Patents by Inventor Holly J. Wolf

Holly J. Wolf has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • 1,2-dehydrogenation of steroidal 21-esters with Arthrobacter simplex or Bacterium cyclooxydans
    Patent number: 5225335
    Abstract: The present invention involves processes for 1,2-dehydrogenation of .DELTA..sup.4 -3-keto C.sub.21 -hemiester steroids of formula (I) ##STR1## with Arthrobacter simplex or Bacterium cyclooxydans to produce the corresponding .DELTA..sup.1,4 -3-keto C.sub.21 hemiester of formula (II) ##STR2## or free alcohol thereof with improved yields. The advantage is that the processes utilize higher substrate concentrations and give shorter reaction times than previously were known.
    Type: Grant
    Filed: September 12, 1989
    Date of Patent: July 6, 1993
    Assignee: The Upjohn Company
    Inventors: Leo A. Kominek, Holly J. Wolf, Paula S. Steiert
  • Microbial .DELTA.1-dehydrogenation process using a scavenger of toxic oxygen
    Patent number: 4749649
    Abstract: A process for converting 1,2-saturated 3-keto steroids to 1,2-dehydro steroids which are useful steroid intermediates. The process involves exposing the substrate to steroid-1-dehydrogenase activity in the presence of an added electron carrier and one or more added scavengers of a toxic oxygen species.
    Type: Grant
    Filed: May 16, 1983
    Date of Patent: June 7, 1988
    Assignee: The Upjohn Company
    Inventors: Timothy W. Evans, Leo A. Kominek, Holly J. Wolf, Sheryl L. Henderson
  • .DELTA..sup.1 -dehydrogenation with heat or air-dried B. cyclooxidans
    Patent number: 4704358
    Abstract: Disclosed and claimed is an improved microbial bioconversion to produce 1,2-dehydro steroids from their corresponding 1,2-saturated derivatives.
    Type: Grant
    Filed: April 8, 1985
    Date of Patent: November 3, 1987
    Assignee: The Upjohn Company
    Inventors: Leo A. Kominek, Holly J. Wolf
  • Process for preparing 1,2-dehydro steroids
    Patent number: 4524134
    Abstract: Disclosed and claimed is an improved microbial bioconversion to produce 1,2-dehydro steroids from their corresponding 1,2-saturated derivatives.
    Type: Grant
    Filed: October 25, 1982
    Date of Patent: June 18, 1985
    Assignee: The Upjohn Company
    Inventors: Leo A. Kominek, Holly J. Wolf
  • Process for preparing glyceraldehyde from glycerol with methanol dehydrogenase
    Patent number: 4353987
    Abstract: Novel processes for preparing the useful chemical glyceraldehyde. This compound can be prepared by contacting glycerol with the enzyme methanol dehydrogenase. The enzyme can be in the pure form or crude form. Glycerol also can be converted to glyceraldehyde by contacting it with a methanol dehydrogenase-producing bacterium.
    Type: Grant
    Filed: June 11, 1981
    Date of Patent: October 12, 1982
    Assignee: The Upjohn Company
    Inventor: Holly J. Wolf