Patents by Inventor Holger Grallert
Holger Grallert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11860158Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: February 22, 2019Date of Patent: January 2, 2024Assignee: BIOMÉRIEUX DEUTSCHLAND GMBHInventors: Johannes Reich, Holger Grallert
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Patent number: 11116824Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.Type: GrantFiled: June 27, 2019Date of Patent: September 14, 2021Assignee: HYpharm GmbHInventors: Holger Grallert, Sonja Molinaro
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Patent number: 11098298Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: May 1, 2019Date of Patent: August 24, 2021Assignee: BIOMÉRIEUX DEUTSCHLAND GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Publication number: 20210018495Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: September 29, 2020Publication date: January 21, 2021Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Patent number: 10697958Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: June 12, 2015Date of Patent: June 30, 2020Assignee: HYGLOS INVEST GMBHInventors: Johannes Reich, Holger Grallert
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Publication number: 20190381151Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.Type: ApplicationFiled: June 27, 2019Publication date: December 19, 2019Inventors: Holger Grallert, Sonja Molinaro
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Publication number: 20190353648Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: July 31, 2019Publication date: November 21, 2019Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Publication number: 20190256836Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: May 1, 2019Publication date: August 22, 2019Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Patent number: 10342857Abstract: The present invention relates to assays, kits and oligonucleotides for the detection of Pseudomonas aeruginosa for a fast, sensitive and reliable detection of Pseudomonas aeruginosa in a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of Pseudomonas aeruginosa, a kit for the serotype-specific detection of Pseudomonas aeruginosa, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use of Pseudomonas aeruginosa serotype specific antibodies for serotype specific treatment of Pseudomonas aeruginosa infection in a patient detected for said specific Pseudomonas aeruginosa serotype with such an assay or kit.Type: GrantFiled: June 15, 2018Date of Patent: July 9, 2019Assignee: HYPHARM GMBHInventors: Holger Grallert, Sonja Molinaro
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Publication number: 20190187131Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: February 22, 2019Publication date: June 20, 2019Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Patent number: 10323238Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: June 28, 2017Date of Patent: June 18, 2019Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Publication number: 20180333471Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.Type: ApplicationFiled: June 15, 2018Publication date: November 22, 2018Inventors: Holger Grallert, Sonja Molinaro
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Patent number: 10022430Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.Type: GrantFiled: May 11, 2015Date of Patent: July 17, 2018Assignee: HYpharm GmbHInventors: Holger Grallert, Sonja Molinaro
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Publication number: 20170306315Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: June 28, 2017Publication date: October 26, 2017Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Patent number: 9725706Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harboring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: December 4, 2013Date of Patent: August 8, 2017Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Publication number: 20170131265Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: June 12, 2015Publication date: May 11, 2017Applicant: HYGLOS INVEST GMBHInventors: Johannes REICH, Holger GRALLERT
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Patent number: 9518252Abstract: The present invention relates to a novel Listeria bacteriophage designated ProCC P825. In particular, the present invention relates to the endolysin PlyP825 encoded by the novel phage ProCC P825 and uses of the novel endolysin PlyP825 for controlling Listeria contamination and infection.Type: GrantFiled: May 29, 2012Date of Patent: December 13, 2016Assignee: DSM IP ASSETS B.V.Inventors: Holger Grallert, Julia Lorenz, Anna Scherzinger
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Patent number: 9229002Abstract: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.Type: GrantFiled: July 29, 2014Date of Patent: January 5, 2016Assignee: Hyglos Invest GmbHInventors: Stefan Miller, Roman Meyer, Renate Grassl, Manfred Biebl, Holger Grallert
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Publication number: 20150299684Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: December 4, 2013Publication date: October 22, 2015Applicant: MICROCOAT BIOTECHNOLOGIE GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Publication number: 20150238577Abstract: The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens.Type: ApplicationFiled: May 11, 2015Publication date: August 27, 2015Inventors: Holger Grallert, Sonja Molinaro