Patents by Inventor Hiroshi Mizokami

Hiroshi Mizokami has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8647634
    Abstract: A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and collecting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
    Type: Grant
    Filed: December 24, 2009
    Date of Patent: February 11, 2014
    Assignee: The Chemo-Sero-Therapeutic Research Institute
    Inventors: Ryuichi Sakamoto, Susumu Baba, Masashi Sakaguchi, Hiroshi Mizokami
  • Publication number: 20130183330
    Abstract: A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
    Type: Application
    Filed: September 14, 2012
    Publication date: July 18, 2013
    Applicant: THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE
    Inventors: Ryuichi SAKAMOTO, Susumu Baba, Masashi Sakaguchi, Hiroshi Mizokami
  • Patent number: 8332159
    Abstract: A method of enhancing the efficacy of a monoclonal antibody preparation wherein antigens from patients are tested for their reactivity with the antibody. An amino acid sequence of an expressed protein is deuced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in a biopsy from a patient. This is compared with the previously determined amino acid sequence recognized by the monoclonal antibody preparation in order to assess the fitness of patients for administration of the monoclonal antibody preparation.
    Type: Grant
    Filed: February 18, 2004
    Date of Patent: December 11, 2012
    Assignee: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
    Inventors: Toshio Murakami, Hirofumi Higuchi, Keiichi Makizumi, Toshihiro Maeda, Hiroshi Mizokami
  • Publication number: 20120003257
    Abstract: A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
    Type: Application
    Filed: December 24, 2009
    Publication date: January 5, 2012
    Applicant: The Chemo-Sero-Therapeutic Research Institute
    Inventors: Ryuichi Sakamoto, Susumu Baba, Masashi Sakaguchi, Hiroshi Mizokami
  • Patent number: 7667009
    Abstract: A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided.
    Type: Grant
    Filed: April 14, 2004
    Date of Patent: February 23, 2010
    Assignee: Juridical Foundation the Chemo-Sero-Therapeutic Research Institute
    Inventors: Satoshi Koyanagi, Kenjiro Kawatsu, Toshio Murakami, Yoshinobu Miyatsu, Toshihiro Maeda, Hiroshi Mizokami
  • Patent number: 7531631
    Abstract: The present invention herein discloses a method for purifying human serum albumin, which is characterized in that it comprises the steps of heat-treating a human serum albumin-containing solution including impurities originated from a host cell, in the presence of a divalent cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron ion and zinc ion to thus selectively allow the impurities to undergo agglutination. The present invention also provides the highly purified human serum albumin prepared by the foregoing method.
    Type: Grant
    Filed: July 20, 2006
    Date of Patent: May 12, 2009
    Assignee: Juridical Foundation the Chemoserotherapeutic Research Institute
    Inventors: Shinichi Shibata, Kazuyuki Nakashima, Tetsurou Tanabe, Yoshinobu Miyatsu, Hiroshi Mizokami
  • Publication number: 20080113424
    Abstract: A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided.
    Type: Application
    Filed: April 14, 2004
    Publication date: May 15, 2008
    Applicant: Juridicical Foundation the Chemo-Sero-Therapeutic Research Institute
    Inventors: Satoshi Koyanagi, Kenjiro Kawatsu, Toshio Murakami, Yoshinobu Miyatsu, Toshihiro Maeda, Hiroshi Mizokami
  • Publication number: 20070065906
    Abstract: A heterologous protein free from an inducer and a method for producing said protein are provided. A method for producing a heterologous protein which comprises the step of optionally culturing at low temperature recombinant E. coli cells expressing a heterologous protein under control of a promoter capable of inducing expression through temperature shift and then culturing at high temperature said recombinant E. coli cells in the absence of an inducer to thereby allow for expression of said heterologous protein, or the step of culturing at high temperature said recombinant E. coli cells to thereby simultaneously allow for both cell proliferation and expression of said heterologous protein, and the heterologous protein obtained by said method that is free from an inducer. Such heterologous protein may include a major mite allergen, a secretary macrophage toxin from Actinobacillus pleuropneumoniae and a surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae.
    Type: Application
    Filed: February 20, 2004
    Publication date: March 22, 2007
    Applicant: JURIDICAL FOUNDATION the CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE
    Inventors: Satoshi Koyanagi, Keishin Sugawara, Yoshinobu Miyatsu, Toshio Murakami, Toshihiro Maeda, Hiroshi Mizokami
  • Publication number: 20060280751
    Abstract: A method for enhancing efficacy of a monoclonal antibody preparation is provided wherein antigens from patients are tested for their reactivity with said antibody. In accordance with the method of the invention, an amino acid sequence of an expressed protein is deduced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in biopsy from patients and is compared with the previously determined amino acid sequence recognized by said monoclonal antibody preparation to thereby assess fitness of patients for administration of said monoclonal antibody preparation.
    Type: Application
    Filed: February 18, 2004
    Publication date: December 14, 2006
    Inventors: Toshio Murakami, Hirofumi Higuchi, Keiichi Makizumi, Toshihiro Maeda, Hiroshi Mizokami
  • Publication number: 20060258850
    Abstract: The present invention herein discloses a method for purifying human serum albumin, which is characterized in that it comprises the steps of heat-treating a human serum albumin-containing solution including impurities originated from a host cell, in the presence of a divalent cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron ion and zinc ion to thus selectively allow the impurities to undergo agglutination. The present invention also provides the highly purified human serum albumin prepared by the foregoing method.
    Type: Application
    Filed: July 20, 2006
    Publication date: November 16, 2006
    Inventors: Shinichi Shibata, Kazuyuki Nakashima, Tetsurou Tanabe, Yoshinobu Miyatsu, Hiroshi Mizokami
  • Patent number: 7001885
    Abstract: Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.
    Type: Grant
    Filed: June 20, 2002
    Date of Patent: February 21, 2006
    Assignee: Juridical Foundation the Chemo-Sero-Therapeutic Research Institute
    Inventors: Satoshi Adachi, Hiroshi Mizokami, Yoshitaka Tajima, Yoshinobu Miyatsu, Toshinobu Nouchi, Yoshitaka Ushio
  • Patent number: 6908749
    Abstract: A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.
    Type: Grant
    Filed: June 20, 2002
    Date of Patent: June 21, 2005
    Assignee: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
    Inventors: Toshinobu Nouchi, Hiroshi Mizokami, Yoshitaka Tajima, Yoshinobu Miyatsu, Masahiro Sakaguchi, Kazunari Yagi
  • Patent number: 6869934
    Abstract: The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
    Type: Grant
    Filed: July 18, 2001
    Date of Patent: March 22, 2005
    Assignees: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute, Kowa Company, Ltd.
    Inventors: Hiroshi Mizokami, Shinichi Furukawa, Keishin Sugawara, Tatsufumi Onchi, Kazuhiro Komatsu, Satoshi Koyanagi, Hideo Yoshizaki
  • Publication number: 20050059805
    Abstract: The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
    Type: Application
    Filed: September 21, 2004
    Publication date: March 17, 2005
    Inventors: Hiroshi Mizokami, Shinichi Furukawa, Keishin Sugawara, Tatsufumi Onchi, Kazuhiro Komatsu, Satoshi Koyanagi, Hideo Yoshizaki
  • Publication number: 20030027991
    Abstract: A method for converting a multimer of human serum albumin into monomers thereof comprises the step of treating the multimer with an alkaline solution. This method permits the efficient and quite simple conversion of human serum albumin multimers into monomers thereof at a low cost.
    Type: Application
    Filed: June 21, 2002
    Publication date: February 6, 2003
    Applicant: Juridical Fdn. The Chemo-Sero-Therap. Res. Ctr.
    Inventors: Toshinobu Nouchi, Hiroshi Mizokami, Yoshitaka Tajima, Hiroyuki Yokote, Satoshi Adachi, Yoshinobu Miyatsu, Tetsuro Tanabe, Yoshitaka Ushio, Shinichi Shibata
  • Publication number: 20020183492
    Abstract: Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.
    Type: Application
    Filed: June 20, 2002
    Publication date: December 5, 2002
    Applicant: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
    Inventors: Satoshi Adachi, Hiroshi Mizokami, Yoshitaka Tajima, Yoshinobu Miyatsu, Toshinobu Nouchi, Yoshitaka Ushio
  • Publication number: 20020182680
    Abstract: A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.
    Type: Application
    Filed: June 20, 2002
    Publication date: December 5, 2002
    Applicant: Juridical Fdn. The Chemo-Sero-Therap. Res. Ctr.
    Inventors: Toshinobu Nouchi, Hiroshi Mizokami, Yoshitaka Tajima, Yoshinobu Miyatsu, Masahiro Sakaguchi, Kazunari Yagi
  • Publication number: 20020169294
    Abstract: The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
    Type: Application
    Filed: March 21, 2002
    Publication date: November 14, 2002
    Inventors: Hiroshi Mizokami, Shinichi Furukawa, Keishin Sugawara, Tatsufumi Onchi, Kazuhiro Komatsu, Satoshi Koyanagi, Hideo Yoshizaki
  • Patent number: 6139838
    Abstract: The present invention provides a t-PA medicinal preparation obtained by remarkably improving the solubility and stability of t-PA or modified t-PA. Nicotinamide or a derivative thereof is incorporated into t-PA or modified t-PA-containing solvent system. If necessary, a citrate buffer solution is also used. The t-PA-containing medicinal composition is in the form of, for example, a freeze-dried preparation.
    Type: Grant
    Filed: September 5, 1997
    Date of Patent: October 31, 2000
    Assignee: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
    Inventors: Kazuyuki Nakashima, Yoshitaka Tajima, Shinichi Furukawa, Hiroshi Mizokami
  • Patent number: D503381
    Type: Grant
    Filed: July 21, 2003
    Date of Patent: March 29, 2005
    Assignee: Denyo Co., Ltd.
    Inventors: Hiroshi Mizokami, Koji Suga