Abstract: A cell model for in vitro evaluation of compound-induced skin sensitization and a constructing method therefor. The method for constructing the cell model comprises the steps of: designing and constructing an sgRNA expression vector based on CRISPR/Cas9 vector system; designing and constructing a homologous recombinant vector capable of knocking a reporter gene linked to a self-cleaving peptide sequence into a specific site of the expression frame of the HMOX1 gene; co-transfecting the homologous recombinant vector, an hCas9 plasmid and the sgRNA expression vector into a cell, and carrying out monoclonal expansion to obtain the cell model. The present invention obtains a HaCaT cell model in which a luciferase gene is knocked in before the stop codon of the HMOX1 gene by combination of CRISPR/CAS9 and a monoclonal cell technique. The cell model realizes synchronous expression of the luciferase gene and the HMOX1 gene, thereby effectively distinguishing sensitizing compounds from non-sensitizing compounds.

Abstract: Seawater fluidized ice manufacturing equipment is disclosed, comprising a compressor; a condenser, connected with the compressor, the condenser being internally provided with a condensing agent; a heat exchanger, connected with the condenser, the heat exchanger being provided with a hydrophobic material coating; and an ultrasonic generator, connected with the heat exchanger. A seawater fluidized ice manufacturing method is also disclosed, comprising the following steps: compressing low-temperature and low-pressure condensing agent vapor in an ice making barrel into high-temperature and high-pressure condensing agent vapor by the compressor; driving the seawater to enter the ice making barrel and the liquefied condensing agent into a cooling pipe, condensing the seawater into fluidized ice, wherein when the seawater is condensed in the ice making barrel, the ultrasonic generator generates ultrasonic waves to drive the seawater to crystallize into fluidized ice.

Abstract: Provided in the present invention are a di-substituted maleic amide linker conjugated to an antibody and a preparation method and use thereof. In particular, the present invention conjugates a strongly cytotoxic active substance to a biomacromolecule through a class of new linkers. The class of linkers can selectively act simultaneously with disulphide chains, so as to greatly improve the substance homogeneity of a conjugate. The conjugate prepared by the linker of the present invention has a high inhibitory activity on a cell strain expressing the corresponding antigen. Also provided is a method for preparing the above-mentioned conjugate and the use.

Abstract: Web processing method and apparatus (30, 300) is provided for high speed, extremely accurate die cutting or lamination operations. Processing station (32,300) includes a vacuum hold down plate (32,308) which receives and holds an image bearing incremental segment of the web. In feed and out feed tension on the web is released while a segment of the web is held by the hold down plate. The hold down plate with a segment of the web thereon is selectively shifted about X, Y, and ? axes as required to bring the image on the web segment into alignment with a web processing component at the processing station.

Abstract: Process control systems may include one or more diagnostic systems and/or processes. In particular implementations, a diagnostic system and process for a field instrument may include the ability to divide a signal input range into a plurality of zones with respect to an input axis or a time axis, measure input response characteristics for a zone with the field instrument, and store measurement data points for the zone in memory of the field instrument. The diagnostic system and process may also include the ability to transmit the measurement data points for the zone to a host application and, while transmitting the measurement data points for the zone, measure input response characteristics for a second zone with the field instrument.

Abstract: The present invention provides an off-line diagnosis system whereby the measurement resolution (number of measurement points) can be increased in order to obtain detailed input-output characteristics, and step response characteristics can be obtained at a sufficient frequency and more efficiently, without expanding the physically limited memory capacity of a field instrument or host application.

Abstract: Process control systems may include one or more diagnostic systems and/or processes. In particular implementations, a diagnostic system and process for a field instrument may include the ability to divide a signal input range into a plurality of zones with respect to an input axis or a time axis, measure input response characteristics for a zone with the field instrument, and store measurement data points for the zone in memory of the field instrument. The diagnostic system and process may also include the ability to transmit the measurement data points for the zone to a host application and, while transmitting the measurement data points for the zone, measure input response characteristics for a second zone with the field instrument.

Abstract: Web processing apparatus (30, 300) is provided for high speed, extremely accurate die cutting or lamination operations. Processing station (32, 300) includes a Vacuum hold down plate (32, 308) which receives and holds an image bearing incremental segment of the web. In feed and out feed tension on the web is released while a segment of the web is held by the hold down plate. The hold down plate with a segment of the web thereon is selectively shifted about X, Y and ? axis as required to bring the image on the web segment into alignment with a web processing component at the processing station.

Abstract: High speed, extremely accurate web or sheet-fed segment die cutting or lamination apparatus (30, 300) has a processing station (32, 300), which receives a sheet or web segment, and is provided with a vacuum hold-down plate (142, 306) for holding initially fed segments (38). The plate is shiftable as necessary along orthogonal X-Y axes in the plane of the segment (38), and/or is rotatable about a &thgr; axis transverse to the segment plane. Plate movement is effected by a series of aligned, translatable eccentric drive units (178-182, 346-350). Segments (38) carry positioning fiducials (44) that are compared with fixed reference indicia (250, 252) in the station (32, 300). The comparison data is used by a controller (254) to generate the plate movement information used in simultaneous operation of the associated plate drive units (178-182, 346-350).

Abstract: A die cutting press (10) having a base platen (12), a bolster (16) shiftably and rotatably mounted on the base platen, and a die unit (22) mounted on the bolster for movement therewith is provided for precision cutting of individual part-defining areas (30, 230) from longitudinal and laterally-arranged rows of part areas of a relatively thin substrate (32, 232) that is susceptible to deformation and warpage during its manufacture and wherein registration indicia (236, 238) is provided in association with each of the part-defining areas.

Abstract: Web or sheet-fed segment processing apparatus (30, 300) is provided for high speed, extremely accurate operations such as die cutting or lamination. The apparatus (30, 300) includes a processing station (32, 300) adapted to receive a segment (38) forming a part of a continuous web (100, 102) or as a discrete sheet. The station (32, 300) includes a vacuum hold-down plate (142, 306) for holding initially fed segments (38); the hold-down plate (32, 308) is shiftable as necessary along orthogonal X-Y axes in the plane of the segment (38), and/or &thgr; rotation about a rotational axis transverse to the segment plane, such movement being effected by a series of aligned, translatable eccentric drive units (178-182, 346-350) coupled with plate (142, 306). Preferably, the segments (38) carry positioning fiducials (44) and which are compared with fixed reference indicia (250, 252) in the station (32, 300).

Abstract: Web or sheet-fed segment processing apparatus (30, 300) is provided for high speed, extremely accurate operations such as die cutting or lamination. The apparatus (30, 300) includes a processing station (32, 300) adapted to receive a segment (38) forming a part of a continuous web (100, 102) or as a discrete sheet. The station (32, 300) includes a vacuum hold-down plate (142, 306) for holding initially fed segments (38); the hold-down plate (32,308) is shiftable as necessary along orthogonal X-Y axes in the plane of the segment (38), and/or &thgr; rotation about a rotational axis transverse to the segment plane, such movement being effected by a series of aligned, translatable eccentric drive units (178-182,346-350) coupled with plate (142,306). Preferably, the segments (38) carry positioning fiducials (44) and which are compared with fixed reference indicia (250, 252) in the station (32, 300).