Patents by Inventor Hisato HIRANO
Hisato HIRANO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230279374Abstract: A mutant SaCas9 protein such as a protein having an amino acid sequence resulting from mutations of glutamic acid at the 782-position to lysine (E782K), leucine at the 800-position to arginine (L800R), asparagine at the 968-position to arginine (N968R), asparagine at the 985-position to alanine (N985A), arginine at the 991-position to alanine (R991A), alanine at the 1021-position to serine (A1021S), threonine at the 927-position to lysine (T927K), lysine at the 929-position to asparagine (K929N), and isoleucine at the 1017-position to phenylalanine (I1017F) in SEQ ID NO: 2 has relaxed restriction on target sequence while maintaining binding ability to guide RNA, and is useful as a tool for gene editing.Type: ApplicationFiled: November 25, 2022Publication date: September 7, 2023Applicants: THE UNIVERSITY OF TOKYO, MODALIS THERAPEUTICS CORPORATIONInventors: Osamu NUREKI, Hiroshi NISHIMASU, Hisato HIRANO, Shohei KAJIMOTO, Tetsuya YAMAGATA, Yuanbo QIN, Keith M. CONNOLLY, Iain THOMPSON
-
Patent number: 11702645Abstract: The present invention aims to provide a modified Cas9 protein with relaxed restriction on target sequence while maintaining binding ability to guide RNA, and use thereof. A protein containing the amino acid sequence of SEQ ID NO: 1 in which the 1335-position arginine is mutated into alanine (R1335A), isoleucine (R1335I), methionine (R1335M), threonine (R1335T) or valine (R1335V), the 1111-position leucine is mutated into arginine (L1111R), the 1135-position aspartic acid is mutated into valine (D1135V), the 1218-position glycine is mutated into arginine (G1218R), the 1219-position glutamic acid is mutated into phenylalanine (E1219F), the 1322-position alanine is mutated into arginine (A1322R), and the 1337-position threonine is mutated into arginine (T1337R), and the like.Type: GrantFiled: June 24, 2022Date of Patent: July 18, 2023Assignee: The University of TokyoInventors: Osamu Nureki, Hiroshi Nishimasu, Hisato Hirano
-
Patent number: 11530396Abstract: A mutant SaCas9 protein such as a protein having an amino acid sequence resulting from mutations of glutamic acid at the 782-position to lysine (E782K), leucine at the 800-position to arginine (L800R), asparagine at the 968-position to arginine (N968R), asparagine at the 985-position to alanine (N985A), arginine at the 991-position to alanine (R991A), alanine at the 1021-position to serine (A1021S), threonine at the 927-position to lysine (T927K), lysine at the 929-position to asparagine (K929N), and isoleucine at the 1017-position to phenylalanine (I1017F) in SEQ ID NO: 2 has relaxed restriction on target sequence while maintaining binding ability to guide RNA, and is useful as a tool for gene editing.Type: GrantFiled: September 5, 2018Date of Patent: December 20, 2022Assignees: THE UNIVERSITY OF TOKYO, MODALIS THERAPEUTICS CORPORATIONInventors: Osamu Nureki, Hiroshi Nishimasu, Hisato Hirano, Shohei Kajimoto, Tetsuya Yamagata, Yuanbo Qin, Keith M. Connolly, Iain Thompson
-
Publication number: 20220333090Abstract: The present invention aims to provide a modified Cas9 protein with relaxed restriction on target sequence while maintaining binding ability to guide RNA, and use thereof. A protein containing the amino acid sequence of SEQ ID NO: 1 in which the 1335-position arginine is mutated into alanine (R1335A), isoleucine (R1335I), methionine (R1335M), threonine (R1335T) or valine (R1335V), the 1111-position leucine is mutated into arginine (L1111R), the 1135-position aspartic acid is mutated into valine (D1135V), the 1218-position glycine is mutated into arginine (G1218R), the 1219-position glutamic acid is mutated into phenylalanine (E1219F), the 1322-position alanine is mutated into arginine (A1322R), and the 1337-position threonine is mutated into arginine (T1337R), and the like.Type: ApplicationFiled: June 24, 2022Publication date: October 20, 2022Applicant: The University of TokyoInventors: Osamu NUREKI, Hiroshi NISHIMASU, Hisato HIRANO
-
Patent number: 11371030Abstract: The present invention aims to provide a modified Cas9 protein with relaxed restriction on target sequence while maintaining binding ability to guide RNA, and use thereof. A protein containing the amino acid sequence of SEQ ID NO: 1 in which the 1335-position arginine is mutated into alanine (R1335A), isoleucine (R1335I), methionine (R1335M), threonine (R1335T) or valine (R1335V), the 1111-position leucine is mutated into arginine (L1111R), the 1135-position aspartic acid is mutated into valine (D1135V), the 1218-position glycine is mutated into arginine (G1218R), the 1219-position glutamic acid is mutated into phenylalanine (E1219F), the 1322-position alanine is mutated into arginine (A1322R), and the 1337-position threonine is mutated into arginine (T1337R), and the like.Type: GrantFiled: May 31, 2018Date of Patent: June 28, 2022Assignee: The University of TokyoInventors: Osamu Nureki, Hiroshi Nishimasu, Hisato Hirano
-
Publication number: 20210163907Abstract: A mutant SaCas9 protein such as a protein having an amino acid sequence resulting from mutations of glutamic acid at the 782-position to lysine (E782K), leucine at the 800-position to arginine (L800R), asparagine at the 968-position to arginine (N968R), asparagine at the 985-position to alanine (N985A), arginine at the 991-position to alanine (R991A), alanine at the 1021-position to serine (A1021S), threonine at the 927-position to lysine (T927K), lysine at the 929-position to asparagine (K929N), and isoleucine at the 1017-position to phenylalanine (I1017F) in SEQ ID NO: 2 has relaxed restriction on target sequence while maintaining binding ability to guide RNA, and is useful as a tool for gene editing.Type: ApplicationFiled: September 5, 2018Publication date: June 3, 2021Applicants: THE UNIVERSITY OF TOKYO, MODALIS THERAPEUTICS CORPORATIONInventors: Osamu NUREKI, Hiroshi NISHIMASU, Hisato HIRANO, Shohei KAJIMOTO, Tetsuya YAMAGATA, Yuanbo QIN, Keith M. CONNOLLY, Iain THOMPSON
-
Publication number: 20200277586Abstract: The present invention aims to provide a modified Cas9 protein with relaxed restriction on target sequence while maintaining binding ability to guide RNA, and use thereof. A protein containing the amino acid sequence of SEQ ID NO: 1 in which the 1335-position arginine is mutated into alanine (R1335A), isoleucine (R1335I), methionine (R1335M), threonine (R1335T) or valine (R1335V), the 1111-position leucine is mutated into arginine (L1111R), the 1135-position aspartic acid is mutated into valine (D1135V), the 1218-position glycine is mutated into arginine (G1218R), the 1219-position glutamic acid is mutated into phenylalanine (E1219F), the 1322-position alanine is mutated into arginine (A1322R), and the 1337-position threonine is mutated into arginine (T1337R), and the like.Type: ApplicationFiled: May 31, 2018Publication date: September 3, 2020Applicant: The University of TokyoInventors: Osamu NUREKI, Hiroshi NISHIMASU, Hisato HIRANO
-
Publication number: 20180201912Abstract: The present invention provides a protein comprising a sequence containing any one of the amino acid sequences of the following (a) to (f) and having RNA-guided DNA endonuclease activity.Type: ApplicationFiled: July 14, 2016Publication date: July 19, 2018Applicant: The University of TokyoInventors: Osamu NUREKI, Hiroshi NISHIMASU, Hisato HIRANO, Ryuichiro ISHITANI