Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: In some aspects, cardiac-specific expression cassettes are provided herein. In some aspects, provided herein is an expression cassette comprising a polynucleotide sequence encoding a gene product for therapy of a heart disease, wherein the polynucleotide sequence is operably linked to promoter (e.g., a cardiac-specific promoter), and optionally an enhancer (e.g., a cardiac-specific enhancer). In some aspects, the disclosure provides recombinant adeno-associated virus (rAAV) virions, comprising a capsid protein and a viral genome comprising an expression cassette comprising a polynucleotide sequence encoding a therapeutic gene product, e.g., dwarf open reading frame (DWORF) polypeptide, operably linked to a promoter, the expression cassette flanked by inverted terminal repeats. The disclosure further provides pharmaceutical compositions and methods of treating or preventing heart disease.

Abstract: A method for processing a sematic description of a text entity is proposed. The method includes: acquiring a plurality of target texts containing a main entity, and extracting related entities describing the main entity from each target text; acquiring a sub-relation vector of a pair of the main entity and each related entity in each target text; calculating a similarity distance of the main entity between different target texts based on the sub-relation vector; and determining a semantic similarity of the main entity descripted in different target texts based on the similarity distance.

Abstract: In some aspects, cardiac-specific expression cassettes are provided herein. In some aspects, provided herein is an expression cassette comprising a polynucleotide sequence encoding a gene product for therapy of a heart disease, wherein the polynucleotide sequence is operably linked to promoter (e.g., a cardiac-specific promoter), and optionally an enhancer (e.g., a cardiac-specific enhancer). In some aspects, the disclosure provides recombinant adeno-associated virus (rAAV) virions, comprising a capsid protein and a viral genome comprising an expression cassette comprising a polynucleotide sequence encoding a therapeutic gene product, e.g., dwarf open reading frame (DWORF) polypeptide, operably linked to a promoter, the expression cassette flanked by inverted terminal repeats. The disclosure further provides pharmaceutical compositions and methods of treating or preventing heart disease.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes by expression of selected microRNAs with MYOCD and ASCL1, or with MYOCD alone. Illustrative microRNAs include miR-133, miR-1, miR-19, and/or miR-20b. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides encoding a selected microRNA with YOCD, withMYOCD and ASCL1, with MYOCD-2A-ASCL1, or with ASCL1-2A-MYOCD. It further provides methods of using such compositions and vectors, or induced cardiomyocytes generated with these factors, for treating a heart condition.

Abstract: Embodiments of the present disclosure provide a method, apparatus, computer device, and storage medium for verifying community question answer data. The method may include: acquiring a community question answer data set, and generating a plurality of question answer pairs based on the community question answer data set, a question answer pair including: a question, and a to-be-verified answer corresponding to the question; generating an authoritative data set based on data stored in at least one confidence source site; and performing an authority verification on the to-be-verified answer, based on a score of a similarity between the to-be-verified answer and authoritative data in the authoritative data set in at least one dimension.

Abstract: A method for obtaining an answer to a question is provided. The method may include: acquiring a question; determining at least a part of articles in a preset article database as candidate articles, and determining first scores of the candidate articles respectively, the first score of any of the candidate articles representing a matching degree between the candidate article and the question; determining at least a part of texts in each of the candidate articles as candidate texts, and determining second scores of the candidate texts respectively, the second score of any of the candidate texts representing a matching degree between the candidate text and the question; and determining at least a part of the candidate texts as the answer based on a score set of each of the candidate texts, the score set of any of the candidate texts including the second score and the first score.

Abstract: Embodiments of the present disclosure provide a query processing method and an apparatus, a server and a storage medium. The method includes: determining a word vector representation of a query sequence and an entity vector representation of the query sequence respectively based on respective words and respective entities included in the query sequence; determining a word vector representation of a paragraph and an entity vector representation of the paragraph respectively based on respective words and respective entities included in the paragraph; and determining a similarity between the query sequence and the paragraph according to the word vector representation of the query sequence, the entity vector representation of the query sequence, the word vector representation of the paragraph, and the entity vector representation of the paragraph.

Abstract: The present disclosure discloses a method, apparatus, device, and storage medium for matching semantics, relates to the technical fields of knowledge graph, natural language processing, and deep learning. The method may include: acquiring a first text and a second text; acquiring language knowledge related to the first text and the second text; determining a target embedding vector based on the first text, the second text, and the language knowledge; and determining a semantic matching result of the first text and the second text, based on the target embedding vector.

Abstract: The present disclosure discloses a method, apparatus, device, and storage medium for training a model, relates to the technical fields of knowledge graph, natural language processing, and deep learning. The method may include: acquiring a first annotation data set, the first annotation data set including sample data and a annotation classification result corresponding to the sample data; training a preset initial classification model based on the first annotation data set to obtain an intermediate model; performing prediction on the sample data in the first annotation data set using the intermediate model to obtain a prediction classification result corresponding to the sample data; generating a second annotation data set based on the sample data, the corresponding annotation classification result, and the corresponding prediction classification result; and training the intermediate model based on the second annotation data set to obtain a classification model.

Abstract: The present disclosure discloses a question analysis method, a device, a knowledge base question answering system and an electronic equipment. The method includes: analyzing a question to obtain N linearized sequences, N being an integer greater than 1; converting the N linearized sequences into N network topology maps; separately calculating a semantic matching degree of each of the N network topology maps to the question; and selecting a network topology map having a highest semantic matching degree to the question as a query graph of the question from the N network topology maps. According to the technology of the present disclosure, the query graph of the question can be obtained more accurately, and the accuracy of the question to the query graph is improved, thereby improving the accuracy of question analysis.

Abstract: A method for processing a sematic description of a text entity is proposed. The method includes: acquiring a plurality of target texts containing a main entity, and extracting related entities describing the main entity from each target text; acquiring a sub-relation vector of a pair of the main entity and each related entity in each target text; calculating a similarity distance of the main entity between different target texts based on the sub-relation vector; and determining a semantic similarity of the main entity descripted in different target texts based on the similarity distance.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: The disclosure discloses a semantics processing method, a semantics processing apparatus, an electronic device, and a medium, and relates to a field of knowledge graph technologies. The detailed implementation includes: determining a target semantic element rule matching a text to be parsed, and parsing the text to be parsed by employing the target semantic element rule to obtain a semantic element parsing result; generating a semantic tree based on the semantic element parsing result by employing a target structured rule associated with the target semantic element rule; and performing semantic understanding on the text to be parsed based on the semantic tree.

Abstract: The present application discloses a method, an apparatus, a device, and a computer readable storage medium for determining a target content, the method includes: splitting an article paragraph determined according to search information into multiple sentences, and determining a relationship between the sentences according to attributes of the sentences; determining a sentence representation corresponding to each of the sentences according to the relationship between the sentences; and determining a target sentence according to the sentence representation of the sentence and the search information, and determining a target content according to the target sentence, so that the method, the apparatus, the device, and the computer readable storage medium provided by the present disclosure can analyze each of the sentences in combination with the relationship between the sentences, thereby determining a target content that more closely matches the search information.

Abstract: The present disclosure involves the use of reprogramming factors including AKT1, GATA4, TBX5, MEF2C, HAND2 and either ZNF281 or AS-CL1 to reprogram adult non-cardiomyocytes, such as cardiac fibroblasts into cardiomyocytes, both in vitro and in vivo. Such methods find particular use in the treatment of patients post-myocardial infarction to prevent or limit scarring and to promote myocardial repair.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: A method for obtaining an answer to a question is provided. The method may include: acquiring a question; determining at least a part of articles in a preset article database as candidate articles, and determining first scores of the candidate articles respectively, the first score of any of the candidate articles representing a matching degree between the candidate article and the question; determining at least a part of texts in each of the candidate articles as candidate texts, and determining second scores of the candidate texts respectively, the second score of any of the candidate texts representing a matching degree between the candidate text and the question; and determining at least a part of the candidate texts as the answer based on a score set of each of the candidate texts, the score set of any of the candidate texts including the second score and the first score.