Abstract: This invention seeks to provide means by which transgenic plants harbour within their genome a chimaeric gene which when expressed produces a protein having a cytotoxic effect. Cytotoxicity is achieved by way of a protein or part thereof capable of blocking cellular protein synthesis, such as a maize ribosome inactivating protein or part thereof. The chimaeric gene may comprise a promoter operably linked to a sequence encoding a protein capable of blocking cellular protein synthesis such that the promoter is inducible at and/or adjacent to a target site.

Abstract: The invention is the identification of double stranded RNA nematcides to nematode genes and a screening assay for the identification of agents, typically dsRNA's, with nematicidal activity

Abstract: A method is provided for protecting a plant from nematodes and diseases associated with nematode invasion, which method comprises the step of inoculating said plant with a nematode-inhibiting strain of P. cepacia capable of colonizing said plant. The P. cepacia strain to be used should be non-pathogenic to said plant. Root inoculation of a plant with a nematode-inhibiting P. cepacia results in protection of the plant roots from nematode invasion. Foliar inoculation of the plant with these P. cepacia strains results in protection of the above-ground portions of the plant from nematode attack. Direct or indirect inoculation of seeds with nematode-inhibitory strains of P. cepacia strains can establish colonization of roots, stems and leaves of plants, and thus result in the protection of both roots and above-ground plant parts. The inoculum can be applied, for example, in the vicinity of a seed or young plant or it can be directly applied to a seed or to the above-ground parts of a young plant.