Patents by Inventor Hisatake Okada
Hisatake Okada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11435348Abstract: It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.Type: GrantFiled: December 16, 2019Date of Patent: September 6, 2022Assignee: KONICA MINOLTA, INC.Inventors: Hideki Gouda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
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Patent number: 11378517Abstract: A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.Type: GrantFiled: April 11, 2018Date of Patent: July 5, 2022Assignee: KONICA MINOLTA, INC.Inventors: Kensaku Takanashi, Hideki Goda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi
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Patent number: 11275087Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.Type: GrantFiled: November 8, 2017Date of Patent: March 15, 2022Assignee: KONICA MINOLTA, INC.Inventors: Hideki Gouda, Masaru Takahashi, Kensaku Takanashi, Hisatake Okada, Yuichi Ozaki, Yuka Yoshihara, Yasushi Nakano, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe, Norikazu Masuda, Masakazu Toi, Hiroshi Tada, Minoru Miyashita
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Publication number: 20210404919Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.Type: ApplicationFiled: September 8, 2021Publication date: December 30, 2021Inventors: Takuji AIMIYA, Hideki GOUDA, Hisatake OKADA, Yasushi NAKANO, Kohsuke GONDA, Motohiro TAKEDA, Noriaki OHUCHI
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Publication number: 20210311024Abstract: An object of the present invention is to provide a non-clinical test method which allows for the quality control of experimental animals having a transplanted tumor site, such as tumor-bearing mice, or experimental animals having a lesion site other than a transplanted a tumor site, and which is characterized by including the step of identifying, using a specimen collected from such an experimental animal, the profile of a lesion site, for example, a transplanted tumor site, of the experimental animal, by a quantitative technique.Type: ApplicationFiled: February 15, 2017Publication date: October 7, 2021Applicant: Konica Minolta, Inc.Inventors: Hideki GOUDA, Takeshi SHIRAISHI, Takeshi ISODA, Noboru KOYAMA, Hisatake OKADA
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Publication number: 20210041446Abstract: The present invention provides a method of obtaining information for the diagnosis or treatment, mainly of cancer or an immune system-related disease, the method including the step of quantifying the expression level of a cancer-associated protein or a nucleic acid in a tumor cell; and/or the step of quantifying the expression level of a protein or a nucleic acid in an immune cell, using a specimen derived from tumor tissue of a human or a non-human.Type: ApplicationFiled: February 15, 2017Publication date: February 11, 2021Inventors: Masaru TAKAHASHI, Etsuko FUTAYA, Hisatake OKADA, Noboru KOYAMA
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Publication number: 20210003486Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.Type: ApplicationFiled: September 24, 2020Publication date: January 7, 2021Inventors: Takuji AIMIYA, Hideki GOUDA, Hisatake OKADA, Yasushi NAKANO, Kohsuke GONDA, Motohiro TAKEDA, Noriaki OHUCHI
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Patent number: 10809167Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.Type: GrantFiled: March 15, 2011Date of Patent: October 20, 2020Assignee: KONICA MINOLTA, INC.Inventors: Takuji Aimiya, Hideki Gouda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi
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Patent number: 10627325Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.Type: GrantFiled: February 19, 2015Date of Patent: April 21, 2020Assignee: KONICA MINOLTA, INC.Inventors: Takuji Aimiya, Hideki Gouda, Hisatake Okada, Yasushi Nakano
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Publication number: 20200116718Abstract: It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.Type: ApplicationFiled: December 16, 2019Publication date: April 16, 2020Inventors: Hideki GOUDA, Hisatake OKADA, Yasushi NAKANO, Kohsuke GONDA, Noriaki OHUCHI, Mika WATANABE
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Patent number: 10551386Abstract: The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.Type: GrantFiled: January 5, 2017Date of Patent: February 4, 2020Assignee: KONICA MINOLTA, INC.Inventors: Kensaku Takanashi, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
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Patent number: 10551378Abstract: It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.Type: GrantFiled: September 4, 2012Date of Patent: February 4, 2020Assignee: KONICA MINOLTA, INC.Inventors: Hideki Gouda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
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Publication number: 20180231466Abstract: A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.Type: ApplicationFiled: April 11, 2018Publication date: August 16, 2018Inventors: Kensaku TAKANASHI, Hideki GODA, Hisatake OKADA, Yasushi NAKANO, Kohsuke GONDA, Motohiro TAKEDA, Noriaki OHUCHI
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Patent number: 9976959Abstract: A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.Type: GrantFiled: August 30, 2011Date of Patent: May 22, 2018Assignees: KONICA MINOLTA MEDICAL & GRAPHIC, INC., KONICA MINOLTA, INC.Inventors: Kensaku Takanashi, Hideki Goda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi
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Publication number: 20180136212Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.Type: ApplicationFiled: November 8, 2017Publication date: May 17, 2018Inventors: Hideki GOUDA, Masaru TAKAHASHI, Kensaku TAKANASHI, Hisatake OKADA, Yuichi OZAKI, Yuka YOSHIHARA, Yasushi NAKANO, Kohsuke GONDA, Noriaki OHUCHI, Mika WATANABE, Norikazu MASUDA, Masakazu TOI, Hiroshi TADA, Minoru MIYASHITA
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Publication number: 20170115300Abstract: The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.Type: ApplicationFiled: January 5, 2017Publication date: April 27, 2017Inventors: Kensaku TAKANASHI, Hisatake OKADA, Yasushi NAKANO, Kohsuke GONDA, Noriaki OHUCHI, Mika WATANABE
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Patent number: 9483684Abstract: A medical image processor and storage medium are shown. According to one implementation, a medical image processor includes an input unit, an operation unit, a cell nucleus extracting unit, a fluorescent bright point extracting unit, a feature amount calculating unit, and an output unit. The input unit is used to input a cell shape image showing a shape of a cell and a fluorescent image showing expression of a specific protein as a fluorescent bright point. The operation unit is used to specify an analysis target region. The cell nucleus extracting unit extracts a region of a cell nucleus. The fluorescent bright point extracting unit extracts a fluorescent bright point. The feature amount calculating unit calculates a feature amount showing an expression amount of the specific protein. The output unit outputs the calculated feature amount.Type: GrantFiled: March 27, 2013Date of Patent: November 1, 2016Assignee: KONICA MINOLTA, INC.Inventors: Akinori Tsunomori, Hisatake Okada, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
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Patent number: 9244076Abstract: The present invention provides a fluorescent label that can be used for carrying out a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The fluorescent label is a fluorescent dye-containing nanoparticle in which the parent material is a cross-linked polymer and the fluorescent dye is an aromatic ring-based dye molecule. The cross-linked polymer is suitably a melamine resin or a styrene resin. The aromatic ring-based dye molecule is suitably a perylene and more suitably a perylene diimide.Type: GrantFiled: March 7, 2014Date of Patent: January 26, 2016Assignee: KONICA MINOLTA, INC.Inventors: Kensaku Takanashi, Hisatake Okada, Yasushi Nakano
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Patent number: 9189678Abstract: A medical image processor and a storage medium are shown. According to one implementation, the medical image processor includes the following. An input unit is used to input a cell shape image and a fluorescent image showing expression of a specific protein. A cell nucleus extracting unit extracts a cell nucleus. A fluorescent bright point extracting unit extracts a fluorescent bright point. A region estimating unit sets a predetermined region. When the set region does not overlap with another, it is estimated to include one cell. When a plurality of the set regions overlap, it is estimated to include a plurality of cells. A feature amount calculating unit calculates a feature amount. A determining unit determines whether each estimated cell region is cancer and determines an expression status in the region based on the calculated feature amount. An output unit outputs a determination result.Type: GrantFiled: March 27, 2013Date of Patent: November 17, 2015Assignee: KONICA MINOLTA, INC.Inventors: Akinori Tsunomori, Hisatake Okada, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
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Publication number: 20150160103Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.Type: ApplicationFiled: February 19, 2015Publication date: June 11, 2015Inventors: Takuji AIMIYA, Hideki Gouda, Hisatake Okada, Yasushi Nakano