Patents by Inventor Hrair Kirakossian
Hrair Kirakossian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11266988Abstract: The invention, is directed to devices and methods for low cost and convenient separation, of plasma from whole blood. In some embodiments, devices of the invention comprise an integrated collection of channels and chambers m a body that permit acquisition of a blood sample by capillary action, centrifugal separation of cells from plasma, and manual dispensing of purified plasma by simple pinching of a bellows chamber to force air into plasma-holding channels which thereby expels a predetermined volume of the purified plasma.Type: GrantFiled: March 14, 2018Date of Patent: March 8, 2022Inventors: Hrair Kirakossian, Ming Tan
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Publication number: 20200030792Abstract: The invention, is directed to devices and methods for low cost and convenient separation, of plasma from whole blood. In some embodiments, devices of the invention comprise an integrated collection of channels and chambers m a body that permit acquisition of a blood sample by capillary action, centrifugal separation of cells from plasma, and manual dispensing of purified plasma by simple pinching of a bellows chamber to force air into plasma-holding channels which thereby expels a predetermined volume of the purified plasma.Type: ApplicationFiled: March 14, 2018Publication date: January 30, 2020Inventors: Hrair Kirakossian, Ming Tan
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Patent number: 9939447Abstract: Compositions, kits, and methods are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. The compositions can include a first reagent including a cleavage-inducing moiety and a first binding agent for a binding site that includes a post-translational modification of a target polypeptide.Type: GrantFiled: August 14, 2015Date of Patent: April 10, 2018Assignee: Monogram Biosciences, Inc.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracy J. Matray, Vincent S. Hernandez
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Patent number: 9535065Abstract: The present disclosure provides cytometric methods for the detection of rare target cells in a sample. In certain aspects, the methods and compositions may facilitate the detection of rare target cells, such as circulating tumor cells (CTCs), in a biological sample such as blood. Aspects of the methods include contacting the sample with first and second binding members that specifically bind to a marker of the rare target cell, and cytometrically assaying the sample for the presence of cells comprising bound first and second binding members to detect the rare target cell in the sample. Also provided are systems, compositions, and kits for practicing the subject methods.Type: GrantFiled: September 6, 2012Date of Patent: January 3, 2017Assignee: Becton, Dickinson and CompanyInventors: Hrair Kirakossian, Edward Goldberg, Diether Recktenwald
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Publication number: 20160069893Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide.Type: ApplicationFiled: August 14, 2015Publication date: March 10, 2016Applicant: MONOGRAM BIOSCIENCES, INC.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracy J. Matray, Vincent S. Hernandez
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Patent number: 9110075Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.Type: GrantFiled: October 4, 2011Date of Patent: August 18, 2015Assignee: Monogram Biosciences, Inc.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracy J. Matray, Vincent S. Hernandez
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Patent number: 8951784Abstract: A cell culture bioreactor having a culture chamber for cell growth, a culture medium reservoir mounted externally to the culture chamber, a fluid circulation mechanism for moving culture medium from the reservoir through the culture chamber, and an aerating mechanism for introducing air into and removing air from the culture chamber is provided. The cell culture bioreactor comprises a manifold mechanism mounted at the top end of the culture chamber, a fluid circulation mechanism fluidly connected to the culture medium reservoir, for receiving culture medium from the culture medium reservoir and distributing culture medium into the culture chamber.Type: GrantFiled: December 13, 2006Date of Patent: February 10, 2015Assignee: Sepragen CorporationInventors: Dennis Richard Gould, Hrair Kirakossian
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Publication number: 20140242610Abstract: The present disclosure provides cytometric methods for the detection of rare target cells in a sample. In certain aspects, the methods and compositions may facilitate the detection of rare target cells, such as circulating tumor cells (CTCs), in a biological sample such as blood. Aspects of the methods include contacting the sample with first and second binding members that specifically bind to a marker of the rare target cell, and cytometrically assaying the sample for the presence of cells comprising bound first and second binding members to detect the rare target cell in the sample. Also provided are systems, compositions, and kits for practicing the subject methods.Type: ApplicationFiled: September 6, 2012Publication date: August 28, 2014Applicant: BECTON, DICKINSON AND COMPANYInventors: Hrair Kirakossian, Edward Goldberg, Diether Recktenwald
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Patent number: 8679764Abstract: The invention provides a low-cost method for identifying and/or measuring a subpopulation of cells in a biological sample, such as a subpopulation of CD4-positive cells in a whole blood sample. In accordance with the invention, one or more cell surface markers are identified on a subpopulation of interest, which collectively are present in higher numbers on the cells of the subpopulation than on cells not in the subpopulation of interest. A probe comprising a binding compound and a density label are combined with the sample and the resulting mixture is centrifuged or sedimented through a density medium in a capillary. Labeled cells of interest pass through the density medium and stack at the bottom of the capillary where they may be visually detected and quantified by the height of the stack.Type: GrantFiled: March 3, 2009Date of Patent: March 25, 2014Assignee: Becton, Dickinson and CompanyInventor: Hrair Kirakossian
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Patent number: 8628933Abstract: The invention relates to a kit for a homogenous detection method for the quantitative or qualitative detection of an analyte in an assay and adequate reagents therefor, particularly a homogenous binding test. According to the invention, an analyte-specific binding partner R1 comprises more than one specific binding point for a specific binding partner X that is associated with a component of a signal-forming system while a second analyte-specific binding partner R2 comprises more than one specific binding point for a specific binding partner Y which is also associated with a component of a signal-forming system.Type: GrantFiled: January 23, 2013Date of Patent: January 14, 2014Assignee: Siemens Healthcare Diagnostics Products GmbHInventors: Carsten Schelp, Michael Trier, Hrair Kirakossian
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Patent number: 8399209Abstract: The invention relates to methods for the quantitative or qualitative detection of an analyte in an assay and adequate reagents therefor, particularly a homogeneous binding test. According to the invention, an analyte-specific binding partner R1 comprises more than one specific binding point for a specific binding partner X that is associated with a component of a signal-forming system while a second analyte-specific binding partner R2 comprises more than one specific binding point for a specific binding partner Y which is also associated with a component of a signal-forming system.Type: GrantFiled: May 31, 2006Date of Patent: March 19, 2013Assignee: Siemens Healthcare Diagnostics Products GmbHInventors: Carsten Schelp, Michael Trier, Hrair Kirakossian
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Publication number: 20120088307Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.Type: ApplicationFiled: October 4, 2011Publication date: April 12, 2012Applicant: MONOGRAM BIOSCIENCES, INC.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracy J. Matray, Vincent S. Hernandez
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Patent number: 7927561Abstract: The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4+T cells in a whole blood sample.Type: GrantFiled: December 15, 2008Date of Patent: April 19, 2011Assignee: Becton, Dickinson and CompanyInventors: Hrair Kirakossian, Liping Yu, Douglas A. Petry
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Publication number: 20100068754Abstract: The invention provides a low-cost method for identifying and/or measuring a subpopulation of cells in a biological sample, such as a subpopulation of CD4-positive cells in a whole blood sample. In accordance with the invention, one or more cell surface markers are identified on a subpopulation of interest, which collectively are present in higher numbers on the cells of the subpopulation than on cells not in the subpopulation of interest. A probe comprising a binding compound and a density label are combined with the sample and the resulting mixture is centrifuged or sedimented through a density medium in a capillary. Labeled cells of interest pass through the density medium and stack at the bottom of the capillary where they may be visually detected and quantified by the height of the stack.Type: ApplicationFiled: March 3, 2009Publication date: March 18, 2010Inventor: Hrair Kirakossian
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Publication number: 20090325192Abstract: The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4+T cells in a whole blood sample.Type: ApplicationFiled: December 15, 2008Publication date: December 31, 2009Inventors: Hrair Kirakossian, Liping Yu, Douglas A. Petry
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Patent number: 7537938Abstract: A method is provided for detecting biomarkers in rare circulating cells by forming an enriched population of cells immunomagnetically followed by biomarker detection using binding compounds having releasable molecular tags. Preferably, biomarkers comprising one or more protein-protein complexes are detected in circulating cancer cells metastasized from a solid tumor. In the presence of biomarkers, the molecular tags of the binding compounds are released and separated from the assay mixture for detection and/or quantification.Type: GrantFiled: January 26, 2004Date of Patent: May 26, 2009Assignee: Monogram Biosciences, Inc.Inventors: Hrair Kirakossian, Thomas Klopack, Sharat Singh
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Publication number: 20080311674Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide.Type: ApplicationFiled: August 10, 2007Publication date: December 18, 2008Applicant: Monogram Biosciences, Inc.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracey J. Matray, Vincent S. Hernandez
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Patent number: 7341362Abstract: A photoactivation apparatus (200) separately delivers light to a plurality of wells of a multiwell plate (110). The device-includes a plurality of light emitting diodes (210) attached to a first surface of a substrate (220) such that when the photoactivation apparatus is positioned on the multiwell plate each of the light emitting diodes delivers light to one corresponding well. The light emitting diodes (210) may extend at least partially into the wells of the multiwell plate. A heat sink (250) and fan may be provided on the device to dissipate heat generated by the light emitting diodes. The components are preferably enclosed in a conveniently-sized housing (270). The housing may include one or more grips and air vents. The apparatus includes an interlocking lip (230) defining a recess which is sized to receive at least a portion of the multiwell plate. A power supply connected to the apparatus and configured to provide an equal voltage across each of the light emitting diodes.Type: GrantFiled: December 18, 2002Date of Patent: March 11, 2008Assignee: Monogram Biosciences, Inc.Inventors: Torleif Bjornson, Hrair Kirakossian, Albert W. Brown, Jr., William Wevers, Hossein Salimi-Moosavi
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Patent number: 7255999Abstract: Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide.Type: GrantFiled: May 21, 2002Date of Patent: August 14, 2007Assignee: Monogram Biosciences, Inc.Inventors: Sharat Singh, Hossein Salimi-Moosavi, Syed Hasan Tahir, Gerald J. Wallweber, Hrair Kirakossian, Tracy J. Matray, Vincent S. Hernandez
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Publication number: 20070134790Abstract: A cell culture bioreactor having a culture chamber for cell growth, a culture medium reservoir mounted externally to the culture chamber, a fluid circulation mechanism for moving culture medium from the reservoir through the culture chamber, and an aerating mechanism for introducing air into and removing air from the culture chamber is provided. The cell culture bioreactor comprises a manifold mechanism mounted at the top end of the culture chamber, a fluid circulation mechanism fluidly connected to the culture medium reservoir, for receiving culture medium from the culture medium reservoir and distributing culture medium into the culture chamber.Type: ApplicationFiled: December 13, 2006Publication date: June 14, 2007Inventors: Dennis Gould, Hrair Kirakossian