Abstract: The disclosure provides for methods of synthesizing a heterofunctional biolinker comprising converting a cyclohexane carboxylic acid into an carboxylic acid alkyl halide; reacting the carboxylic acid alkyl halide with a protected hydroxylamine to yield a protected alkoxyamine; converting the carboxylic acid into a reactive ester; and deprotecting the protected alkoxyamine to yield a heterofunctional biolinker capable of reacting exclusively with carbonyl groups in biomarkers and antibody drug conjugates. The biolinkers beneficially accommodate a wide variety of labels. Also disclosed are kits, compositions produced by the methods, and methods of using the same to detect a target.