Abstract: A dual-vector system for expressing an OTOF protein includes two nucleotide sequences, where a first nucleotide sequence includes two ITR sequences and a gene expression cassette inserted between the ITR sequences; a second nucleotide sequence includes two ITR sequences and a gene expression cassette inserted between the ITR sequences; the gene expression cassette of the first nucleotide sequence includes a promoter, an N-terminal coding sequence of OTOF, an N-terminal coding sequence of intein, and a polyA; and the gene expression cassette of the second nucleotide sequence includes a promoter, a C-terminal coding sequence of Intein, a C-terminal coding sequence of OTOF, and a polyA. The present disclosure further provides an adeno-associated virus packaged by the vector. The vector and the virus can recover hearing of bilateral ears by a unilateral ear administration in the field of deafness gene therapy large gene dual-vector delivery.

Abstract: The invention relates to a block bidding method and system for promoting clean energy consumption based on the power trading platform. The block bidding method includes the following steps: dividing the time slots for clean energy units to participate in power trading; predicting the typical monthly power generation curve of clean energy units; decomposing the signed annual power contract of clean energy units into months; determining the monthly trading declaration curve of clean energy units; determining the monthly trading declaration curve of clean energy units; determining the time-slot declaration price of clean energy units; realizing the trading clearing by the power trading platform in accordance with the principle of “high-low matching”.