Abstract: The invention provides a method for Columbidae gender identification including: providing a DNA sample of a bird belonging to the Columbidae family; performing a polymerase chain reaction to the DNA sample with a first primer pair of a first primer designed within the region of the SEQ ID. NO.: 9 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof, and a second primer pair of a second primer designed within the region of the SEQ ID NO.: 10 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof; and determining gender by performing a melting curve analysis to a product from the polymerase chain reaction, wherein the result showing two peaks indicate a female gender and showing one peak indicate a male gender.

Abstract: The invention provides a free radical-induced intracellular DNA damage reducing reagent, including: an effective amount of a Gracilaria sp. extract, serving as an active ingredient, wherein the free radical-induced intracellular DNA damage reducing reagent is capable of reducing free radical-induced intracellular DNA damage. The invention also provides a free radical-induced intracellular DNA damage reducing medicament or health food and a free radical-induced intracellular DNA damage reducing skin care product or cosmetic.

Abstract: A method for gender identification of eagles includes: providing a DNA of an eagle; performing a probe-based real-time PCR using the DNA as a template, a universal primer pair P2/P8 as a primer pair and a first probe and a second probe as probes, wherein the 5? ends of the first probe and the second probe are labeled with a first fluorescent dye and a second fluorescent dye, respectively, and the first probe is a sequence with about 15-38 nucleotides in length of SEQ ID No. 1 and the second probe is a sequence with about 15-44 nucleotides in length of SEQ ID No. 2; and analyzing a result of the PCR, wherein if the result is positive for both the first and the second fluorescent dye, the eagle is a female, and if the result is positive for only the first fluorescent dye, the eagle is a male.

Abstract: The invention provides a method for Columbidae gender identification including: providing a DNA sample of a bird belonging to the Columbidae family; performing a polymerase chain reaction to the DNA sample with a first primer pair of a first primer designed within the region of the SEQ ID. NO.: 9 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof, and a second primer pair of a second primer designed within the region of the SEQ ID NO.: 10 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof; and determining gender by performing a melting curve analysis to a product from the polymerase chain reaction, wherein the result showing two peaks indicate a female gender and showing one peak indicate a male gender.

Abstract: A method for gender identification of eagles includes: providing a DNA of an eagle; performing a probe-based real-time PCR using the DNA as a template, a universal primer pair P2/P8 as a primer pair and a first probe and a second probe as probes, wherein the 5? ends of the first probe and the second probe are labeled with a first fluorescent dye and a second fluorescent dye, respectively, and the first probe is a sequence with about 15-38 nucleotides in length of SEQ ID No. 1 and the second probe is a sequence with about 15-44 nucleotides in length of SEQ ID No. 2; and analyzing a result of the PCR, wherein if the result is positive for both the first and the second fluorescent dye, the eagle is a female, and if the result is positive for only the first fluorescent dye, the eagle is a male.