Abstract: Disclosed herein include methods, compositions, and kits for use in detecting a target nucleic acid sequence in a sample. Provided include storage-stable lysis buffers. In some embodiments, the lysis buffer comprises: one or more surfactants and magnesium sulfate (MgSO4). In some embodiments, the formation of a precipitate (for example, a complex of Mg+2 and the one or more surfactants) is substantially inhibited for a period of time under at least one storage condition.

Abstract: Disclosed herein include methods, compositions, and kits for use in detecting a target nucleic acid sequence in a sample. Provided include acidic compositions for separating dsDNA. In some embodiments, the acidic compositions comprise a monovalent salt and/or a divalent salt, one or more surfactants, and an acidic agent. In some embodiments, the pH of the acidic composition is less than 4. Also provided herein are reagent compositions comprising a buffering agent and one or more amplification agents.

Abstract: Disclosed herein include methods, kits, and reaction mixtures suitable for use in monitoring an amplification reaction. Some embodiments provide a first and a second quality control primer capable of hybridizing to each other and each comprising a quenchable label or a quencher.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: A method for evaluating the health status of a petrochemical atmospheric oil storage tank using data from multiple sources. The health status of an atmospheric oil storage tank is influenced by multiple factors, and is evaluated by: acquiring corresponding sensor data and comprehensively considering the sensor data along with basic data of the oil storage tank, and selecting from a dynamic monitoring parameter-based health status and a basic health status of the oil storage tank, the one having a greater severity level, so as to determine the final health status of the oil storage tank. The method is used to conduct a comprehensive scientific assessment of the health status of an oil storage tank, and improves the use safety of the oil storage tank.

Abstract: This invention relates to methods and compositions for detecting the presence or absence of a Respiratory Syncytial Virus target nucleic acid in a biological sample using isothermal nucleic acid amplification.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: This invention relates to compositions, methods and kits for detecting the presence or absence of a bacterial species in a biological sample using isothermal nucleic acid amplification.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: This invention relates to compositions, methods and kits for detecting the presence or absence of a bacterial species in a biological sample using isothermal nucleic acid amplification.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide.

Abstract: A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Abstract: The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.