Abstract: The disclosure relates to a fructosamine deglycase vector, a transgenic cell line and a genetic engineering bacteria expressing fructosamine deglycase, and use of fructosamine deglycase, and belongs to the technical field of production by a biological enzyme method. The disclosure provides a method for catalyzing the transfer of amino from an amino donor compound to an amino receptor compound by using an enzyme (fructosamine deglycase), or an expression vector or cloning vector expressing the enzyme, or a transgenic cell line and a genetic engineering bacteria expressing the enzyme. The method provided by the disclosure has sufficient substrate sources, which is not limited by raw materials, has mild preparing conditions, little environmental pollution, high reaction specificity, less impurities, which is easy for downstream separation and purification, and has low production cost.