Abstract: The invention relates to the allergenic latex protein Hev b 4. The full cDNA sequence of the smaller protein components of Hev b 4 is disclosed, together with the amino acid sequence of those components and the synthesis of a recombinant protein using the aforesaid DNA sequence or a synthetic peptide derived from the amino acid sequence. The use of the Hev b 4 protein complex and of an antibody against that protein is described in immunoassays and in immunotherapy for the relief of allergy or allergy symptoms.

Abstract: This invention relates to allergenic proteins of natural rubber latex in substantially purified form, their production and their use, together with monoclonal antibodies developed against those allergenic proteins, in assays for the qualitative and quantitative determination of the levels of the allergenic proteins in natural rubber latex or in products made from latex. Assays for identifying and/or quantitating antibodies in blood or blood products that mediate the occurrence of an allergic reaction induced by natural rubber latex are also provided, together with in vivo and in vitro diagnostic tests for detecting hypersensitivity to natural rubber latex and which involve use of the aforesaid allergenic proteins. The invention also provides for the use of the aforesaid allergens as de-sensitising agents in the treatment of latex protein allergy. There is still further provided a method for removing allergenic proteins from latex products.