Abstract: Disclosed are a CRISPR-Cas14a responsive photoelectrochemical sensing detection method and a kit for detecting T2 toxin, relating to the technical field of small molecule toxin detection; the detection method includes: (1) preparation of a magnetic probe; (2) electrode modification; (3) identification of magnetic beads; (4) SDA isothermal amplification; (5) cutting; (6) photoelectrochemical detection; and (7) standard curve plotting. According to the present application, using SDA to enlarge and amplify the signal isothermally, target is detected by Cas14a trans cleavage property and photoelectric signal with good stability, high specificity of reaction, high sensitivity.

Abstract: The present application relates to the technical field of wastewater treatment and rapid pollutant detection, in particular to a chitosan-polyacrylamide composite porous hydrogel, preparation and use thereof, and a metal ion-adsorbing and detecting reagent and method. The chitosan-polyacrylamide composite porous hydrogel of the present application is prepared by in situ polymerization of a chitosan sol, an acrylamide, a crosslinking agent and a surfactant into a mixed solution comprising liquid droplets, followed by steps of curing, washing, and freeze-drying. The present application further provides a metal ion-detecting reagent, which is obtained by adsorbing a color developing agent into the chitosan-polyacrylamide composite porous hydrogel as described above, wherein the color developing agent is a dye that changes color when encountering metal ions. The chitosan-polyacrylamide composite porous hydrogel of the present application has balanced mechanical properties and porosity.