Patents by Inventor Hitoshi Chiba
Hitoshi Chiba has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230333125Abstract: A problem of the present invention is to provide a diagnostic biomarker of nonalcoholic steatohepatitis (NASH) useful for differentiation of simple steatosis (SS) from NASH and use thereof. Specifically, the present invention relates to a diagnostic biomarker of nonalcoholic steatohepatitis used to differentiate nonalcoholic steatohepatitis from simple steatosis comprising the oxidized apolipoprotein A-I (ApoA-I) modified by dioxidation of the 72nd tryptophan in the ApoA-I consisting of the amino acid sequence as shown in SEQ ID NO: 1.Type: ApplicationFiled: June 15, 2021Publication date: October 19, 2023Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, EIKEN KAGAKU KABUSHIKI KAISHAInventors: Toshihiro SAKURAI, Shu-Ping HUI, Hitoshi CHIBA
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Publication number: 20210199676Abstract: Provided are a method of assisting the detection of nonalcoholic steatohepatitis (NASH) and a method that is able to assist in determining the degree of progression of any condition associated with NASH, which are far less invasive than liver biopsy and include simple operations and are independent of the skills of technicians. The present invention provides a method of assisting the detection of nonalcoholic steatohepatitis, including measuring the abundance of LDL-TG and/or ApoE-rich HDL-C contained in a test blood sample isolated from a living body.Type: ApplicationFiled: August 22, 2019Publication date: July 1, 2021Applicants: Denka Company Limited, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Kazuhiro NOUSO, Yasuki ITO, Motoko OHTA, Hitoshi CHIBA, Toshihiro SAKURAI, Hiroyuki OKADA
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Patent number: 9890412Abstract: Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.Type: GrantFiled: April 10, 2013Date of Patent: February 13, 2018Assignees: Denka Seiken Co., Ltd., National University Corporation Hokkaido UniversityInventors: Yasuki Itoh, Hitoshi Chiba
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Patent number: 9145578Abstract: This invention provides a method for separately or simultaneously quantifying cholesterol in a total amount of HDL-C, cholesterol in an HDL subfraction of apoE-containing HDL-C, and cholesterol in an HDL subfraction of apoE-deficient HDL-C. The method comprises enzymatically and separately quantifying cholesterol in apoE-containing HDL and cholesterol in apoE-deficient HDL by adding a surfactant selected from the group consisting of a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 0.7 to less than 1.3, a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of less than 0.7, and a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 1.3 or more to a test sample, allowing cholesterol esterase and cholesterol oxidase to react therewith, and quantifying the hydrogen peroxide generated.Type: GrantFiled: October 26, 2010Date of Patent: September 29, 2015Assignees: Denka Seiken Co., Ltd., National University Corporation Hokkaido UniversityInventors: Yasuki Itoh, Hitoshi Chiba
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Patent number: 9012513Abstract: Problem To provide an antioxidant, an antioxidant composition, and a method for producing the antioxidant and the antioxidant composition, which feature a high content rate and degree of extraction of substances. These substances are taurine, glycogen, protein, so-called blood platelet anticoagulant with zinc, fat-soluble vitamin with a high activation such as vitamin D, and other useful substances. The antioxidant and the antioxidant composition also feature a so-called antioxidative property, which has recently attracted attention. Solution The present invention, for example, includes 3,5-dihydroxy-4-methoxybenzyl alcohol.Type: GrantFiled: January 26, 2012Date of Patent: April 21, 2015Assignees: Watanabe Oyster Laboratory Co., Ltd., National University Corporation Hokkaido UniversityInventors: Mitsugu Watanabe, Hitoshi Chiba, Hirotoshi Fuda, Shigeki Jin
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Publication number: 20150079616Abstract: Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.Type: ApplicationFiled: April 10, 2013Publication date: March 19, 2015Applicants: Denka Seiken Co., Ltd., National University Corporation Hokkaido UniversityInventors: Yasuki Itoh, Hitoshi Chiba
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Patent number: 8729240Abstract: Provided is a monoclonal antibody against slightly oxidized LDL, which can play a role as an important tool in the research and development of oxidized LDL. Also provided are a kit for the simple detection of slightly oxidized LDL and a method for the simple detection of slightly oxidized LDL from the biological sample of a subject to be tested which use the monoclonal antibody. By means of ELISA (Enzyme-Linked Immunosorbent Assay) using the monoclonal antibody as the solid phase antibody and an anti-apolipoprotein B antibody as the detection antibody, the degree of reaction between a severely oxidized low-density lipoprotein and the monoclonal antibody is low in comparison to the degree of reaction between a slightly oxidized low-density lipoprotein and the monoclonal antibody, and the monoclonal antibody specifically reacts with an oxidized low-density lipoprotein.Type: GrantFiled: April 28, 2011Date of Patent: May 20, 2014Assignee: National University Corporation Hokkaido UniversityInventors: Hitoshi Chiba, Seiichi Kobayashi, Hiroyuki Furukawa
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Publication number: 20130303629Abstract: Problem To provide an antioxidant, an antioxidant composition, and a method for producing the antioxidant and the antioxidant composition, which feature a high content rate and degree of extraction of substances. These substances are taurine, glycogen, protein, so-called blood platelet anticoagulant with zinc, fat-soluble vitamin with a high activation such as vitamin D, and other useful substances. The antioxidant and the antioxidant composition also feature a so-called antioxidative property, which has recently attracted attention. Solution The present invention, for example, includes 3,5-dihydroxy-4-methoxybenzyl alcohol.Type: ApplicationFiled: January 26, 2012Publication date: November 14, 2013Inventors: Mitsugu Watanabe, Hitoshi Chiba, Hirotoshi Fuda, Shigeki Jin
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Publication number: 20130253174Abstract: Provided is a monoclonal antibody against slightly oxidized LDL, which can play a role as an important tool in the research and development of oxidized LDL. Also provided are a kit for the simple detection of slightly oxidized LDL and a method for the simple detection of slightly oxidized LDL from the biological sample of a subject to be tested which use the monoclonal antibody. By means of ELISA (Enzyme-Linked Immunosorbent Assay) using the monoclonal antibody as the solid phase antibody and an anti-apolipoprotein B antibody as the detection antibody, the degree of reaction between a severely oxidized low-density lipoprotein and the monoclonal antibody is low in comparison to the degree of reaction between a slightly oxidized low-density lipoprotein and the monoclonal antibody, and the monoclonal antibody specifically reacts with an oxidized low-density lipoprotein.Type: ApplicationFiled: April 28, 2011Publication date: September 26, 2013Applicant: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hitoshi Chiba, Seiichi Kobayashi, Hiroyuki Furukawa
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Publication number: 20120208219Abstract: This invention provides a method for separately or simultaneously quantifying cholesterol in a total amount of HDL-C, cholesterol in an HDL subfraction of apoE-containing HDL-C, and cholesterol in an HDL subfraction of apoE-deficient HDL-C. The method comprises enzymatically and separately quantifying cholesterol in apoE-containing HDL and cholesterol in apoE-deficient HDL by adding a surfactant selected from the group consisting of a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 0.7 to less than 1.3, a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of less than 0.7, and a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 1.3 or more to a test sample, allowing cholesterol esterase and cholesterol oxidase to react therewith, and quantifying the hydrogen peroxide generated.Type: ApplicationFiled: October 26, 2010Publication date: August 16, 2012Inventors: Yasuki Itoh, Hitoshi Chiba
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Publication number: 20120118761Abstract: Disclosed is a method for measuring the concentration of a peroxide using a CNT sensor. The CNT sensor comprises a working electrode that is arranged on an insulating substrate, a monolayered carbon nano-tube that is contacted with the working electrode, a counter electrode, and a reference electrode. A sample is provided on the monolayered carbon nano-tube, and a potential difference is made between the working electrode and the counter electrode. In this manner, the concentration of the peroxide in the sample can be measured. The measurement method can be applied to clinical tests or the like.Type: ApplicationFiled: July 16, 2010Publication date: May 17, 2012Applicants: Health Sciences University of Hokkaido, National University Corporation Hokkaido UniversityInventors: Hitoshi Chiba, Sheiji Takeda, Atsushi Ishii, Motonori Nakamura
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Patent number: 5435435Abstract: An improved adjustable conveyor belt has a plurality of modules having intermeshing link ends extending lengthwise. The modules are connected together by connecting pins extending widthwise through holes formed in the link ends. Each of the modules also has side edges and a slot adjacent to each of its side edges. Thus, slots are provided along the side edges of the belt regardless of the arrangement of the modules. A stop, removably fitted into slots located along the side edges of the belt, prevents widthwise movement of the connecting pins. The stop has a guide mechanism for guiding the stop into the slot, an end portion which extends into the pin insertion hole, an engaging portion which engages a tongue formed in the slot, and a protrusion which snaps into the pin insertion hole. This configuration makes it easy to assemble, add to, and dismantle the conveyor belt, without substantially increasing the size or weight of the assembly.Type: GrantFiled: July 7, 1994Date of Patent: July 25, 1995Assignee: Tsubakimoto Chain Co.Inventors: Hitoshi Chiba, Tomofumi Ohtani, Toshio Takahashi, Kenji Kose, Katsutoshi Shibayama, Masaaki Ikeda
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Patent number: 4475842Abstract: A corrosion resistant shaft and boss clamping device comprises an inner race to be fit over the shaft, an outer race to be fit inside the boss enclosing the shaft and the inner race, the races having wedge-shaped surfaces facing one another, a pair of taper rings having a complementary wedge shape disposable between races, and, bolts adapted to urge the taper rings toward one another, thereby clamping the shaft to the boss. The inner and outer races are expansible and contractable, being made of split rings of austenitic stainless steel. The clamping bolts are made of precipitation hardened stainless steel, and one of the taper rings is provided with a hardened nitrided layer on the surface thereof to be contacted by the clamping surfaces of the clamping bolts.Type: GrantFiled: March 18, 1982Date of Patent: October 9, 1984Assignee: Tsubakimoto Chain CompanyInventors: Mamoru Onaya, Hitoshi Chiba, Toshihiro Hosokawa