing T cells from the pluripotent stem cells of step (1). The human pluripotent stem cells bearing genes encoding the desired antigen specific T cell receptor may be prepared by introducing genes encoding the desired antigen specific T cell receptor into pluripotent stem cells.

Abstract: Provided is a method for inducing T cells for a cell-based immunotherapy, comprising the steps of: (1) providing Rag 1 and/or Rag 2 gene knockout human pluripotent stem cells bearing genes encoding a T cell receptor specific for a desired antigen, and (2) inducing T cells from the pluripotent stem cells of step (1). Further provided are a cell-based immunotherapy method that uses the T cells for the cell-based immunotherapy and an iPS cell bank for the cell-based immunotherapy.

Abstract: Provided is a method for inducing T cells for a cell-based immunotherapy, comprising the steps of: (1) providing human pluripotent stem cells bearing genes encoding a T cell receptor specific for a desired antigen, and (2) inducing T cells from the pluripotent stem cells of step (1). The human pluripotent stem cells bearing genes encoding the desired antigen specific T cell receptor may be prepared by introducing genes encoding the desired antigen specific T cell receptor into pluripotent stem cells.

Abstract: Provided is a method for producing effector cells expressing a desired specificity determining region. In this method, material cells that have a cassette deck structure with a cassette tape gene comprising a gene encoding a marker protein in their genome, wherein the material cells can be differentiated into the effector cells, and wherein the marker protein can be expressed in the effector cells or progenitor cells thereof when the material cells are differentiated into the effector cells or progenitor cells thereof are provided first. The material cells are proliferated, the proliferated cells are differentiated into the effector cells, and then, the gene encoding the marker protein in the effector cells is exchanged with a gene encoding a protein that contribute the desired specificity.

Abstract: An object of the present invention is to provide a method for efficiently obtaining a lactic acid bacterium that is made to contain a large amount of double-stranded RNA; and a lactic acid bacterium having a high double-stranded RNA content obtained by the method. The object is achieved by: (1) a method for producing a double-stranded RNA-containing lactic acid bacterium, including a step of culturing a lactic acid bacterium under at least one condition of an aeration condition and a low-temperature condition lower than an optimum temperature, thereby obtaining the double-stranded RNA-containing lactic acid bacterium; (2) a double-stranded RNA-containing lactic acid bacterium, in which the content of double-stranded RNA is 2.0 times or more as compared with the content of double-stranded RNA when a bacterium of the same strain is cultured for the same culture time under an optimum temperature and non-aeration condition; or the like.

Abstract: The present invention relates generally to a population of stem cells (e.g., iPSCs or HSCs) that comprise nucleic acids encoding a T cell receptor and a chimeric antigen receptor directed to multiple distinct antigenic determinants, for example two distinct tumour antigenic determinants. The present invention is also directed to a population of T cells that co-express a T cell receptor and a chimeric antigen receptor directed to multiple distinct antigenic determinants, such as two distinct tumour antigenic determinants. The cells of the present invention can be derived from chosen donors whose HLA type is compatible with significant sectors of the populations, and are useful in a wide variety of applications, in particular in the context of the therapeutic treatment of neoplastic conditions.

Abstract: Provided is a method for inducing CD4?CD8+ T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4?CD8? T cells and CD4+CD8+ T cells, (2) removing CD4?CD8? cells from the cell culture obtained in step (1), and (3) differentiating the CD4+CD8+ cells in the cell culture into CD4?CD8+ T cells.

Abstract: The present invention relates generally to a population of stem cells (e.g., iPSCs or HSCs) that comprise nucleic acids encoding a T cell receptor and a chimeric antigen receptor directed to multiple distinct antigenic determinants, for example two distinct tumour antigenic determinants. The present invention is also directed to a population of T cells that co-express a T cell receptor and a chimeric antigen receptor directed to multiple distinct antigenic determinants, such as two distinct tumour antigenic determinants. The cells of the present invention can be derived from chosen donors whose HLA type is compatible with significant sectors of the populations, and are useful in a wide variety of applications, in particular in the context of the therapeutic treatment of neoplastic conditions.

Abstract: Provided is a method for inducing CD4?CD8+ T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4?CD8? T cells and CD4+CD8+ T cells, (2) removing CD4?CD8? cells from the cell culture obtained in step (1), and (3) differentiating the CD4+CD8+ cells in the cell culture into CD4?CD8+ T cells.

Abstract: Provided is a method for producing a cell incorporating an antigen specific receptor gene, which comprises the step of introducing an exogenous TCR or CAR gene into a material cell so that the introduced gene is expressed under the T cell receptor expression control system of the material cell.

Abstract: The purpose of the present invention is to provide a method for producing a pipe material and to provide a mandrel with which resistance between a member to be processed and the mandrel during bending processing can be reduced and overall processing time can be shortened. The method for producing a pipe material includes: a step for inserting a mandrel, which is provided on the inside thereof with a flow path through which dry ice powder flows and spray holes at the tip thereof for spraying the dry ice powder, inside pipe material; a step for spraying the dry ice powder from the spray holes inside the pipe material; and a step for performing bending processing on the pipe material wherein the mandrel has been inserted.

Abstract: A medium transport device includes a medium guide member, a driving source, a first movement member, a movement restricting member, and a second moving member. The medium guide member is movable between a first guide position, in which the medium guide member guides a medium toward a receiving member on which the medium is loaded, and a second guide position, in which the medium guide member guides the medium from the receiving member into the device. The driving source moves the medium guide member. The first movement member moves the medium guide member in accordance with an operation of the driving source. The movement restricting member is movable together with the medium guide member.

Abstract: A medium transport device includes a transporting member, a medium offsetting member, a guide member, and an interlocking member. The guide member is located upstream of the transporting member in a medium transport direction. The guide member is movable between a first position, in which the guide member guides the medium toward the medium accommodating member, and a second position, in which the guide member guides the medium to a destination different from the medium accommodating member, to guide the medium. The interlocking member moves the guide member between the first position and the second position in conjunction with a movement of the medium offsetting member in a width direction of the medium offsetting member.

Abstract: A medium transport device includes a transporting member that transports a medium toward a medium accommodating member; a switching member that switches between a first guide position, in which the switching member guides the medium to be transported to the medium accommodating member, and a second guide position, in which the switching member guides the medium to be transported to a position different from the medium accommodating member, the switching member being movable between a first shift position and a second shift position in a width direction of the medium; and a movement member that moves the switching member to the first guide position to move the switching member to the first shift position, and that moves the switching member to the second guide position to move the switching member to the second shift position.

Abstract: Provided is a method for producing regulatory T cells for inducing immune tolerance in a subject. The method comprises the step of co-culturing regulatory T cells obtained from the subject with dendritic cells derived from iPS cells.

Abstract: An object of the present invention is to provide a method for efficiently obtaining a lactic bacterium having a high double-stranded RNA content obtained by the method. The object is achieved by: (1) a method for producing a double-stranded RNA-containing lactic acid bacterium, including a step of culturing a lactic acid bacterium under at least one condition of an aeration condition and a low-temperature condition lower than an optimum temperature, thereby obtaining the double-stranded RNA-containing lactic acid bacterium; (2) a double-stranded RNA-containing lactic acid bacterium, in which the content of double-stranded RNA is 2.0 times or more as compared with the content of double stranded RNA when a bacterium of the same strain is cultured for the same culture time under an optimum temperature and non-aeration condition; or the like.

Abstract: A medium transport device includes a transporting member, a medium offsetting member, a guide member, and an interlocking member. The transporting member transports a medium toward a medium accommodating member. The medium offsetting member moves the transporting member in a medium width direction to transport the medium to the medium accommodating member at positions shifted in the medium width direction. The guide member is located upstream of the transporting member in a medium transport direction. The guide member is movable between a first position, in which the guide member guides the medium toward the medium accommodating member, and a second position, in which the guide member guides the medium to a destination different from the medium accommodating member, to guide the medium. The interlocking member moves the guide member between the first position and the second position in conjunction with a movement of the medium offsetting member in a width direction of the medium offsetting member.

Abstract: A medium transport device includes a transporting member that transports a medium toward a medium accommodating member; a switching member that switches between a first guide position, in which the switching member guides the medium to be transported to the medium accommodating member, and a second guide position, in which the switching member guides the medium to be transported to a position different from the medium accommodating member, the switching member being movable between a first shift position and a second shift position in a width direction of the medium; and a movement member that moves the switching member to the first guide position to move the switching member to the first shift position, and that moves the switching member to the second guide position to move the switching member to the second shift position.

Abstract: A medium transport device includes a medium guide member, a driving source, a first movement member, a movement restricting member, and a second moving member. The medium guide member is movable between a first guide position, in which the medium guide member guides a medium toward a receiving member on which the medium is loaded, and a second guide position, in which the medium guide member guides the medium from the receiving member into the device. The driving source moves the medium guide member. The first movement member moves the medium guide member in accordance with an operation of the driving source. The movement restricting member is movable together with the medium guide member.

Abstract: Provided is a method for inducing CD4?CD8+ T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4?CD8? T cells and CD4+CD8+ T cells, (2) removing CD4?CD8? cells from the cell culture obtained in step (1), and (3) differentiating the CD4+CD8+ cells in the cell culture into CD4?CD8+ T cells.