Patents by Inventor Hisashi Yasueda

Hisashi Yasueda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200024629
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Application
    Filed: September 25, 2019
    Publication date: January 23, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Patent number: 9187570
    Abstract: The present invention provides means useful for making devices, materials and the like that are excellent in photocatalytic activity, electric property or the like. Specifically, the present invention provides a fusion protein comprising a polypeptide portion capable of forming a multimer having an internal cavity, and a first peptide portion capable of binding to a first target substance and a second peptide portion capable of binding to a second target substance; a multimer of the fusion protein; a complex comprising the multimer of the fusion protein; and the like.
    Type: Grant
    Filed: June 21, 2013
    Date of Patent: November 17, 2015
    Assignee: Ajinomoto Co., Ltd.
    Inventors: Ippei Inoue, Ichiro Yamashita, Bin Zheng, Hisashi Yasueda, Yukiharu Uraoka, Yasuaki Ishikawa
  • Patent number: 8993279
    Abstract: A method for producing an L-amino acid is described, which is characterized by culturing a Vibrio bacterium capable of producing the L-amino acid in a culture medium to produce and accumulate the L-amino acid in the culture medium and collecting the L-amino acid from the culture medium.
    Type: Grant
    Filed: July 31, 2009
    Date of Patent: March 31, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoko Asakura, Ippei Inoue, Hisashi Yasueda
  • Patent number: 8969048
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 3, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20140242643
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: May 2, 2014
    Publication date: August 28, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Patent number: 8753849
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: June 17, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20140150855
    Abstract: A functional material having excellent photocatalytic activity, electric characteristics and the like is provided. A porous structure body 10 comprises a first target material 20 and an aggregate body 30 formed by aggregation of the first material. The aggregate body 30 adheres to the first target material and is located so as to surround the first target material. The aggregate body has a plurality of first pores 32 unevenly distributed near the first target material in the aggregate body and a plurality of second pores 34 scattered over the aggregate body.
    Type: Application
    Filed: February 10, 2014
    Publication date: June 5, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Ippei INOUE, Ichiro Yamashita, Bin Zheng, Hisashi Yasueda, Yukiharu Uraoka, Yasuaki Ishikawa
  • Publication number: 20140045247
    Abstract: The present invention provides means useful for making devices, materials and the like that are excellent in photocatalytic activity, electric property or the like. Specifically, the present invention provides a fusion protein comprising a polypeptide portion capable of forming a multimer having an internal cavity, and a first peptide portion capable of binding to a first target substance and a second peptide portion capable of binding to a second target substance; a multimer of the fusion protein; a complex comprising the multimer of the fusion protein; and the like.
    Type: Application
    Filed: October 16, 2013
    Publication date: February 13, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: IPPEI INOUE, ICHIRO YAMASHITA, BIN ZHENG, HISASHI YASUEDA, YUKIHARU URAOKA, YASUAKI ISHIKAWA
  • Patent number: 8404474
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: June 28, 2010
    Date of Patent: March 26, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20120276598
    Abstract: A method for efficiently producing an L-amino acid, especially L-lysine, by using a ?-proteobacterium is provided. In a method for producing an L-amino acid comprising culturing a bacterium belonging to ?-Proteobacteria and having an ability to produce an L-amino acid, for example, an Enterobacteriaceae bacterium such as Escherichia coli, in a medium containing glycerol as a carbon source to produce and accumulate the L-amino acid in the medium, and collecting the L-amino acid from the medium, a bacterium modified so that the activity of the Cnu protein is reduced is used as the bacterium.
    Type: Application
    Filed: May 4, 2012
    Publication date: November 1, 2012
    Inventors: Ippei Inoue, Hisashi Yasueda
  • Publication number: 20120156736
    Abstract: L-Lysine is produced by culturing in a medium a Vibrio bacterium which has an ability to produce L-lysine, and has been modified so that an activity of a protein encoded by the fucO gene is reduced to produce and accumulate L-lysine in the medium or cells of the bacterium, and collecting L-lysine from the medium or cells.
    Type: Application
    Filed: February 2, 2012
    Publication date: June 21, 2012
    Inventors: Ippei Inoue, Hisashi Yasueda
  • Patent number: 8080396
    Abstract: A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase ?-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.
    Type: Grant
    Filed: September 8, 2009
    Date of Patent: December 20, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seizaburo Shiraga, Noriko Murayama, Hiroshi Izui, Hisao Ito, Hisashi Yasueda, Yoshihiro Usuda, Kazuhiko Matsui
  • Patent number: 8017363
    Abstract: A method for production of L-lysine is provided which includes the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium contains DNA encoding dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a LysE protein that can enhance the excretion of L-lysine out of the methanol-utilizing bacterium, and the bacterium is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: September 13, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshiya Gunji, Hisashi Yasueda, Reiko Hirai, Seiko Hirano
  • Patent number: 8012722
    Abstract: L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.
    Type: Grant
    Filed: July 25, 2008
    Date of Patent: September 6, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Akito Chinen, Hisashi Yasueda, Jun Nakamura
  • Patent number: 7993885
    Abstract: A DNA encoding a variant of a protein having a loop region and six hydrophobic helixes which is involved in excretion of L-lysine to outside of a cell is described, wherein the DNA encodes a mutant protein which does not contain the loop region that is present in the wild-type protein. The mutant protein facilitates excretion of L-lysine, L-arginine, or both to the outside of the cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium. Specifically, lysE24 is introduced into a methanol assimilating bacterium such as Methylophilus bacteria which results in improved L-amino acid productivity, especially production of L-lysine and L-arginine.
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: August 9, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshiya Gunji, Hisashi Yasueda
  • Publication number: 20100267094
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: June 28, 2010
    Publication date: October 21, 2010
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Patent number: 7785858
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: August 10, 2005
    Date of Patent: August 31, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20100190216
    Abstract: A method for production of L-lysine is provided which includes the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium contains DNA encoding dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a LysE protein that can enhance the excretion of L-lysine out of the methanol-utilizing bacterium, and the bacterium is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.
    Type: Application
    Filed: August 1, 2008
    Publication date: July 29, 2010
    Inventors: Yoshiya Gunji, Hisashi Yasueda, Reiko Hirai, Seiko Hirano
  • Publication number: 20100099152
    Abstract: L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.
    Type: Application
    Filed: July 25, 2008
    Publication date: April 22, 2010
    Inventors: Akito Chinen, Hisashi Yasueda, Jun Nakamura
  • Publication number: 20100062497
    Abstract: A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase ?-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.
    Type: Application
    Filed: September 8, 2009
    Publication date: March 11, 2010
    Inventors: Seizaburo Shiraga, Noriko Murayama, Hiroshi Izui, Hisao Ito, Hisashi Yasueda, Yoshihiro Usuda, Kazuhiko Matsui