Abstract: A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues, without substantially increasing the viscosity of the solution, by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: A method for producing a medium composition for suspension culture of an adherent cell, including the following steps: (i) a step of making an extracellular matrix carried on a nanofiber composed of water-insoluble polysaccharides, (ii) a step of adding the extracellular matrix-carrying nanofiber obtained in step (i) to a medium is provided by the present invention.

Abstract: The present invention provides a method capable of easily mixing any liquid containing a linking substance such as a divalent metal cation and the like with a liquid containing a particular compound at a high concentration, and capable of producing a liquid medium composition comprising fine structures dispersed therein, and a production device therefor and a kit therefor. The first liquid containing a particular compound is passed through a through-hole having a given cross-sectional area formed in a nozzle part at a given flow rate and injected into the second liquid at a given flow rate. By this simple operation, a structure in which the particular compound is bonded via the linking substance is formed, and the structure is preferably dispersed in a mixture of the both liquids.

Abstract: The present invention provides a method capable of easily mixing any liquid containing a linking substance such as a divalent metal cation and the like with a liquid containing a particular compound at a high concentration, and capable of producing a liquid medium composition comprising fine structures dispersed therein, and a production device therefor and a kit therefor. The first liquid containing a particular compound is passed through a through-hole having a given cross-sectional area formed in a nozzle part at a given flow rate and injected into the second liquid at a given flow rate. By this simple operation, a structure in which the particular compound is bonded via the linking substance is formed, and the structure is preferably dispersed in a mixture of the both liquids.

Abstract: The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Abstract: Cells or tissues are preserved in an unfrozen state in the liquid composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion and the like.

Abstract: A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes.

Abstract: A cell culture system for dividing and subculturing cell aggregates is constituted by connecting a first container 10 and a divider 20. An outlet of a divider is connected to the first container. A mesh structure 22 for dividing a cell aggregate is provided in a conduit for dividing in the divider 20, and a cell aggregate is divided while passing through the mesh structure. The divided cell aggregate is sent to the first container 10 without contacting the outside air in a preferred embodiment, where further culture is performed. This makes it possible to divide the cell aggregate and perform suspension culture while maintaining the closed system. The division and suspension culture of cell aggregates can be repeated by sending the cultured cell aggregates to a divider of its own cell culture system or a divider of a cell culture system prepared separately.

Abstract: The device has a film-shaped main body part 1, and predetermined region in the film surface of the main body part has a mesh structure in which a large number of through-holes 20 are arranged. The through-hole has an opening shape having a size allowing smaller cell aggregates to pass through, and the rest of the through-hole is the beam part 30. The beam part is a part that cuts a cell aggregate to be divided, and is integrally connected to form a network. The cell aggregate can be divided by passing the cell aggregate to be divided through the mesh structure of the device together with the liquid.

Abstract: Cells or tissues are preserved in an unfrozen state in the liquid composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion and the like.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: The present invention provides a cell carrier containing a nanofiber composed of water-insoluble polysaccharides, preferably chitin or chitosan nanofiber; a carrier capable of being a common carrier in various operations such as i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, v) recovery of bioactive substance from culture supernatant and the like of adherent cells; and continuous performance of plural operations selected from i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, and v) recovery of bioactive substance from culture supernatant of adherent cells by using the carrier.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: Provided is a method for producing a liquid medium composition, including using a junction conduit structure 10 having a structure in which a first inlet conduit 11 and a second inlet conduit 12 are joined together to form an outlet conduit 13, and flowing a first liquid 1 containing a particular compound into the first inflow conduit 11 and flowing a second liquid 2 containing a linking substance into the second inlet conduit 12, and allowing the flows of the both liquids to join together to mix the both liquids, thus forming a liquid medium composition 3 containing structures formed by the particular compound linked via the linking substance dispersed therein while being flown through the outlet conduit 13.

Abstract: A culture method (a preservation method) of somatic stem cells does not use a chemical such as DMSO that affects a differentiation function of somatic stem cells. A material for an undifferentiated state-maintaining culture for a somatic stem cell, having a naturally occurring polysaccharide; a culture liquid in which the material for the undifferentiated state-maintaining culture is dispersed; a somatic stem cell-containing culture liquid in which the somatic stem cell is suspended in the culture liquid; and a culture method for mesenchymal stem cells in which a naturally occurring polysaccharide is used.

Abstract: The present invention provides a production method of a protein including suspension culture of a cell having protein production ability in a medium composition containing nanofiber under physical disruption conditions and in a state of being attached to the nanofiber.

Abstract: The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Abstract: Described are a method for producing cellulose fibers suitable for use in a thickening composition, a method for producing a thickening composition that contains the cellulose fibers, and a method for thickening a composition. The method for producing the cellulose fibers includes grinding a composition containing cellulose fibers and an aqueous solution to produce a dispersion containing cellulose fibers having an average fiber diameter (D) in a range of from 0.001 to 100 ?m in the aqueous solution.