Abstract: The present disclosure discloses a resource preheating method, system, apparatus and medium based on a content delivery network, wherein the content delivery network comprises a plurality of edge nodes for resource caching; the method comprises: creating an IP acquisition request based on a domain name that requires resource preheating and a first IP address of a client in a preheating area, so as to acquire a second IP address of a first edge node that the client needs to connect to; sending an access request to the first edge node to determine whether the first edge node has cached resources under the domain name according to the second IP address; and in response that the first edge node has not cached the resources under the domain name, preheating the resources under the domain name at the first edge node.

Abstract: Disclosed are a hinge and a refrigeration equipment. The hinge includes a vertical hinge shaft, a first hinge base and a second hinge base. The first hinge base includes a first installation plate and a first sleeve rotatably connected to the hinge shaft to make the first sleeve rotate around the hinge shaft. A lower end of the first sleeve is provided with a first slope sloping downwards. The second hinge base below the first hinge base includes a second installation plate and a second sleeve fixed on the hinge shaft. An upper end of the second sleeve is provided with a second slope sloping upwards. The second slope abuts against and cooperates with the first slope for the first sleeve to move from a first position to a second position along a direction away from the second sleeve or from the second position to the first position along a direction close to the second sleeve while being rotated around the hinge shaft.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: The present disclosure relates to the technical field of automated analysis, and provides a sample analysis system and a control method for the same and a sample analysis method. The sample analysis system includes: a control device; a rail device for carrying cuvettes under control of the control device; at least one independent sample distribution node arranged on the rail device for distributing samples to the cuvettes, the independent sample distribution node being used for sample aspiration, transfer, and discharge; and at least two independent reagent distribution and measurement nodes arranged on the rail device for distributing test reagents to the cuvettes and measuring the mixture liquid in the cuvettes, the independent reagent distribution and measurement nodes being used for reagent aspiration, transfer, and discharge and reagent-sample mixture liquid measurement.

Abstract: This application discloses a method of displaying information in a program interface of an application performed by a computer device. The method includes: displaying a virtual keyboard control and an extension bar control in the program interface; in response to an input operation in the virtual keyboard control, displaying at least one character string in the extension bar control, the at least one character string being determined according to the input operation in the virtual keyboard control; and in response to a select operation on a target string among the at least one character string in the extension bar control, displaying a function interface of applying a target function to the target string. This embodiment allows a user to quickly switch between function interfaces when using an application, thereby reducing operation steps of the user and improving human-computer interaction efficiency.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: Disclosed in the present invention is a high-strength double-sided stainless steel clad sheet, including a substrate layer, and stainless steel clad layers clad-rolled on double sides of the substrate layer. The substrate layer consists, in mass percent, the chemical elements: C: 0.03-0.12%, 0<Si?0.30%, Mn: 0.2-1.0%, Al: 0.02-0.04%, Ti: 0.01-0.03%, Nb: 0.005-0.020%, and N: 0.003-0.006%, with the balance being iron and other inevitable impurities. Also disclosed in the present invention is a manufacturing method for the high-strength double-sided stainless steel clad sheet, including the steps of: (1) obtaining the substrate layer and the stainless steel clad layer; (2) billet making; (3) clad-rolling; and (4) solution annealing and pickling treatment: controlling the solution temperature to be 950-1020° C., and then cooling down to room temperature at an average cooling rate of 20-50° C./s.

Abstract: A method for determining a protein structure using cryo-electron microscopy, including: enabling a target protein to contain a tag; enabling a resulting target containing the tag to bind a scaffold protein to form a complex between the target protein and the scaffold protein; and performing single-particle imaging using the cryo-electron microscopy to determine a structure of the target protein in complex with the scaffold protein. The scaffold protein is any one of streptavidin, avidin, or derivatives thereof. The tag is configured for selectively binding to the scaffold protein. The tag is one selected from the group consisting of: a biotin tag, comprising a biotin; a biotinylated protein or polypeptide tag, comprising a protein sequence and a biotin covalently linked to the protein sequence; a Strep-tag; and a biotinylated or strep-tagged antibody, or antibody Fab fragment, or single-chain antibody.

Abstract: The present invention belongs to the technical field of an object driving device, in particular to an object's motion attitude adjusting device and a puncturing, liquid filling and mixing system. The object's motion attitude adjusting apparatus includes a turntable, a plurality of object's motion attitude adjusting devices provided on the turntable, the object's motion attitude adjusting device including a base and a plurality of object's motion attitude adjusting mechanisms provided on the base, a first driving wheel connected with each of the object's motion attitude adjusting mechanisms in a transmission manner, a first driving component connected with the turntable for driving the turntable, and a second driving component connected with the first driving wheel for driving the first driving wheel.

Abstract: A method of modifying an upstream open reading frame (uORF) by genome editing technique to regulate gene expression.

Abstract: Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.

Abstract: Disclosed are a hinge and a refrigeration equipment. The hinge includes a vertical hinge shaft, a first hinge base and a second hinge base. The first hinge base includes a first installation plate and a first sleeve rotatably connected to the hinge shaft to make the first sleeve rotate around the hinge shaft. A lower end of the first sleeve is provided with a first slope sloping downwards. The second hinge base below the first hinge base includes a second installation plate and a second sleeve fixed on the hinge shaft. An upper end of the second sleeve is provided with a second slope sloping upwards. The second slope abuts against and cooperates with the first slope for the first sleeve to move from a first position to a second position along a direction away from the second sleeve or from the second position to the first position along a direction close to the second sleeve while being rotated around the hinge shaft.

Abstract: This application discloses a method of displaying information in a program interface of an application performed by a computer device. The method includes: displaying a virtual keyboard control and an extension bar control in the program interface; in response to an input operation in the virtual keyboard control, displaying at least one character string in the extension bar control, the at least one character string being determined according to the input operation in the virtual keyboard control; and in response to a select operation on a target string among the at least one character string in the extension bar control, displaying a function interface of applying a target function to the target string. This embodiment allows a user to quickly switch between function interfaces when using an application, thereby reducing operation steps of the user and improving human-computer interaction efficiency.

Abstract: The disclosure discloses a catalyst capable of simultaneously removing COS and H2S in garbage gasification and a preparation method thereof, and belongs to the technical field of preparation of desulfurization catalysts. The method includes the following steps: pretreating an SBA-15 molecular sieve with a templating agent unremoved, which primarily includes the steps of removing the templating agent and introducing halogen atoms to modify the molecular sieve; then synthesizing an active component solution; and finally introducing active components into channels of the pretreated molecular sieve via surface tension by adopting an impregnation method, performing washing and drying, and performing calcining under an N2 atmosphere, so as to obtain the catalyst. The catalyst prepared according to the present disclosure can load the active components in fixed positions inside and outside the channels, and the components are easy to obtain, thereby having the advantages of low cost and good desulfurization effects.

Abstract: The disclosure discloses a catalyst capable of simultaneously removing COS and H2S in garbage gasification and a preparation method thereof, and belongs to the technical field of preparation of desulfurization catalysts. The method includes the following steps: pretreating an SBA-15 molecular sieve with a templating agent unremoved, which primarily includes the steps of removing the templating agent and introducing halogen atoms to modify the molecular sieve; then synthesizing an active component solution; and finally introducing active components into channels of the pretreated molecular sieve via surface tension by adopting an impregnation method, performing washing and drying, and performing calcining under an N2 atmosphere, so as to obtain the catalyst.

Abstract: The present invention discloses an application of norharmane in improving activity of an antibiotic in resisting bacteria, when norharmane of the present invention is combined with polymyxin B, imipenem-cilastatin sodium or levofloxacin, it can enhance antibacterial activity of the antibiotic against drug-resistant Pseudomonas aeruginosa, produce a synergistic antibacterial effect, and significantly improves the killing ability of antibiotics against drug-resistant Pseudomonas aeruginosa to reduce the amount of antibiotics needed for achieving the same therapeutic effect, thereby providing a research direction for the development of new drugs and new use of old drugs.

Abstract: A refrigerator box body includes mounting bases (1) used to mount hinges, a box housing (2) provided with an upward opening, and an opening frame (3) connected to the opening of the box housing (2), a side plate of the box housing (2) being provided with mounting ports (4) penetrating the side plate of the box housing (2) along the thickness direction, the mounting bases (1) being arranged within the mounting ports (4), the mounting bases (1) being provided with depressions (11) with concave sides toward an inside of the box housing (2), the hinges being mounted within the depressions (11), the mounting bases (1) being provided with guide grooves (12), side edges of the mounting ports (4) being inserted into the guide grooves (12) so as to connect the mounting bases (1) and the box housing (2), an edge of the opening frame (3) abutting against upper sides of the mounting bases (1).

Abstract: Provided is a gene editing system for editing a target sequence in the genome of a cell, comprising a CRISPR nuclease, a cytosine deaminase, an AP lyase, a guide RNA and optionally an uracil-DNA glycosylase. Also provided are a method of producing a genetically modified cell, and a kit comprising the gene editing system.

Abstract: An image search method is provided. In the method, an image selection page of a messaging application is displayed. An input image for a search is determined based on an operation performed by a user on the image selection page. A search is performed for at least one output image similar to the input image based on a plurality of types of image characteristics that indicate levels of similarity between images. Each of the ay least one output image is determined to be similar to the input image based on at least one of the types of image characteristics. A search result list is generated according to the at least one output image. Further, the search result list is displayed.

Abstract: A refrigerator box body includes mounting bases (1) used to mount hinges, a box housing (2) provided with an upward opening, and an opening frame (3) connected to the opening of the box housing (2), a side plate of the box housing (2) being provided with mounting ports (4) penetrating the side plate of the box housing (2) along the thickness direction, the mounting bases (1) being arranged within the mounting ports (4), the mounting bases (1) being provided with depressions (11) with concave sides toward an inside of the box housing (2), the hinges being mounted within the depressions (11), the mounting bases (1) being provided with guide grooves (12), side edges of the mounting ports (4) being inserted into the guide grooves (12) so as to connect the mounting bases (1) and the box housing (2), an edge of the opening frame (3) abutting against upper sides of the mounting bases (1).