Abstract: The invention relates to a double-microenvironment three-layer bionic hydrogel scaffold and a preparation method and application thereof, which are characterized in that includes a cartilage layer GL-HPKGN, an intermediate layer GL-GMA, and a bone layer GL-HP/GMAAT; by an enzymatic crosslinking reaction based on hydroxyphenylpropionic acid (HPA), kartogenin (KGN) is grafted onto gelatin to form a cartilage-specific microenvironment in GL-HPKGN; Based on hydroxyphenylpropionic acid (HPA), a dual crosslinking network was formed by enzyme crosslinking reaction and methacryloyl-glycidyl dimethicone (GMA) photo-crosslinking reaction, which was used to graft atorvastatin (AT) onto gelatin, forming GL-HP/GMAAT with bone-specific microenvironment; the intermediate layer GL-GMA was beneficial for forming a clear and defined cartilage-bone integrated structure. The introduction of the tide-line intermediate layer GL-GMA facilitated the formation of well-defined chondro-bone integrated structures.

Abstract: A core-shell polymer comprises a core portion and a shell portion that at least partially covers the core portion. The core portion comprises a structural unit derived from a monomer represented by Formula I, and the shell portion comprises a structural unit derived from a monomer represented by Formula I, a structural unit derived from a monomer represented by Formula II, and a structural unit derived from a monomer represented by Formula III, wherein R1, R2 and R3 are each independently selected from hydrogen, fluorine, chlorine or C1-3 alkyl containing at least one fluorine atom, R4, R5, R6, R7, R8, R9 are each independently selected from hydrogen or substituted or unsubstituted C1-3 alkyl, and Ar is substituted or unsubstituted aryl.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: The present disclosure provides for a method of detecting the displacement of a specific analyte, including providing an indicator in an organic phase to form a first composition; providing an aqueous phase with a test analyte to form a second composition; adding at least one organic solute to the organic phase; putting the first composition and second composition in contact with each other, thereby forming a lamellar phase and an interface between the first composition and second composition; and detecting the presence of the indicator in the organic phase, wherein the presence of the indicator in the organic phase indicates the presence of the specific analyte. The present disclosure also provides for a method of developing a high throughput assay for contemporaneous detection of multiple analytes. Further, the present disclosure provides for a biosensor for detection of a specific analyte.

Abstract: Senolytic and anti-inflammatory prodrugs are provided, which are designed from a cytotoxic agent, by chemically modifying the cytotoxic agent to incorporate a site cleavable by SA-?-gal following delivery of the prodrug in vivo, to release the active parent drug. The prodrug includes a galactose-based moiety, which is preferably acetylated, and benzyl oxy carboxy group and a cytotoxic agent moiety. The selenolytic prodrug is used to selectively kill one or more senescent cells and/or reduce an acute or chronic inflammatory response in a subject in need thereof, by administering to the subject therapeutically effective amount of the senolytic prodrugs. The disclosed compositions can be used to reduce one or more symptoms associated with a Senescence-associated disease or disorder or an inflammatory disorder, for example, a virus-mediated inflammation, in a subject.

Abstract: An optical fiber including a light guiding inner core. The light guiding inner core includes a first light guiding segment and a second light guiding segment connected to the first light guiding segment. The first light guiding segment includes, from the inside out, a light absorbing layer, an inner electrode layer, an insulating layer, a void layer, a proton exchange membrane, and an outer electrode layer. The void layer is formed between the insulating layer and the proton exchange membrane. The light absorbing layer is a photovoltaic material layer. The inner electrode layer communicates with the proton exchange membrane via a plurality of microelectrodes across the insulating layer and the void layer. The plurality of microelectrodes is evenly disposed around the inner electrode layer. The outer electrode layer is a porous conductive structure. The second light guiding segment of the light guiding inner core includes a conductive layer.

Abstract: A method for preparing an anode material for lithium-ion batteries, the method including: (1) mixing a raw residue from a biomass gasifier and an aqueous solution including a surfactant to yield a mixed solution, grinding the mixed solution to disperse the raw residue, washing the mixed solution using water to remove the surfactant, leaching the mixed solution, to yield a first intermediate residue; (2) adding hydrochloric acid to the first intermediate residue, stirring to remove impurities, filtering, and washing the residue to be neutral, to yield a second intermediate residue; (3) adding polyethyleneimine and ethanol to the second intermediate residue, shaking, washing away the polyethyleneimine and the ethanol, filtering, to yield a third intermediate residue; and (4) adding nitric acid to the third intermediate residue, fully stirring, washing away the nitric acid, filtering and drying, to yield the anode material for lithium-ion batteries.