Abstract: In a sound processing method of operating audio equipment to perform sound processing relating to audio content in accordance with one embodiment, a distributor terminal communicates with the audio equipment and at least one listener terminal, the distributor terminal distributes the audio content from the distributor terminal to the at least one listener terminal, the distributor terminal specifies an operation listener terminal, from among the at least one listener terminal, that is permitted to change a sound processing parameter of the audio equipment, the operation listener terminal accepts the sound processing parameter changed by the operation listener terminal, the operation listener terminal transmits the sound processing parameter changed by the operation listener terminal to the distributor terminal, and the audio equipment performs the sound processing based on the changed sound processing parameter changed by the operation listener terminal.

Abstract: The object of the invention is to provide an anaerobic enterobacterium having a higher therapeutic effect on an anaerobic site such as a solid tumor tissue and an ischemic disease site. A bacterium of the genus Bifidobacterium, which is transformed with a plasmid co-expressing two types of heterologous polypeptides and comprising two types of secretory expression cassettes each sequentially comprising a promoter DNA functioning in the bacterium of the genus Bifidobacterium; a DNA encoding a secretory signal peptide; a DNA encoding a heterologous polypeptide; and a terminator DNA functioning in the bacterium of the genus Bifidobacterium, efficiently secretes the two types of heterologous peptides, i.e., two types of antibodies having anticancer effects, outside the bacterial cell.

Abstract: An object is to provide a signal peptide that can secrete a heterologous polypeptide with high efficiency outside the bacterial cell in a bacterium of the genus Bifidobacterium, an expression cassette that can secrete a heterologous polypeptide with high efficiency outside the bacterial cell, a heterologous polypeptide expression vector, a bacterium of the genus Bifidobacterium capable of secreting a heterologous polypeptide. Means for attaining the object is a bacterium of the genus Bifidobacterium transformed by a vector having an expression cassette sequentially comprising a promoter DNA functioning in a bacterium of the genus Bifidobacterium, a DNA encoding the secretory signal peptide, a DNA encoding a scFv antibody having an antitumor activity, and a terminator DNA functioning in the bacterium of the genus Bifidobacterium; and capable of secreting the scFv antibody with high efficiency outside the bacterial cell.

Abstract: It is an object of the present invention to provide signal sequence information capable of secreting an antibody to the outside of cells in generation of the antibody by microorganisms of genus Bifidobacterium, and an antibody expression vector capable of secreting an antibody to the outside of cells by utilizing the signal sequence information. As a means for achieving the aforementioned object, there is prepared Bifidobacterium longum, which is transformed with a vector having inserted thereinto a DNA insert comprising the 5?-terminus of an antibody gene linked to the 3?-terminus of a DNA encoding a signal peptide-linker conjugate having a linker linked to the C-terminus of a signal peptide consisting of an amino acid sequence shown in SEQ ID NO: 1.

Abstract: The object of the invention is to provide an anaerobic enterobacterium having a higher therapeutic effect on an anaerobic site such as a solid tumor tissue and an ischemic disease site. A bacterium of the genus Bifidobacterium, which is transformed with a plasmid co-expressing two types of heterologous polypeptides and comprising two types of secretory expression cassettes each sequentially comprising a promoter DNA functioning in the bacterium of the genus Bifidobacterium; a DNA encoding a secretory signal peptide; a DNA encoding a heterologous polypeptide; and a terminator DNA functioning in the bacterium of the genus Bifidobacterium, efficiently secretes the two types of heterologous peptides, i.e., two types of antibodies having anticancer effects, outside the bacterial cell.

Abstract: [Object] To provide a gene transfer carrier that specifically accumulates only at a disease site even with systemic administration, has a high protein expression rate, and disappears from the disease site accompanying a cure, and a method for the treatment of an ischemic disease using same. [Solution means] The above-mentioned object has been accomplished by providing a gene transfer carrier formed from an anaerobic bacterium that can grow specifically at the site of an ischemic disease and can express at least one type of protein that is useful for the diagnosis or treatment of an ischemic disease, a pharmaceutical composition containing the gene transfer carrier, and a treatment method for an ischemic disease utilizing same.

Abstract: It is an object of the present invention to provide signal sequence information capable of secreting an antibody to the outside of cells in generation of the antibody by microorganisms of genus Bifidobacterium, and an antibody expression vector capable of secreting an antibody to the outside of cells by utilizing the signal sequence information. As a means for achieving the aforementioned object, there is prepared Bifidobacterium longum, which is transformed with a vector having inserted thereinto a DNA insert comprising the 5?-terminus of an antibody gene linked to the 3?-terminus of a DNA encoding a signal peptide-linker conjugate having a linker linked to the C-terminus of a signal peptide consisting of an amino acid sequence shown in SEQ ID NO: 1.

Abstract: An object is to provide a signal peptide that can secrete a heterogeneous polypeptide with high efficiency outside the bacterial cell in a bacterium of the genus Bifidobacterium, an expression cassette that can secrete a heterogeneous polypeptide with high efficiency outside the bacterial cell, a heterogeneous polypeptide expression vector, a bacterium of the genus Bifidobacterium capable of secreting a heterogeneous polypeptide. Means for attaining the object is a bacterium of the genus Bifidobacterium transformed by a vector having an expression cassette sequentially comprising a promoter DNA functioning in a bacterium of the genus Bifidobacterium, a DNA encoding the secretory signal peptide, a DNA encoding a scFv antibody having an antitumor activity, and a terminator DNA functioning in the bacterium of the genus Bifidobacterium; and capable of secreting the scFv antibody with high efficiency outside the bacterial cell.

Abstract: The present invention relates to a novel secretory signal, a novel plasmid containing the secretory signal, a transformed anaerobic bacterium transformed with said plasmid, a gene transfer carrier consisting of said anaerobic bacterium, and a pharmaceutical composition containing said carrier.

Abstract: To provide a gene transfer carrier that specifically accumulates only at a disease site even with systemic administration, has a high protein expression rate, and disappears from the disease site accompanying a cure, and a method for the treatment of an ischemic disease using same. The above-mentioned object has been accomplished by providing a gene transfer carrier formed from an anaerobic bacterium that can grow specifically at the site of an ischemic disease and can express at least one type of protein that is useful for the diagnosis or treatment of an ischemic disease, a pharmaceutical composition containing the gene transfer carrier, and a treatment method for an ischemic disease utilizing same.

Abstract: Provided is a method of producing an ?-olefin polymer including a step of polymerizing one or more kinds of ?-olefins each having 6 to 20 carbon atoms with a catalyst obtained by using a specific transition metal compound. By the method, an ?-olefin polymer having a viscosity suitable for use in a lubricating oil can be produced on an industrial scale with ease, and further, the characteristics of the product can be widely changed through the control of reaction conditions.

Abstract: Provided are an ?-olefin copolymer useful as a high-viscosity lubricant oil excellent in viscosity characteristics, low-temperature characteristics and oxidation stability, and a lubricant oil containing the copolymer. The ?-olefin copolymer is a 1-octene/1-decene copolymer produced by the use of a doubly bridged metallocene catalyst.

Abstract: Provided are an ?-olefin copolymer useful as a high-viscosity lubricant oil excellent in viscosity characteristics, low-temperature characteristics and oxidation stability, and a lubricant oil containing the copolymer. The ?-olefin copolymer is a 1-octene/1-decene/1-dodecene ternary copolymer produced by the use of a doubly crosslinked metallocene catalyst.

Abstract: Provided are an ?-olefin copolymer useful as a high-viscosity lubricant oil excellent in viscosity characteristics, low-temperature characteristics and oxidation stability, and a lubricant oil containing the copolymer. The ?-olefin copolymer is a 1-decene/1-dodecene copolymer produced by the use of a doubly bridged metallocene catalyst.

Abstract: The present invention relates to a secretory signal, a plasmid containing the secretory signal, a transformed anaerobic bacterium transformed with said plasmid, a gene transfer carrier consisting of said anaerobic bacterium, and a pharmaceutical composition containing said carrier.

Abstract: The present invention provides a therapeutic agent for anaerobic diseases such as solid tumor, comprising in combination a pharmaceutical composition for the treatment of an anaerobic disease containing the transformed anaerobic microorganism as an active component and a pharmaceutical composition containing as an active component an anaerobic microorganism colonization and growth enhancer for enhancing the specific colonization and proliferation of the anaerobic microorganism at an anaerobic disease site. Furthermore, the present invention provides to an anaerobic microorganism colonization and growth enhancer for enhancing colonization and growth of the transformed anaerobic microorganism at a disease site that is in an anaerobic environment.

Abstract: [Object] To provide a gene transfer carrier that specifically accumulates only at a disease site even with systemic administration, has a high protein expression rate, and disappears from the disease site accompanying a cure, and a method for the treatment of an ischemic disease using same. [Solution means] The above-mentioned object has been accomplished by providing a gene transfer carrier formed from an anaerobic bacterium that can grow specifically at the site of an ischemic disease and can express at least one type of protein that is useful for the diagnosis or treatment of an ischemic disease, a pharmaceutical composition containing the gene transfer carrier, and a treatment method for an ischemic disease utilizing same.

Abstract: A conventional shuttle vector constructed by fusing an E. coli-derived plasmid and a transformant-derived plasmid functions in both E. coli and the transformant bacterium, and there exists no expression vector that functions only in a non-E. coli transformant. The present invention provides an plasmid expression vector comprising (1) a plasmid replication unit that functions in an anaerobic microorganism other than E. coli and (2) a protein expression unit formed from DNA coding for a protein having target activity and a DNA fragment containing a promoter and a terminator that function in the anaerobic microorganism. The expression vector of the present invention is capable of being replicated only in a transformant, eliminating the risk of the replication of the transformant gene in other pathogenic or aerobic bacterium, providing an extremely safe and reliable vector and gene transporter for therapeutic application.

Abstract: Provided are an ?-olefin (co)polymer useful as high-viscosity lubricant oil excellent in viscosity characteristics and low-temperature characteristics, and a lubricant oil containing the (co)polymer. The ?-olefin (co)polymer is produced by the use of a metallocene catalyst and satisfies the following (a?) and (b?). (a?) The 2,1-insertion ratio at the polymer terminal, as measured through 1H-NMR, is at least 30 mol % of the entire molecule, and (b?) the mesotriad fraction (mm), as measured through 13C-NMR, is at most 50 mol %. Preferably, a 1-octene/1-dodecene copolymer is provided, as produced by the use of a metallocene catalyst and satisfying the following (a) to (c). (a) The molar ratio of the 1-octene unit to the 1-dodecene unit is from 20/80 to 80/20, (b) the mesotriad fraction (mm), as measured through 13C-NMR, is at most 50 mol %, and (c) the kinematic viscosity at 100° C. is from 30 to 1000 mm2/sec.

Abstract: Provided is a method of producing an ?-olefin polymer including a step of polymerizing one or more kinds of ?-olefins each having 6 to 20 carbon atoms with a catalyst obtained by using a specific transition metal compound. By the method, an ?-olefin polymer having a viscosity suitable for use in a lubricating oil can be produced on an industrial scale with ease, and further, the characteristics of the product can be widely changed through the control of reaction conditions.