Abstract: The invention provides methods, kits, and related compositions for rapid, low-cost, point of care detection of an organism, such as a virus or bacteria, in a biological or environmental sample using isothermal nucleic acid amplification.

Abstract: Devices, systems, and methods for a treating dementia or Alzheimer's disease in a subject in need thereof. In one example, combined auditory and visual stimuli having a frequency of about 20 Hz to about 60 Hz, and more specifically about 40 Hz, are non-invasively delivered to the subject to induce synchronized gamma oscillations in at least one brain region of the subject. In particular, pursuant to various treatment and exposure protocols, combined auditory and visual stimulation (as opposed to auditory or visual stimulation alone) promotes a microglia response in the medial prefrontal cortex (mPFC). More generally, combined auditory and visual stimulation induces an extended microglia clustering response in the auditory cortex, the visual cortex, and the mPFC.

Abstract: A method of treating neurodegeneration in a subject that includes administering a non-invasive tactile stimulus having a stimulus frequency of about 30 Hz to about 50 Hz to a subject to induce synchronized gamma oscillations in at least one portion of the peripheral nervous system of the subject, of the spinal cord of the subject, or both.

Abstract: Devices, systems, and methods for a treating dementia or Alzheimer's disease in a subject in need thereof. In one example, combined auditory and visual stimuli having a frequency of about 20 Hz to about 60 Hz, and more specifically about 40 Hz, are non-invasively delivered to the subject to induce synchronized gamma oscillations in at least one brain region of the subject. In particular, pursuant to various treatment and exposure protocols, combined auditory and visual stimulation (as opposed to auditory or visual stimulation alone) promotes a microglia response in the medial prefrontal cortex (mPFC). More generally, combined auditory and visual stimulation induces an extended microglia clustering response in the auditory cortex, the visual cortex, and the mPFC.

Abstract: In an automated methodology for in vivo image-guided cell patch clamping, a cell patch clamping device is moved into position and targeted to a specific cell using automated image-guided techniques. Cell contact is determined by analyzing the temporal series of measured resistance levels at the clamping device as it is moved. The difference between successive resistance levels is compared to a threshold, which must be exceeded before cell contact is assumed. Pneumatic control methods are used to achieve gigaseal formation and cell break-in, leading to whole-cell patch clamp formation. An automated robotic system capable of performing this methodology automatically performs patch clamping in vivo, automatically locating cells through image guidance and by analyzing the temporal sequence of electrode impedance changes.

Abstract: A device is disclosed for reconstitution and delivery of an injectable pharmaceutical. A diluent is provided in a pressurized reservoir, while separately a syringe with a needle contains a pharmaceutical such as a lyophilized drug. When the pressurized reservoir is coupled to the interior of the syringe, such as by piercing the pressurized reservoir with the needle, the pressurized reservoir forces the diluent into the syringe where the diluent can mix with and reconstitute the pharmaceutical. A variety of techniques are disclosed for pressurizing the reservoir and fluidly coupling the reservoir with the syringe. The techniques disclosed herein may, for example, be adapted for use with a conventional syringe and needle.

Abstract: Devices, systems, and methods for a treating dementia or Alzheimer's disease in a subject in need thereof. In one example, combined auditory and visual stimuli having a frequency of about 20 Hz to about 60 Hz, and more specifically about 40 Hz, are non-invasively delivered to the subject to induce synchronized gamma oscillations in at least one brain region of the subject. In particular, pursuant to various treatment and exposure protocols, combined auditory and visual stimulation (as opposed to auditory or visual stimulation alone) promotes a microglia response in the medial prefrontal cortex (mPFC). More generally, combined auditory and visual stimulation induces an extended microglia clustering response in the auditory cortex, the visual cortex, and the mPFC.

Abstract: A device is disclosed for reconstitution and delivery of an injectable pharmaceutical. A pharmaceutical composition such as a lyophilized drug is provided in a pressurizable fluid reservoir with a pierceable seal. A diluent may be separately provided in a syringe with a needle. When the needle is driven through the seal and the plunger is actuated, fluid from the barrel of the syringe flows into the pressurizable fluid reservoir to simultaneously pressurize the reservoir and mix the diluent with the drug. Pressurized in this manner, the reservoir can then automatically and without further user intervention push the mixture back through the needle and into the barrel of the syringe, further mixing the composition and displacing the plunger to fill the barrel with the reconstituted drug. The needle and syringe can then be detached from the reservoir with the reconstituted drug contained in the barrel and ready for injection.

Abstract: Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels. The membrane sensor according to the present invention may enable mass-production of sensors and secure reliability of detection by forming the plurality of channels on the membrane by a simple method.

Abstract: In an automated methodology for in vivo image-guided cell patch clamping, a cell patch clamping device is moved into position and targeted to a specific cell using automated image-guided techniques. Cell contact is determined by analyzing the temporal series of measured resistance levels at the clamping device as it is moved. The difference between successive resistance levels is compared to a threshold, which must be exceeded before cell contact is assumed. Pneumatic control methods are used to achieve gigaseal formation and cell break-in, leading to whole-cell patch clamp formation. An automated robotic system capable of performing this methodology automatically performs patch clamping in vivo, automatically locating cells through image guidance and by analyzing the temporal sequence of electrode impedance changes.

Abstract: A device is disclosed for reconstitution and delivery of an injectable pharmaceutical. A pharmaceutical composition such as a lyophilized drug is provided in a pressurizable fluid reservoir with a pierceable seal. A diluent may be separately provided in a syringe with a needle. When the needle is driven through the seal and the plunger is actuated, fluid from the barrel of the syringe flows into the pressurizable fluid reservoir to simultaneously pressurize the reservoir and mix the diluent with the drug. Pressurized in this manner, the reservoir can then automatically and without further user intervention push the mixture back through the needle and into the barrel of the syringe, further mixing the composition and displacing the plunger to fill the barrel with the reconstituted drug. The needle and syringe can then be detached from the reservoir with the reconstituted drug contained in the barrel and ready for injection.

Abstract: Exemplary embodiments of the present invention relate to light emitting diodes including a plurality of light emitting cells on a substrate to be suitable for AC driving. The light emitting diode includes a substrate and a plurality of light emitting cell formed on the substrate. Each light emitting cell includes a first region at a boundary of the light emitting cell and a second region opposite to the first region. A first electrode pad is formed in the first region of the light emitting cell. A second electrode pad having a linear shape is disposed to face the first electrode pad while regionally defining a peripheral region together with the boundary of the second region. A wire connects the first electrode pad to the second electrode pad between two adjacent light emitting cells.

Abstract: A device is disclosed for reconstitution and delivery of an injectable pharmaceutical. A diluent is provided in a pressurized reservoir, while separately a syringe with a needle contains a pharmaceutical such as a lyophilized drug. When the pressurized reservoir is coupled to the interior of the syringe, such as by piercing the pressurized reservoir with the needle, the pressurized reservoir forces the diluent into the syringe where the diluent can mix with and reconstitute the pharmaceutical. A variety of techniques are disclosed for pressurizing the reservoir and fluidly coupling the reservoir with the syringe. The techniques disclosed herein may, for example, be adapted for use with a conventional syringe and needle.

Abstract: Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels. The membrane sensor according to the present invention may enable mass-production of sensors and secure reliability of detection by forming the plurality of channels on the membrane by a simple method.

Abstract: Exemplary embodiments of the present invention relate to light emitting diodes including a plurality of light emitting cells on a substrate to be suitable for AC driving. The light emitting diode includes a substrate and a plurality of light emitting cell formed on the substrate. Each light emitting cell includes a first region at a boundary of the light emitting cell and a second region opposite to the first region. A first electrode pad is formed in the first region of the light emitting cell. A second electrode pad having a linear shape is disposed to face the first electrode pad while regionally defining a peripheral region together with the boundary of the second region. A wire connects the first electrode pad to the second electrode pad between two adjacent light emitting cells.

Abstract: Exemplary embodiments of the present invention relate to light emitting diodes including a plurality of light emitting cells on a substrate to be suitable for AC driving. The light emitting diode includes a substrate and a plurality of light emitting cell formed on the substrate. Each light emitting cell includes a first region at a boundary of the light emitting cell and a second region opposite to the first region. A first electrode pad is formed in the first region of the light emitting cell. A second electrode pad having a linear shape is disposed to face the first electrode pad while regionally defining a peripheral region together with the boundary of the second region. A wire connects the first electrode pad to the second electrode pad between two adjacent light emitting cells.

Abstract: Exemplary embodiments of the present invention relate to light emitting diodes including a plurality of light emitting cells on a substrate to be suitable for AC driving. The light emitting diode includes a substrate and a plurality of light emitting cell formed on the substrate. Each light emitting cell includes a first region at a boundary of the light emitting cell and a second region opposite to the first region. A first electrode pad is formed in the first region of the light emitting cell. A second electrode pad having a linear shape is disposed to face the first electrode pad while regionally defining a peripheral region together with the boundary of the second region. A wire connects the first electrode pad to the second electrode pad between two adjacent light emitting cells.