Abstract: A process enables efficient preparation of liposome-encapsulated peptide P33. The process comprising preparing a peptide P33; preparing a lipid mixture by dissolving 70-90 mg of phospholipid including granulated soy lecithin and 20 mg of cholesterol in 15-25 mg of a chloroform/methanol solvent system; evaporating organic solvent using a magnetic stirrer coupled with a heating system until a lipid film is fully formed and hydrated; removing solvent traces by drying the lipid film under vacuum conditions overnight; rehydrating the lipid film with 8-12 mL of a buffer containing 190-210 ?g/mL of peptide P33; mixing the rehydrated lipid film thoroughly using a magnetic stirrer at ambient temperature to ensure uniform dispersion; sonicating the lipid suspension until a clear, translucent solution is obtained; and extruding the resulting suspension using a vortex to form large unilamellar vesicles (LUVs) with a uniform size of approximately 200 nm.

Abstract: The system comprises a collection unit for procuring doxorubicin, diethyl ether, alkaline water and a plurality of subject animals; a pre-processor unit for acclimatizing collected plurality of subject animals for 7 days standard laboratory condition; a classification processor unit for dividing subject animals into four groups; a treating unit for feeding subject animals with standard semi purified diet along with normal drinking water and standard semi purified diet along with Zam water p.o at libitum, or injecting with Dox i.p. on first day of protocol fed with standard semi purified diet along with normal drinking water or Dox i.p. on first day of protocol thereby treating with Zam water p.o at libitum for 28 days; a monitoring processor unit for monitoring physiological and behavioral changes of plurality of subject animals; and an analysis processor unit for estimating a set of biochemical parameters and stool sample of the plurality of subject animals.