Abstract: A detection chip and a manufacturing method thereof, and a detection method are provided. The detection chip includes: a base substrate, and a detection layer, provided on the base substrate and including a plurality of detection holes. An inner wall of each of at least part of detection holes among the plurality of detection holes is hydrophilic, and a contact angle of the inner wall is within 30 degrees.

Abstract: A detection chip and a preparation method thereof, the detection chip may include a base substrate and a detection layer, the detection layer is provided on the base substrate and include a plurality of detection holes, each of at least a portion of the plurality of detection holes has a hole wall provided with a hydrophilic layer, and a contact angle of the hydrophilic layer is within 20 degrees. The detection chip can implement high-throughput, crosstalk does not easily occur between adjacent detection holes, which, thus, may improve detection accuracy.

Abstract: The present disclosure provides a microfluidic chip including a plurality of microcavities, at least two of the plurality of microcavities have different volumes.