Abstract: A handheld ultrasound detection apparatus includes a portable housing with a flexible tube that extends from the housing and includes a sound cup and an ultrasound receiver for converting received ultrasound energy into an electronic signal. A gain/active filter includes four amplifiers each having an associated gain range. The apparatus is programmed based on a size of the electronic signal, to direct the electronic signal along a linear scale through one or more amplifiers and apply an appropriate gain to the signal, and to generate a percentage value corresponding to the gain and with respect to the linear scale. In a manual mode, data is generated corresponding to a particular gain range and a percentage value from 0-100% with respect to the particular gain range, while during an automatic mode data is generated corresponding to a percentage value from 0-400% with respect to a collective gain range.

Abstract: A system and method for comparative analysis of sound data obtained from client equipment includes a portable sound detection device with a transducer to collect sounds from equipment, convert the collected sounds into analog signals, and generate digital data files including sound data representative of the collected sounds. The data files are stored in a web-based database and remote server-based program modules aggregate data associated with the data files with previously stored and related data across a plurality of predetermined data parameters. The program executes or enables identification of outlying data points with respect to data points from the sound data, and the comparison of sound data with baseline data determined the equipment type, the location and the aggregated data. The program then executes or enables identification of data points from the sound data representative of an equipment failure, or an imminent failure thereof.

Abstract: Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions.

Abstract: Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions.