Abstract: The present invention provides a molecular identification method for a single dinoflagellate cyst, including: obtaining crushed liquids of the single dinoflagellate cyst; performing a first PCR amplification with the crushed liquids as a template, SEQ ID NO: 1 (GTCCGCCCTCTGGGTG) as a forward primer, and SEQ ID NO: 2 (TCGCAGTAGTSYGTCTTTAAC) as a reverse primer to obtain an amplification product so as to determine the species of the dinoflagellate cyst. If the first amplification product is unsuitable for sequencing due to weak bands by electrophoresis detection, a second (nested) PCR amplification and sequencing is performed with SEQ ID NO: 1 (GTCCGCCCTCTGGGTG) as a forward primer and SEQ ID NO: 2 (TCGCAGTAGTSYGTCTTTAAC) as a reverse primer to determine the species of dinoflagellate.

Abstract: The present invention provides a molecular identification method for single dinoflagellate cysts, comprising: obtaining the crushed liquids of a single dinoflagellate cyst; performing a first PCR amplification with the crushed liquids as templates, one nucleotide sequence of GTCCGCCCTCTGGGTG as a forward primer of an outer primer, and one nucleotide sequence of TCGCAGTAGTSYGTCTTTAAC as a reverse primer of the outer primer to obtain amplification products, and identify the dinoflagellate cyst based on the result of sanger sequencing. If the first amplification product is unsuitable for sequencing due to weaken bands by electrophoresis detection, a second (nested) PCR amplification and sequencing is performed with one nucleotide sequence of GTCCGCCCTCTGGGTG as a forward primer of an inner primer and one nucleotide sequence of TCGCAGTAGTSYGTCTTTAAC as a reverse primer of the inner primer to determine the species of dinoflagellate.