Abstract: This disclosure relates to placental membrane preparations and the methods of preparing and using thereof. In some embodiments, the disclosure relates to a placental membrane preparation. In some embodiments, the disclosure relates to methods of producing a placental membrane preparation. In some embodiments, the disclosure relates to methods of treating cartilage using placental membrane preparations.

Abstract: The present invention relates to the isolation, propagation and use of prenatal stem cells. The cells are CD105 negative. In certain embodiments the cells are also SSEA-4 positive or negative, and c-kit negative. The cells may be isolated from a prenatal sample, for instance third trimester amniotic fluid or placental membrane. The cells may be combined with a biologically compatible solution or a biologically compatible matrix and utilized to treat a human subject. The invention also contemplated enriched populations of prenatal stem cells. The enriched cell population may be propagated in the appropriate culture medium and the resulting progeny cells utilized therapeutic applications.

Abstract: Preparations containing hepatocyte growth factor (HGF) and hyaluronic acid (HA) and methods of making and using same. The HGF and HA preparations can be prepared together in solution as an injectable fluid without gelatinization, or impregnated within a porous hydrophilic matrix material with, or without, cross-linking of the HA with the matrix material. The preparations can be used as a dermal filler or to generate and promote healing of cartilage, vertebral discs, connective tissues such as tendons and ligaments and bone in vivo.

Abstract: A method and devices for placing spinal implants including placing the implants completely within a spaced defined between adjacent vertebral bodies where the implants are supported by the cortical bone of the vertebral bodies. An insertion instrument places the implants in pairs with a variable-sized space placed in between. The implants are made of a biocompatible material and are particularly suited for anterior lumbar interbody fusion surgery. The spinal implants used to facilitate spinal fusion, correct deformities, stabilize and strengthen the spine.

Abstract: Preparations derived from placental materials and methods of making and using same, the preparations including a first preparation composed of placental membranes digested in collagenase, a second preparation composed of multipotent cells derived from the collagenase digested placental membranes and that are grown in adherent culture beyond confluence and a third preparation composed of ground placental membranes re-suspended in a fluid containing hyaluronic acid. The preparations can be used for regenerating damaged or defective tissue including connective tissue, nerve tissue, muscle tissue, skin tissue, cartilage tissue and bone tissue. The preparations can also be used as dermal fillers in cosmetic and plastic surgery applications.