Abstract: Provided is a method of serum-free culturing corneal limbal stromal stem cells and inducing them to differentiate and form spheres. Also provide is a medium combination for inducing corneal limbal stromal stem cells to form spheres or differentiate into corneal limbal stromal cells in vitro. The serum-free medium combination used herein can provide sufficient nutrients and a good environment required for cell growth and proliferation, can provide a stable in vitro expansion of corneal limbal stromal stem cells and can ensure that the expanded corneal limbal stromal stem cells keep their stemness and specificity. In addition, a system for inducing them to differentiate into corneal limbal stromal cells is successfully built. It can be used in experimental studies of corneal limbal stromal stem cells, cell therapy of corneal lesions and transplant for corneal injury.

Abstract: Provided is a method of serum-free culturing corneal limbal stromal stem cells and inducing them to differentiate and form spheres. Also provide is a medium combination for inducing corneal limbal stromal stem cells to form spheres or differentiate into corneal limbal stromal cells in vitro. The serum-free medium combination used herein can provide sufficient nutrients and a good environment required for cell growth and proliferation, can provide a stable in vitro expansion of corneal limbal stromal stem cells and can ensure that the expanded corneal limbal stromal stem cells keep their stemness and specificity. In addition, a system for inducing them to differentiate into corneal limbal stromal cells is successfully built. It can be used in experimental studies of corneal limbal stromal stem cells, cell therapy of corneal lesions and transplant for corneal injury.

Abstract: A serum-free culture medium for limbal stem cells and a culture method thereof, wherein the serum-free culture medium includes a basic medium and supplements; wherein the supplements include: human recombinant EGF, insulin, 3,3?,5-triiodo-L-thyronine, hydrocortisone, forskolin, manganese sulfate monohydrate, sodium selenite, sodium metasilicate, ammonium metavanadate, nickel chloride hexahydrate, stannous chloride dihydrate, ethanolamine, O-phosphorylethanolamine, ammonium molybdate tetrahydrate, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid, vitamin C, bovine serum albumin, lipid concentrate and serum substitute.

Abstract: An artificial cornea is fabricated by separately culturing live stromal cells, live corneal endothelial cells (CECs) and live corneal epithelial cells (CEpCs), and 3D bioprinting separate stromal, CEC and CEpC layers to encapsulate the cells into separate hydrogel nanomeshes. The CEC layer is attached to a first side of the stromal layer and the CEpC layer to a second side of the stromal layer to define the artificial cornea.

Abstract: A transformer for reducing the electromagnetic interference (EMI) effect is disclosed. The transformer includes a bobbin; a magnetic core assembly partially sleeved by the bobbin; a first primary winding coiled around the bobbin; a secondary winding coiled on the first primary winding; and a first shielded element disposed between the first primary winding and the secondary winding for disconnecting the EMI transmission from the first primary winding to the secondary winding. The first primary winding includes a first winding portion and a second winding portion, and the first winding portion has larger EMI comparing to the second winding portion. The first winding portion of the first primary winding is adjacently disposed to the magnetic core assembly for shielding the EMI of the first primary winding by using the magnetic core assembly.

Abstract: A transformer for reducing the electromagnetic interference (EMI) effect is disclosed. The transformer includes a bobbin; a magnetic core assembly partially sleeved by the bobbin; a first primary winding coiled around the bobbin; a secondary winding coiled on the first primary winding; and a first shielded element disposed between the first primary winding and the secondary winding for disconnecting the EMI transmission from the first primary winding to the secondary winding. The first primary winding includes a first winding portion and a second winding portion, and the first winding portion has larger EMI comparing to the second winding portion. The first winding portion of the first primary winding is adjacently disposed to the magnetic core assembly for shielding the EMI of the first primary winding by using the magnetic core assembly.

Abstract: A method for eliminating noise interference and acoustic noise by a printed circuit board ground plane layout is disclosed. The method is applied to a circuit system having multiple outputs, wherein the circuit system has a first power converting module, a second power converting module and a printed circuit board. The first power converting module and the second power converting module respectively includes a first ground pin group and a second ground pin group. The method includes the steps of (a) connecting each ground pin of the first ground pin group to a respective solder point and connecting each solder point to a first node, and connecting each ground pin of the second ground pin group to a respective solder point and connecting each solder point to a second node, and (b) connecting the first node and the second node to a common node and connecting the common node to a solder point of a common ground terminal on the printed circuit board ground plane.

Abstract: A method for eliminating noise interference and acoustic noise by a printed circuit board ground plane layout is disclosed. The method is applied to a circuit system having multiple outputs, wherein the circuit system has a first power converting module, a second power converting module and a printed circuit board. The first power converting module and the second power converting module respectively includes a first ground pin group and a second ground pin group. The method includes the steps of (a) connecting each ground pin of the first ground pin group to a respective solder point and connecting each solder point to a first node, and connecting each ground pin of the second ground pin group to a respective solder point and connecting each solder point to a second node, and (b) connecting the first node and the second node to a common node and connecting the common node to a solder point of a common ground terminal on the printed circuit board ground plane.

Abstract: A method of converting a source color map from a source print mode to a destination print mode comprises the steps of performing a constant hue clipping process to preserve ink ratios of destination colors to source colors and performing a channel independent clipping process to preserve ink volume.

Abstract: A method of converting a source color map from a source print mode to a destination print mode comprises the steps of performing a constant hue clipping process to preserve ink ratios of destination colors to source colors and performing a channel independent clipping process to preserve ink volume.