Patents by Inventor Holly H. Hogrefe

Holly H. Hogrefe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood
    Patent number: 9758773
    Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.
    Type: Grant
    Filed: December 12, 2014
    Date of Patent: September 12, 2017
    Assignee: Agilent Technologies, Inc.
    Inventors: Nancy Mckinney, Holly H. Hogrefe, Jeffrey Fox, Connie J. Hansen, Bahram Arezi
  • Mutant Reverse Transcriptase and Methods of Use
    Publication number: 20160340657
    Abstract: The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.
    Type: Application
    Filed: April 4, 2016
    Publication date: November 24, 2016
    Applicant: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Bahram Arezi, Weimei Xing
  • Thermostable Type-A DNA Polymerase Mutant with Increased Resistance to Inhibitors in Blood
    Publication number: 20150232821
    Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.
    Type: Application
    Filed: December 12, 2014
    Publication date: August 20, 2015
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Nancy Mckinney, Holly H. Hogrefe, Jeffrey Fox, Connie J. Hansen, Bahram Arezi
  • Multi-site mutagenesis
    Patent number: 8685676
    Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: April 1, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • DNA polymerase compositions for quantitative PCR and methods thereof
    Patent number: 8283148
    Abstract: The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3?-5? exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3?-5? exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3?-5? exonuclease activity and reduced base analog detection activity.
    Type: Grant
    Filed: December 12, 2003
    Date of Patent: October 9, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Joseph A. Sorge, Reinhold Dietrich Mueller, Gothami Padmabandu, Nick Roelofs, Holly H. Hogrefe
  • Multi-site mutagenesis
    Patent number: 8067556
    Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
    Type: Grant
    Filed: July 18, 2002
    Date of Patent: November 29, 2011
    Assignee: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • Compositions and methods for random nucleic acid mutagenesis
    Patent number: 6803216
    Abstract: The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
    Type: Grant
    Filed: May 23, 2002
    Date of Patent: October 12, 2004
    Assignee: Stratagene California
    Inventors: Holly H. Hogrefe, Janice M. Cline
  • DNA polymerases with reduced base analog detection activity
    Publication number: 20040086890
    Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
    Type: Application
    Filed: April 7, 2003
    Publication date: May 6, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Holly H. Hogrefe, Madhushree Ghosh
  • DNA polymerases with reduced base analog detection activity
    Publication number: 20040081965
    Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal dna polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
    Type: Application
    Filed: October 25, 2002
    Publication date: April 29, 2004
    Applicant: Stratagene
    Inventors: Joseph A. Sorge, Holly H. Hogrefe, Madhushree Ghosh
  • Compositions and methods for random nucleic acid molecules
    Publication number: 20030152944
    Abstract: The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
    Type: Application
    Filed: May 23, 2002
    Publication date: August 14, 2003
    Applicant: Stratagene
    Inventors: Holly H. Hogrefe, Janice M. Cline