Abstract: The invention relates to the directed evolution of CYP52A12 gene and the use thereof for the production of a dicarboxylic acid. In particular, it relates to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. In particular, the invention relates to a method of preparing a long chain dicarboxylic acid producing strain by directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. By directed evolution of CYP52A12 gene, one strain which has a base mutation at the promoter region of said gene and is capable of producing a long chain dicarboxylic acid under an acidic condition in a shortened fermentation time is screened out in the invention.

Abstract: One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof.

Abstract: Provided herein is a genetically modified host cell comprising a heterologous nucleic acid encoding a biofilm dispersal polypeptide that decreases intracellular c-di-GMP levels and enhances the production of lysine and lysine derivatives. Further provided are methods of generating such cell and producing lysine and lysine derivatives using the genetically modified host cell.

Abstract: Provided are microorganisms genetically modified to overexpress porin polypeptides to enhance the production of lysine and lysine derivatives by the microorganism. Also provided are methods of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.

Abstract: Provided are lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 and/or fragments thereof. The mutants or fragments have at least 95% sequence identity with SEQ ID NO: 2. Also provided are DNA polynucleotides encoding said lysine decarboxylases, expression vector comprising the DNA polynucleotides, transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.

Abstract: The present invention provides a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA), and a polynucleotide encoding the polypeptide. The present invention provides a first expression plasmid vector comprising one or more first polynucleotides encoding a tetracycline efflux pump, one or more second polynucleotides independently selected from the group consisting of a third polynucleotides encoding a lysine decarboxylase polypeptide and a fourth polynucleotides encoding a lysine biosynthesis polypeptide, and a transformant comprising the expression plasmid vector. The present invention provides a host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of the host cell. The present invention also provides a method for producing a lysine of a cadaverine using the transformant and/or the host cell.

Abstract: The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.

Abstract: The present invention relates to methods and compositions for increasing production of 3-methyl-2-butenol in a de novo synthetic pathway in a genetically modified host cell using isopentenyl disphosphate (IPP) as a substrate.

Abstract: A method for integrating interactions in an address book application that includes managing a plurality of contacts of an electronic address book, identifying a contact with a relationship to an interaction service, collecting dynamic information corresponding to the identified contact, and facilitating an interaction using the dynamic information.

Abstract: A first-in-first-out memory may have first and second memory banks. A write controller may write data into the first and second memory banks. In performing write operations, the write controller may determine whether to write the data into the first bank or the second bank by evaluating a first bank empty flag and a second bank empty flag. When transitioning between writing in the first bank and the second bank, the write controller may latch a write address value indicative of the last location at which valid data was written in a given bank. A read controller may read data from the first and second memory bank. The read controller may determine when to transition between reading in the first bank and reading in the second bank by comparing a current read address to the latched write address value.

Abstract: Integrated circuits with memory elements are provided. Data may be loaded into the memory elements using write driver circuitry. The write driver circuitry may be provided with a fixed positive power supply voltage and an time-varying ground power supply voltage that is less than the positive power supply voltage. The time-varying ground power supply voltage may be generated using programmable power supply circuitry. The programmable power supply circuitry may include a pulse generation circuit and a configurable capacitive circuit. The pulse generation circuit may output a pulse signal to the capacitive circuit. In response to receiving the pulse signal, the capacitive circuit may push the time-varying ground power supply voltage to a negative value. The time-varying ground power supply voltage may be driven below zero volts for at least a portion of a write cycle to help improve write margins and increase memory yield.

Abstract: The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase.

Abstract: An integrated circuit with memory elements is provided. The memory elements may be single-port memory cells that are used to provide multiport memory functionality. The integrated circuit may include an arbitration circuit operable to receive memory access requests from at least first and second request generators. The arbitration circuit may be configured to operate in a synchronous mode and an asynchronous mode. The arbitration circuit operating in the synchronous mode may perform port selection based on a predetermined logic table. The arbitration circuit operating in the asynchronous mode may execute a memory request as soon as it is received by the arbitration circuit. Requests received while a current memory access is being performed may be put on hold until the current memory access has been completed.

Abstract: Integrated circuits with memory elements are provided. A memory element may include a storage circuit coupled to data lines through access transistors. The access transistors may have gates that are controlled by an address signal. The address signal may be asserted during read/write operations to turn on the access transistors so that read/write data can be passed through the access transistors. The voltage level to which the address signal is raised during read/write operations may be adjusted using programmable voltage biasing circuitry. A number of integrated circuits may be tested during device characterization procedures to determine the amount by which the address signal should be adjusted using the programmable voltage biasing circuit so that the memory elements in the integrated circuits satisfy design criteria.

Abstract: An integrated circuit with memory elements is provided. The memory elements may be single-port memory cells that are used to provide multiport memory functionality. The integrated circuit may include an arbitration circuit operable to receive memory access requests from at least first and second request generators. The arbitration circuit may be configured to operate in a synchronous mode and an asynchronous mode. The arbitration circuit operating in the synchronous mode may perform port selection based on a predetermined logic table. The arbitration circuit operating in the asynchronous mode may execute a memory request as soon as it is received by the arbitration circuit. Requests received while a current memory access is being performed may be put on hold until the current memory access has been completed.

Abstract: A method for integrating interactions in an address book application that includes managing a plurality of contacts of an electronic address book, identifying a contact with a relationship to an interaction service, collecting dynamic information corresponding to the identified contact, and facilitating an interaction using the dynamic information.

Abstract: Integrated circuits such as programmable logic device integrated circuits are provided that have memory arrays that may be configured for true dual port operation or simple dual port operation. The memory arrays include memory cells arranged in rows and columns and associated row address lines and data lines. Sense amplifiers and write drivers are used for reading and writing data. Precharge drivers are used to precharge the data lines prior to read operations. Configurable multiplexer circuitry in the array has read paths through which data is provided to the sense amplifiers from the memory cells. The multiplexer circuitry has write paths through which data from the write drivers is written into the memory cells. The read paths and the write paths contain no more than a single pass gate each. Each precharge driver may be connected to a respective one of the data lines with no intervening pass gates.

Abstract: A protein microarray element comprising: a) a support; b) a gelatin layer containing functional groups capable of binding biological probes; and interposed between the support and the gelatin layer and c) an adhesive interlayer layer capable of maintaining contact with the support and with the gelatin layer.