Abstract: The present invention relates to a method for assessing respiratory viral infections, the method includes preparing one or more precision-cut human bronchial tissues; exposing a cilia-rich epithelium within the precision-cut human bronchial tissues to establish an ex vivo model of human bronchus for viral infections; assessing infectivity, tropism, and pathogenesis of one or more respiratory viruses in the ex vivo model; and providing a semi-quantitative and normalized approach to supplement data from the ex vivo model of human bronchus for pandemic risk assessment of the one or more respiratory viruses. The present invention involves micro-dissection of bronchial tissues to expose the cilia-rich epithelium for ex vivo virus infection, along with a semi-quantitative approach for analyzing virus tropism and replication competence.

Abstract: The present invention relates to a non-invasive method for generating human three-dimensional nasopharyngeal organoids, including non-invasively collecting nasopharyngeal swab samples; culturing the samples in a matrix with niche factors; and obtaining the three-dimensional human nasopharyngeal organoids after culturing the samples in the matrix for 14-21 days. The three-dimensional human nasopharyngeal organoids are then dissociated into a single cell suspension for the creation of human two-dimensional nasopharyngeal organoids. The present nasopharyngeal organoids and their preparation method enable the rapid expansion of stem cells to form organoids within a short period of time. By combining organoid passaging techniques, a sufficient number of organoids can be obtained within a limited time for research and experimental operations.