Abstract: A champagne tower-type multi-stage throttle control valve includes a valve body, a valve cover, a throttle sleeve, and a valve core. A sleeve cavity of the throttle sleeve is shaped as a stepped hole with two or more layers. The valve core is shaped as a stepped shaft with two or more layers coaxial with the throttle sleeve. The number of shaft shoulders of the valve core is smaller than or equal to the number of hole shoulders of the sleeve cavity of the throttle sleeve, such that each set of shaft shoulders of the valve core in an axial direction can form a sealing surface fit with corresponding hole shoulders of the throttle sleeve. A flow channel groove is axially or obliquely formed on each of the hole shoulders of the throttle sleeve and/or the shaft shoulders of the valve core.

Abstract: Systems and methods for simulating subterranean regions having multi-scale, complex fracture geometries in a realistic simulation environment, which includes in the modeling process three-dimensional multi-scale rock discontinuities, hydraulic fractures, and heterogenous reservoir properties. Non-intrusive embedded discrete fracture modeling formulations are applied in conjunction with commercial or in-house simulators to efficiently and accurately model subsurface characteristics including three-dimensional geometries having combinations of complex hydraulic fractures and multi-scale rock discontinuities.

Abstract: The present invention discloses a method for establishing a colorectal cancer p73 reporter gene cell line, specifically including: first designing a site-specific sgRNA sequence of a p73 gene and cloning same into a plasmid PX459; integrating a homologous recombination sequence of the p73 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive p73 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line p73 gene and the EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the p73 gene.

Abstract: A champagne tower-type multi-stage throttle control valve includes a valve body, a valve cover, a throttle sleeve, and a valve core. A sleeve cavity of the throttle sleeve is shaped as a stepped hole with two or more layers. The valve core is shaped as a stepped shaft with two or more layers coaxial with the throttle sleeve. The number of shaft shoulders of the valve core is smaller than or equal to the number of hole shoulders of the sleeve cavity of the throttle sleeve, such that each set of shaft shoulders of the valve core in an axial direction can form a sealing surface fit with corresponding hole shoulders of the throttle sleeve. A flow channel groove is axially or obliquely formed on each of the hole shoulders of the throttle sleeve and/or the shaft shoulders of the valve core.

Abstract: The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene.

Abstract: The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene.

Abstract: The present invention discloses a method for establishing a colorectal cancer p73 reporter gene cell line, specifically including: first designing a site-specific sgRNA sequence of a p73 gene and cloning same into a plasmid PX459; integrating a homologous recombination sequence of the p73 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive p73 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line p73 gene and the EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the p73 gene.