Abstract: The present invention relates to a method of estimating the number of urinary podocytes, including detecting podocalyxin in a urinary sediment sample liquid, and more specifically, to a method of estimating the number of urinary podocytes, including the following steps (1) to (3): (1) a step of preparing the urinary sediment sample liquid by separating urinary sediment from urine collected from a test subject and solubilizing podocalyxin in the urinary sediment; (2) a step of calculating a podocalyxin excretion amount in the urinary sediment sample liquid through detection of podocalyxin in the urinary sediment sample liquid; and (3) a step of calculating the number of urinary podocytes by dividing the podocalyxin excretion amount in the urinary sediment sample liquid by a podocalyxin amount per podocyte.

Abstract: A pneumatic tire includes a tread portion with a designated rotational direction. The tread portion is provided with a shoulder main groove arranged nearby a tread edge, a plurality of shoulder lateral grooves each extending from the shoulder main groove to the tread edge to form a plurality of shoulder blocks. Each of the shoulder blocks includes a zigzag leading edge including an external corner with a first vertex formed between a long inclined side and a short inclined side. One of the shoulder blocks is provided with a plurality of shoulder sipes extending at an angle of 0 to 20 degrees with respect to an axial direction of the tire. One of the shoulder sipes includes a deep portion and a shallow portion that is arranged so as to include a position in the axial direction of the first vertex.

Abstract: The present invention relates to a method of estimating the number of urinary podocytes, including detecting podocalyxin in a urinary sediment sample liquid, and more specifically, to a method of estimating the number of urinary podocytes, including the following steps (1) to (3): (1) a step of preparing the urinary sediment sample liquid by separating urinary sediment from urine collected from a test subject and solubilizing podocalyxin in the urinary sediment; (2) a step of calculating a podocalyxin excretion amount in the urinary sediment sample liquid through detection of podocalyxin in the urinary sediment sample liquid; and (3) a step of calculating the number of urinary podocytes by dividing the podocalyxin excretion amount in the urinary sediment sample liquid by a podocalyxin amount per podocyte.

Abstract: A server device is provided that includes a registration unit configured to accept information identifying a target application for pre-registration and information identifying a user from a terminal device and register the accepted information, and a providing unit configured to provide registration information to a management service for performing a verification with respect to the information identifying the target application and the information identifying the user accepted from the terminal device after the target application is released. The registration information may be provided in a batch or each time the verification is performed.

Abstract: Provided is a test method for the detection of diabetic nephropathy at an early stage as compared to a conventional method. Specifically provided are: a test method for diabetic nephropathy, including detecting urinary podocalyxin; the test method, further including assessing diabetic nephropathy at at least Stage I; a test reagent for use in the test method; and a test reagent kit for use in the test method. The present invention is based on a finding that urinary podocalyxin reflects the development and condition of diabetic nephropathy with high sensitivity at an early stage as compared to urinary albumin.

Abstract: Provided is a test method for the assessment of the necessity of renal biopsy in a subject to be tested, who is suspected of having a renal disease. Specifically provided are a test method for a renal disease, including using urinary podocalyxin and one or more additional markers in combination, and a test reagent for use in the test method and a test reagent kit for use in the test method. The present invention allows the discrimination of a poor prognosis group even for poor prognosis cases with no overt findings in a conventional test method, and thus allows the assessment of a renal disease, the assessment of the necessity of renal biopsy, prognostic prediction, and the like to be performed exactly.

Abstract: An object of the present invention is to provide a method for increasing the sensitivity of an immunoassay system. In order to achieve the object, the present inventors have discovered that the sensitivity of an immunoassay system can be increased by pretreating urine and thus have completed the present invention.

Abstract: The object of the present invention is to provide a method for estimating the glomerular renal function in a convenient and non-invasive manner. As a result of intensive studies to achieve the above object, the present inventors found that there is a high correlation between the urinary megalin excretion rate and the estimated glomerular filtration rate (eGFR) in renal disease patients, and the glomerular filtration rate (GFR) can be estimated with high probability in a non-invasive manner by measuring the megalin level in the urine. This has led to the completion of the present invention.

Abstract: An object of the present invention is to provide a method for increasing the sensitivity of an immunoassay system. In order to achieve the object, the present inventors have discovered that the sensitivity of an immunoassay system can be increased by pretreating urine and thus have completed the present invention.

Abstract: To improve a continuing rate of usage of an app that is executed on a terminal device. A server apparatus includes a banner request accepting unit that accepts, from a first application program executed by a terminal device, a banner request with a user ID that specifies a user of the terminal device; an application program selecting unit that selects, by referring to a usage history of the user specified by the user ID based on the user ID regarding an arbitrary application program, a second application program for which a predetermined period has passed after final activation thereof; and an advertising information sending unit that sends advertising information to encourage use of the second application program, corresponding to the selected second application program, to the terminal device, and has the advertising information displayed on a screen of the first application program.

Abstract: The present invention relates to a method of detecting urinary podocytes with a light microscope through use of an insoluble carrier-bound antibody that recognizes a protein specifically expressed on a podocyte surface, and more specifically, to a method of detecting urinary podocytes, including the following steps of (1) to (3) : (1) preparing an insoluble carrier-bound antibody by binding an insoluble carrier to an antibody that recognizes a protein specifically expressed on a podocyte surface; (2) bringing a urine sample of a test subject into contact with the insoluble carrier-bound antibody; and (3) observing the urine sample brought into contact with the insoluble carrier-bound antibody with a light microscope to count podocytes.

Abstract: The object of the present invention is to provide a method for estimating the glomerular renal function in a convenient and non-invasive manner. As a result of intensive studies to achieve the above object, the present inventors found that there is a high correlation between the urinary megalin excretion rate and the estimated glomerular filtration rate (eGFR) in renal disease patients, and the glomerular filtration rate (GFR) can be estimated with high probability in a non-invasive manner by measuring the megalin level in the urine. This has led to the completion of the present invention.

Abstract: An object of the present invention is to provide a method for increasing the sensitivity of an immunoassay system. In order to achieve the object, the present inventors have discovered that the sensitivity of an immunoassay system can be increased by pretreating urine and thus have completed the present invention.

Abstract: A pneumatic tire includes a tread portion with a designated rotational direction. The tread portion is provided with a shoulder main groove arranged nearby a tread edge, a plurality of shoulder lateral grooves each extending from the shoulder main groove to the tread edge to form a plurality of shoulder blocks. Each of the shoulder blocks includes a zigzag leading edge including an external corner with a first vertex formed between a long inclined side and a short inclined side. One of the shoulder blocks is provided with a plurality of shoulder sipes extending at an angle of 0 to 20 degrees with respect to an axial direction of the tire. One of the shoulder sipes includes a deep portion and a shallow portion that is arranged so as to include a position in the axial direction of the first vertex.

Abstract: A server device is provided that includes a registration unit configured to accept information identifying a target application for pre-registration and information identifying a user from a terminal device and register the accepted information, and a providing unit configured to provide registration information to a management service for performing a verification with respect to the information identifying the target application and the information identifying the user accepted from the terminal device after the target application is released. The registration information may be provided in a batch or each time the verification is performed.

Abstract: A technique is provided that involves issuing an advertisement code to be posted on an advertisement medium or an invite code issued in response to a request from a user, generating a parameter for associating the advertisement code or the invite code with a predetermined reward, and generating page data and returning the page data upon being accessed by a browser application of a terminal device based on an address specified by the advertisement code or the invite code. The page data is associated with a description of operations for passing the parameter corresponding to the advertisement code or the invite code and starting a predetermined game application.

Abstract: A pneumatic tire comprising a plurality of protector ribs 9 disposed on outer surface 3a of at least one sidewall portion 3 at intervals in the circumferential direction, each protector rib 9 projecting axially outwardly, extending at an angle ? of 5 to 25° with respect to the radial direction, and including outer wall 10 extending from a radially outer edge 13 having a circumferential length L1 of 10 to 25 mm, to maximum projecting portion 14 at which thickness t1 of the rib 9 reaches a maximum, and in a meridian cross section of the tire in a normal loaded state, the outer wall 10 being parallel to flat surface M or extending from the outer edge 13 toward the maximum projecting portion 14 with inclination in a direction away from the flat surface M at an angle of at most 15° with respect to the flat surface M.

Abstract: Provided is a test method for acute kidney injury, including detecting urinary podocalyxin. According to the test method, a subject to be tested who has a higher value for the urinary podocalyxin than a reference value can be assessed to have acute kidney injury. Further, as compared to a conventional method, the test method allows acute kidney injury to be assessed accurately and non-invasively, which allows a physical burden on a patient to be reduced. Thus, the test method is useful.

Abstract: This invention provides a diagnostic kit and a diagnostic marker used for diagnosing a renal disease. This invention also provides a method for detecting a renal disease comprising measuring at least one type of human megalin existing in urine selected from among full-length human megalin and human megalin fragments of (i) to (iii): (i) full-length human megalin; (ii) a human megalin endodomain fragment lacking a human megalin ectodomain; and (iii) a human megalin ectodomain fragment lacking a human megalin endodomain.

Abstract: This invention provides a simple means for detecting a renal disorder, a diagnostic marker for a renal disorder that enables prognostic prediction of a renal disorder (e.g., diabetic nephropathy and IgA nephropathy) and evaluation of the degree of nephropathy at the phase of stage-II diabetic nephropathy by measuring the megalin level in urine associated with a renal disorder used for the detection means, and use of such marker. The invention also provides the use of human megalin obtained from the urine sample of a subject as a marker for detecting a renal disorder.