Abstract: A method for three-dimensional cell culture using a cell growth promoting colloid and a mass production of high-concentration extracellular composition using the same are provided. The method for three-dimensional cell culture using the cell growth promoting colloid includes: providing a culture colloid to mix with cells to embed the cells therein; performing a crosslinking treatment to the culture colloid embedding the cells, so that the culture colloid that is crosslinked has a three-dimensional structure and the cells are suspended in the culture colloid that is crosslinked; and performing cell culture.

Abstract: A method for manufacturing a sterile bio-ink includes providing an extracellular matrix composition, and the extracellular matrix composition being in a liquid state; adding an animal-based gel and a plant-based gel that are freeze-dried, re-dissolved, and filtered to the extracellular matrix composition, and mixing the extracellular matrix composition, the animal-based gel, and the plant-based gel to obtain a gel mixture; and centrifuging and degassing the gel mixture to obtain the bio-ink.

Abstract: A method for manufacturing an extracellular matrix composition includes providing a gel, carrying out a crosslinking treatment, carrying out a single type cell culture, carrying out a decrosslinking treatment, and carrying out an extraction treatment. The crosslinking treatment includes adding a crosslinking agent to the gel to obtain a crosslinked gel. The cell culture includes implanting cells of a singular type on the crosslinked gel and incubating the cells by adding a culture solution. The decrosslinking treatment includes adding a decrosslinking agent to the crosslinked gel to obtain a decrosslinked mixture that contains an extracellular matrix. The extraction treatment includes filtering the decrosslinked mixture to obtain the extracellular matrix composition that is in a liquid state.

Abstract: A mesenchymal stem cell culture product and a method for preparing the same are provided. The method for preparing the mesenchymal stem cell culture product includes the following steps. A predetermined quantity of mesenchymal stem cells is seeded in a flat culture device containing a first cell culture medium. When the mesenchymal stem cells proliferate to a target quantity, the first cell culture medium is replaced with a second cell culture medium, and the second cell culture medium is removed after incubation for 18 to 30 hours. A target cell culture medium is added and incubated for 48 to 72 hours. The target cell culture medium is collected repeatedly. The collected target cell culture medium is filtered and concentrated to obtain the mesenchymal stem cell culture product.