Abstract: A process for producing polymer particles having an antigen or an antibody introduced into the surface thereof, by carrying out miniemulsion polymerization using a monomer, a radical polymerization initiator, an emulsifier, and a hydrophobe in the presence of the antigen or antibody to thereby produce the polymer particles, is disclosed. According to the process, it is possible to provide a reagent for immunological analysis having an excellent detection sensitivity and capable of avoiding a nonspecific reaction which occurs in conventional methods.

Abstract: A process for producing polymer particles having an antigen or an antibody introduced into the surface thereof, by carrying out miniemulsion polymerization using a monomer, a radical polymerization initiator, an emulsifier, and a hydrophobe in the presence of the antigen or antibody to thereby produce the polymer particles, is disclosed. According to the process, it is possible to provide a reagent for immunological analysis having an excellent detection sensitivity and capable of avoiding a nonspecific reaction which occurs in conventional methods.

Abstract: A reagent for immunological analysis, comprising a suspension of latex particles having an antigen or an antibody specific for a substance to be analyzed introduced into the surface of the latex particles, produced by polymerizing a monomer in the presence of the antigen or antibody to thereby synthesize the latex particles; and a method for immunological analysis, comprising the step of bringing, in a liquid, (1) a sample suspected of containing a substance to be analyzed, into contact with (2) latex particles having an antigen or an antibody specific for the substance introduced into the surface of the latex particles, produced by polymerizing a monomer in the presence of the antigen or antibody to thereby synthesize the latex particles; are disclosed. The reagent and the method have an excellent detection sensitivity, and can avoid a nonspecific reaction which occurs in conventional methods.

Abstract: The present invention provides such a measuring method of the CK isoform and a reagent therefor. Accordingly, the present invention provides a reagent for measuring creatine kinase (CK) activity comprising an antibody, wherein said antibody inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.The present invention also provides a method for measuring CK activity comprising determining an inhibition of CK of body fluids using a reagent for measuring CK activity, wherein said reagent comprises an antibody which inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.

Abstract: A reagent for assaying creatine kinase, which contains glucokinase or hexokinase, glucose-6-phosphate dehydrogenase, adenosine 5'-diphosphate, creatine phosphate, oxidized nicotinamide adenine dinucleotide phosphate, magnesium salt, and glucose; and which comprises a first liquid reagent having a pH value of 7.5 to 10 and containing at least glucokinase and/or hexokinase, glucose-6-phosphate dehydrogenase, adenosine 5'-diphosphate and creatine phosphate, and a second liquid reagent having a pH value of 2 to 5 and containing at least oxidized nicotinamide adenine dinucleotide phosphate. The present reagent can be stably stored in the form of liquid for a long period of time at normal or low temperature in dark or light place. Therefore, it can be used without dissolution procedure after being preserved for a long term at the site of clinical examination and is also applicable to automatic analyzers.

Abstract: The present invention provides such a measuring method of the CK isoform and a reagent therefor. Accordingly, the present invention provides a reagent for measuring creatine kinase (CK) activity comprising an antibody, wherein said antibody inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.The present invention also provides a method for measuring CK activity comprising determining an inhibition of CK of body fluids using a reagent for measuring CK activity, wherein said reagent comprises an antibody which inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.

Abstract: A reagent for assaying glucose, which comprises a first liquid reagent having a pH of 7.5 to 9.5 and containing at least either glucokinase or hexokinase, glucose 6-phosphate dehydrogenase, and adenosine 5'-triphosphate and a second liquid reagent having a pH of 3 to 5 and containing the oxidized form of nicotinamide adenine dinucleotide phosphate. The reagent can be stored as such stably for long in or out of contact with light at normal or low temperatures, so that it can be stored for long in the site of clinical examination and applied to an automatic analyzer without the necessity for dissolution prior to use.

Abstract: A transparent and stable aqueous solution of a substrate for a determination of lipase, comprising triglyceride uniformly solubilized therein, a method for determination of lipase activity in a sample, employing the aqueous solution, and a process for the manufacture of a lyophilized product capable of forming the aqueous solution are disclosed. A process for the manufacture of a transparent and stable aqueous solution of triglyceride is also disclosed.

Abstract: A transparent and stable aqueous solution of a substrate for a determination of lipase, comprising triglyceride uniformly solubilized therein, a method for determination of lipase activity in a sample, employing the aqueous solution, and a process for the manufacture of a lyophilized product capable of forming the aqueous solution are disclosed. A process for the manufacture of a transparent and stable aqueous solution of triglyceride is also disclosed.