Abstract: A p-aminobenzoic acid-producing microorganism is provided. The p-aminobenzoic acid-producing microorganism is obtained by a method for preparing a p-aminobenzoic acid-producing microorganism. The method for preparing a p-aminobenzoic acid-producing microorganism includes (a) performing an acclimation process on a source microorganism with at least one sulfonamide antibiotic to obtain at least one acclimatized microorganism and (b) screening out at least one p-aminobenzoic acid-producing microorganism from the at least one acclimatized microorganism, wherein the at least one p-aminobenzoic acid-producing microorganism has a higher p-aminobenzoic acid titer than the source microorganism.

Abstract: A dye for a material with amide functional groups and a dyeing method of using the same are provided. The dye includes a compound represented by Formula (I) and a solvent. Based on the total weight of the dye of 100 wt %, a content of the compound represented by Formula (I) is 40 wt % to 95 wt %. In Chemical Formula (I), R1, R2 and R3 are each independently H, —OH or —COOH, wherein at least one of R1, R2 and R3 is —OH or —COOH.

Abstract: A dyeing method includes immersing a fiber into a dye for dyeing the fiber, in which the dye includes indigo and indirubin, and the indigo and the indirubin have a weight ratio of 20:1 to 80:1. The indigo in the dye has a concentration of 0.1% o.w.f. to 5% o.w.f. The dyed fiber may simultaneously have high luminance, high color saturation, high strength of colorization, and sufficient colorfastness.

Abstract: The disclosure provides a dyeing method using bio-dye. A first compound containing an X group is reacted with a second compound containing a Y group to form a third compound, in which the X group is selected from the group consisting of an isocyanate group, a carbodiimide group, an aziridinyl group and an epoxy group, the Y group is selected from the group consisting of a hydroxyl group and an amine group, and the second compound is an amine compound and contains two or more Y groups. Cationization of the third compound is carried out with a fourth compound containing a carboxyl group to form a cationic modification agent. A cationic modification treatment is performed on a fiber material by using the cationic modification agent to form a cationized fiber material. Thereafter, a dyeing treatment is performed on the cationized fiber material through a dye solution containing bio-dye to color the cationized fiber material.

Abstract: A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.

Abstract: A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.

Abstract: A genetic engineered bacteria without or comprising a plurality of important metabolic enzyme related genes is provided. When the by-product or waste of fruit and vegetable is used as the culture medium, a large quantity of succinic acid or lactic acid can be produced via fermentation. A method of producing succinic acid and lactic acid using the genetic engineered bacteria is also provided.

Abstract: A genetic engineered bacteria without or comprising a plurality of important metabolic enzyme related genes is provided. When the by-product or waste of fruit and vegetable is used as the culture medium, a large quantity of succinic acid or lactic acid can be produced via fermentation. A method of producing succinic acid and lactic acid using the genetic engineered bacteria is also provided.

Abstract: Described herein is a fusion polypeptide containing an aconitase and a cis-aconitate decarboxylase. Also described are a genetically modified cell expressing the fusion polypeptide and a method of using the cell to produce itaconate.

Abstract: Described herein is a fusion polypeptide containing an aconitase and a cis-aconitate decarboxylase. Also described are a genetically modified cell expressing the fusion polypeptide and a method of using the cell to produce itaconate.