Abstract: It is intended to provide a novel unialgal culture strain showing a high culture efficiency of a large-sized red alga which is immature, can be stored and cultured over a long period of time and has at least one of the following properties, i.e., producing a physiologically active substance at a high yield, showing a high growth speed of the alga body, and being highly capable of absorbing nutritional salts. Namely, an immature unialgal culture strain originating in a large-sized red marine alga which is characterized by showing no matured female gametophyte in nature but showing matured tetrasporophyte alone and grows in a natural marine water area containing fresh water. This unialgal culture strain is constructed by collecting the matured sporophyte, cutting the sporophyte and allowing to stand to thereby release spores, culturing the released spores and continuously proliferating and culturing even after the growth of an upright body from the germinated spore.

Abstract: A method in which an aqueous water-soluble compound solution containing at least one impurity in a slight amount is passed through a column packed with an adsorbent onto which the impurity is selectively adsorbed to thereby remove the impurity from the aqueous solution, wherein an abnormal phenomenon in adsorption chromatography is caused to obtain an elute fraction having a higher concentration of the trace impurity than the raw solution and this fraction is removed. Thus, the trace impurity can be efficiently removed and the water-soluble compound can be obtained in a high-purity state. Applying this method to aqueous sodium chloride solutions gives sodium chloride crystals reduced in impurity concentration. Dissolving such sodium chloride crystals, which have a low potassium ion concentration, in water provides an aqueous sodium chloride solution for medical use.

Abstract: The present invention provides a novel method for recovery of photo-inhibited immunocompetence, which comprises administration of a specific active ingredient exhibiting physiological activity such as activity of activating cellular immunocompetence, which is obtained from a raw material that exists in a large amount in the nature and can easily be acquired. The above active ingredient is obtained in the form of a liquid crude active fraction by: adding ammonium sulfate to an extract obtained from Gracilaria sp., using an aqueous salt solution, to a final concentration of 20% to 40% saturated solution to conduct a first stage of salting-out, so as to eliminate the precipitated contaminants; further adding ammonium sulfate to the extract to a final concentration of 60% to 80% saturated solution to conduct a second stage of salting-out, so as to recover a crude active fraction as a precipitate; and dissolving the precipitate in a suitable solvent.

Abstract: In conducting liquid chromatographic analysis of a saccharide mixture containing monosaccharides and oligosaccharides, elution is conducted by using two kinds or more of mobile phases to separate the saccharide mixture into individual constituent saccharides followed by conversion thereof into corresponding derivatives by reaction with reagents and detection of the derivatives as contained in a detector cell. The method of the invention is characterized in that the detector cell is cleaned by washing with a cleaning solvent after completion of the detection of each of the saccharide derivatives.

Abstract: In conducting liquid chromatographic analysis of a saccharide mixture containing monosaccharides and oligosaccharides, elution is conducted by using two kinds or more of mobile phases to separate the saccharide mixture into individual constituent saccharides followed by conversion thereof into corresponding derivatives by reaction with reagents and detection of the derivatives as contained in a detector cell. The method of the invention is characterized in that the detector cell is cleaned by washing with a cleaning solvent after completion of the detection of each of the saccharide derivatives.