Patents by Inventor Holger Grallert

Holger Grallert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11860158
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: January 2, 2024
    Assignee: BIOMÉRIEUX DEUTSCHLAND GMBH
    Inventors: Johannes Reich, Holger Grallert
  • Patent number: 11116824
    Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: September 14, 2021
    Assignee: HYpharm GmbH
    Inventors: Holger Grallert, Sonja Molinaro
  • Patent number: 11098298
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Grant
    Filed: May 1, 2019
    Date of Patent: August 24, 2021
    Assignee: BIOMÉRIEUX DEUTSCHLAND GMBH
    Inventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
  • Publication number: 20210018495
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Application
    Filed: September 29, 2020
    Publication date: January 21, 2021
    Applicant: HYGLOS INVEST GMBH
    Inventors: Johannes REICH, Holger GRALLERT
  • Patent number: 10697958
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: June 30, 2020
    Assignee: HYGLOS INVEST GMBH
    Inventors: Johannes Reich, Holger Grallert
  • Publication number: 20190381151
    Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.
    Type: Application
    Filed: June 27, 2019
    Publication date: December 19, 2019
    Inventors: Holger Grallert, Sonja Molinaro
  • Publication number: 20190353648
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Application
    Filed: July 31, 2019
    Publication date: November 21, 2019
    Applicant: HYGLOS INVEST GMBH
    Inventors: Johannes REICH, Holger GRALLERT
  • Publication number: 20190256836
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Application
    Filed: May 1, 2019
    Publication date: August 22, 2019
    Applicant: HYGLOS INVEST GMBH
    Inventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
  • Patent number: 10342857
    Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.
    Type: Grant
    Filed: June 15, 2018
    Date of Patent: July 9, 2019
    Assignee: HYPHARM GMBH
    Inventors: Holger Grallert, Sonja Molinaro
  • Publication number: 20190187131
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Application
    Filed: February 22, 2019
    Publication date: June 20, 2019
    Applicant: HYGLOS INVEST GMBH
    Inventors: Johannes REICH, Holger GRALLERT
  • Patent number: 10323238
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Grant
    Filed: June 28, 2017
    Date of Patent: June 18, 2019
    Assignee: HYGLOS INVEST GMBH
    Inventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
  • Publication number: 20180333471
    Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.
    Type: Application
    Filed: June 15, 2018
    Publication date: November 22, 2018
    Inventors: Holger Grallert, Sonja Molinaro
  • Patent number: 10022430
    Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: July 17, 2018
    Assignee: HYpharm GmbH
    Inventors: Holger Grallert, Sonja Molinaro
  • Publication number: 20170306315
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Application
    Filed: June 28, 2017
    Publication date: October 26, 2017
    Applicant: HYGLOS INVEST GMBH
    Inventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
  • Patent number: 9725706
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harboring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Grant
    Filed: December 4, 2013
    Date of Patent: August 8, 2017
    Assignee: HYGLOS INVEST GMBH
    Inventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
  • Publication number: 20170131265
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Application
    Filed: June 12, 2015
    Publication date: May 11, 2017
    Applicant: HYGLOS INVEST GMBH
    Inventors: Johannes REICH, Holger GRALLERT
  • Patent number: 9518252
    Abstract: The present invention relates to a novel Listeria bacteriophage designated ProCC P825. In particular, the present invention relates to the endolysin PlyP825 encoded by the novel phage ProCC P825 and uses of the novel endolysin PlyP825 for controlling Listeria contamination and infection.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: December 13, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Holger Grallert, Julia Lorenz, Anna Scherzinger
  • Patent number: 9229002
    Abstract: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.
    Type: Grant
    Filed: July 29, 2014
    Date of Patent: January 5, 2016
    Assignee: Hyglos Invest GmbH
    Inventors: Stefan Miller, Roman Meyer, Renate Grassl, Manfred Biebl, Holger Grallert
  • Publication number: 20150299684
    Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.
    Type: Application
    Filed: December 4, 2013
    Publication date: October 22, 2015
    Applicant: MICROCOAT BIOTECHNOLOGIE GMBH
    Inventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
  • Publication number: 20150238577
    Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.
    Type: Application
    Filed: May 11, 2015
    Publication date: August 27, 2015
    Inventors: Holger Grallert, Sonja Molinaro