Abstract: The present disclosure discloses a technique for quantitative detection of ?-galactosidase (?-GAL) based on surface-enhanced Raman spectroscopy (SERS), including the following steps: a. taking 180 ?L of each of a 5-bromo-4-chloro-3-indolyl ?-D-galactoside (BCIG) solution, a ?-GAL solution, and dimethylsulfoxide (DMSO); b. preparing a plurality of ?-GAL samples with different activities in advance; c. adding an equal volume of a colloidal gold nanoparticle dropwise to each of the plurality of standard solutions with different activities, and conducting SERS; d. mixing a seawater sample to be tested with BCIG, incubating a resulting mixture to allow a reaction, adding DMSO and a colloidal gold nanoparticle, and directly detecting SERS signals of a product and the DMSO; and e. comparing the SERS signals obtained in step d with the standard curve to obtain an activity of the seawater sample to be tested.

Abstract: The present disclosure belongs to the technical field of clean and efficient mining of deep unconventional or conventional resources, and discloses a pilot-scale supercritical water oxidation oil and hydrogen production system capable of realizing multi-stage heating of organic rock. The system comprises a supercritical water generator, a supercritical water pyrolysis reaction system for organic rock, an oxygen injection system and an oil-gas condensation and collection system, wherein the supercritical water generator mainly comprises a water injection system, a front-section preheating system, a second-stage heating system and a third-stage heating system. The reaction system can carry out a pilot-scale simulation process of supercritical water pyrolysis for organic rock, a multi-stage heating function is realized, the maximum reaction distance is 8 m or more, and the release characteristics of oil-gas products under different reaction distances are explained.

Abstract: The present disclosure belongs to the technical field of clean and efficient mining of deep unconventional or conventional resources, and discloses a pilot-scale supercritical water oxidation oil and hydrogen production system capable of realizing long-distance multi-stage heating of organic rock. The system comprises a supercritical water generator, a supercritical water pyrolysis reaction system for organic rock, an oxygen injection system and an oil-gas condensation and collection system, wherein the supercritical water generator mainly comprises a water injection system, a front-section preheating reaction system, a second-stage heating system and a third-stage heating system. The reaction system can carry out a pilot-scale simulation process of supercritical water pyrolysis for organic rock, a multi-stage heating function is realized, the maximum reaction distance is 8 m or more, and the release characteristics of oil-gas products under different reaction distances are explained.

Abstract: A full-ocean-depth fidelity enzymological measurement device for microbial extracellular enzyme is provided, comprising a pressure-maintaining sampling bottle, pressure-maintaining transfer equipment, a pressure-maintaining enzymological reactor, and heat preservation equipment and enzyme activity detection equipment. The pressure-maintaining enzymological reactor comprises a barrel body, a plug, polytetrafluoroethylene gaskets, an O-ring, a piston, a high-pressure straight-through valve, and a high-pressure connector. The pressure-maintaining enzymological reactor is in a closed barrel body shape and is internally provided with the piston, and the plug and the valve are arranged at each of two ends of the pressure-maintaining enzymological reactor; and the valve is connected to the pressure-maintaining transfer equipment through the high-pressure connector.