Abstract: It is desirable to produce live vaccines, which are highly attenuated and which do only contain minimal or no animal-derived components. The production of highly attenuated live viruses can be better achieved when using specifically designed cell lines as producer substrate as opposed to using less defined primary cells. However, live viruses, thus produced comprise undesirable components from the cell lines and cell culture. The present invention relates to methods of production and purification of live enveloped viruses, which are suitable for vaccination.

Abstract: It is desirable to produce live vaccines, which are highly attenuated and which do only contain minimal or no animal-derived components. The production of highly attenuated live viruses can be better achieved when using specifically designed cell lines as producer substrate as opposed to using less defined primary cells. However, live viruses, thus produced comprise undesirable components from the cell lines and cell culture. The present invention relates to methods of production and purification of live enveloped viruses, which are suitable for vaccination.

Abstract: A device for invalidating planar security documents is provided. The device has a horizontal base plate (9) onto which objects (14) to be invalidated are placed, and die plates (13, 17, 18) which are vertically movable and parallel to the base plate. The device has a perforating apparatus supported on the base plate and the die plates, and a drive mechanism by which the die plates can be displaced up and down. The perforating apparatus has at least one cutting bushing (23) with a hollow paper punch (19) interacting with each cutting bushing in order to displace the paper scrap in the paper punch. The upper end of each paper punch (19) is open and provides an exit for the paper scrap into an upper storage cavity for containing the scrap. The cavity is in a support (03), having a vacuum connection, and is connectable to a vacuum apparatus for removal of the paper scrap.