Abstract: The present invention relates to cyclopropane skeleton monophosphine ligands and preparation methods for palladium complexes thereof and an application. Specifically, trans-diaryl substituted cyclopropane skeleton monophosphine ligands are synthesized after trans-1,2-diaryl ethylene undergoing the steps of cyclopropanation reaction, debromination reaction, substitution reaction, and the like. Palladium complexes prepared by coordinating the cyclopropane skeleton monophosphine ligands with a palladium salt show very high activity in catalyzing C—N bond coupling reactions, and have good application prospects.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: A method for recovering C2 components in a methane-containing industrial gas includes the steps of (1) cooling a compressed methane-containing industrial gas and performing gas-liquid separation; (2) absorbing C2 components in the gas phase by using an absorbent to obtain an absorption rich liquid; (3) returning the absorption rich liquid to the compression in step (1) or mixing the absorption rich liquid with the liquid phase obtained in step (1) to obtain a mixed liquid, and depressurizing the mixed liquid or the absorption rich liquid; (4) performing methane desorption on the depressurized stream to obtain a rich absorbent, or performing second gas-liquid separation on the depressurized stream, followed by methane desorption on the second liquid phase to obtain a rich absorbent; and (5) desorbing and separating the rich absorbent to obtain a lean absorbent and an enriched gas, and recycling and reusing the lean absorbent.

Abstract: An electrode includes a current collector; a first active material layer including a first active material; and a second active material layer including a second active material; wherein the first active material layer is arranged between the current collector and the second active material layer. The first active material layer is formed on a surface of the current collector, and a particle size of 90% accumulative volume of the first active material is less than 40 ?m. The active material layer is used to ensure that the electrochemical device and the electronic device do not generate a short circuit when pressed by an external force, thereby ensuring the mechanical safety performance of the electrochemical device and the electronic device.

Abstract: A data prefetching method includes a computing node obtaining information about accessing a storage node by a first application in a preset time period. The computing node determines information about prefetch data based on the access information. The computing node determines, based on the information about the prefetch data, a cache node prefetching the prefetch data, and generates a prefetch request for prefetching the prefetch data. The computing node sends the prefetch request to the cache node. The cache node performs a prefetching operation on the prefetch data in response to the prefetch request.

Abstract: This application provides a water conservancy project operation trend prediction method. The method includes: acquiring to-be-processed initial monitoring data, and determining a first type of monitoring data and a second type of monitoring data according to the initial monitoring data; determining a target nonlinear fitting model from a preset nonlinear fitting model set according to the second type of monitoring data; and predicting the state of a target water conservancy project by a new model constructed by combining the target nonlinear fitting model with the models in the preset prediction model set.

Abstract: The present disclosure relates to the technical field of automated analysis, and provides a sample analysis system and a control method for the same and a sample analysis method. The sample analysis system includes: a control device; a rail device for carrying cuvettes under control of the control device; at least one independent sample distribution node arranged on the rail device for distributing samples to the cuvettes, the independent sample distribution node being used for sample aspiration, transfer, and discharge; and at least two independent reagent distribution and measurement nodes arranged on the rail device for distributing test reagents to the cuvettes and measuring the mixture liquid in the cuvettes, the independent reagent distribution and measurement nodes being used for reagent aspiration, transfer, and discharge and reagent-sample mixture liquid measurement.

Abstract: This application discloses a method of displaying information in a program interface of an application performed by a computer device. The method includes: displaying a virtual keyboard control and an extension bar control in the program interface; in response to an input operation in the virtual keyboard control, displaying at least one character string in the extension bar control, the at least one character string being determined according to the input operation in the virtual keyboard control; and in response to a select operation on a target string among the at least one character string in the extension bar control, displaying a function interface of applying a target function to the target string. This embodiment allows a user to quickly switch between function interfaces when using an application, thereby reducing operation steps of the user and improving human-computer interaction efficiency.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: Disclosed in the present invention is a high-strength double-sided stainless steel clad sheet, including a substrate layer, and stainless steel clad layers clad-rolled on double sides of the substrate layer. The substrate layer consists, in mass percent, the chemical elements: C: 0.03-0.12%, 0<Si?0.30%, Mn: 0.2-1.0%, Al: 0.02-0.04%, Ti: 0.01-0.03%, Nb: 0.005-0.020%, and N: 0.003-0.006%, with the balance being iron and other inevitable impurities. Also disclosed in the present invention is a manufacturing method for the high-strength double-sided stainless steel clad sheet, including the steps of: (1) obtaining the substrate layer and the stainless steel clad layer; (2) billet making; (3) clad-rolling; and (4) solution annealing and pickling treatment: controlling the solution temperature to be 950-1020° C., and then cooling down to room temperature at an average cooling rate of 20-50° C./s.

Abstract: A method for determining a protein structure using cryo-electron microscopy, including: enabling a target protein to contain a tag; enabling a resulting target containing the tag to bind a scaffold protein to form a complex between the target protein and the scaffold protein; and performing single-particle imaging using the cryo-electron microscopy to determine a structure of the target protein in complex with the scaffold protein. The scaffold protein is any one of streptavidin, avidin, or derivatives thereof. The tag is configured for selectively binding to the scaffold protein. The tag is one selected from the group consisting of: a biotin tag, comprising a biotin; a biotinylated protein or polypeptide tag, comprising a protein sequence and a biotin covalently linked to the protein sequence; a Strep-tag; and a biotinylated or strep-tagged antibody, or antibody Fab fragment, or single-chain antibody.

Abstract: The present invention belongs to the technical field of an object driving device, in particular to an object's motion attitude adjusting device and a puncturing, liquid filling and mixing system. The object's motion attitude adjusting apparatus includes a turntable, a plurality of object's motion attitude adjusting devices provided on the turntable, the object's motion attitude adjusting device including a base and a plurality of object's motion attitude adjusting mechanisms provided on the base, a first driving wheel connected with each of the object's motion attitude adjusting mechanisms in a transmission manner, a first driving component connected with the turntable for driving the turntable, and a second driving component connected with the first driving wheel for driving the first driving wheel.

Abstract: Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.

Abstract: A method of modifying an upstream open reading frame (uORF) by genome editing technique to regulate gene expression.

Abstract: Disclosed are a hinge and a refrigeration equipment. The hinge includes a vertical hinge shaft, a first hinge base and a second hinge base. The first hinge base includes a first installation plate and a first sleeve rotatably connected to the hinge shaft to make the first sleeve rotate around the hinge shaft. A lower end of the first sleeve is provided with a first slope sloping downwards. The second hinge base below the first hinge base includes a second installation plate and a second sleeve fixed on the hinge shaft. An upper end of the second sleeve is provided with a second slope sloping upwards. The second slope abuts against and cooperates with the first slope for the first sleeve to move from a first position to a second position along a direction away from the second sleeve or from the second position to the first position along a direction close to the second sleeve while being rotated around the hinge shaft.

Abstract: This application discloses a method of displaying information in a program interface of an application performed by a computer device. The method includes: displaying a virtual keyboard control and an extension bar control in the program interface; in response to an input operation in the virtual keyboard control, displaying at least one character string in the extension bar control, the at least one character string being determined according to the input operation in the virtual keyboard control; and in response to a select operation on a target string among the at least one character string in the extension bar control, displaying a function interface of applying a target function to the target string. This embodiment allows a user to quickly switch between function interfaces when using an application, thereby reducing operation steps of the user and improving human-computer interaction efficiency.

Abstract: The disclosure discloses a catalyst capable of simultaneously removing COS and H2S in garbage gasification and a preparation method thereof, and belongs to the technical field of preparation of desulfurization catalysts. The method includes the following steps: pretreating an SBA-15 molecular sieve with a templating agent unremoved, which primarily includes the steps of removing the templating agent and introducing halogen atoms to modify the molecular sieve; then synthesizing an active component solution; and finally introducing active components into channels of the pretreated molecular sieve via surface tension by adopting an impregnation method, performing washing and drying, and performing calcining under an N2 atmosphere, so as to obtain the catalyst. The catalyst prepared according to the present disclosure can load the active components in fixed positions inside and outside the channels, and the components are easy to obtain, thereby having the advantages of low cost and good desulfurization effects.

Abstract: The disclosure discloses a catalyst capable of simultaneously removing COS and H2S in garbage gasification and a preparation method thereof, and belongs to the technical field of preparation of desulfurization catalysts. The method includes the following steps: pretreating an SBA-15 molecular sieve with a templating agent unremoved, which primarily includes the steps of removing the templating agent and introducing halogen atoms to modify the molecular sieve; then synthesizing an active component solution; and finally introducing active components into channels of the pretreated molecular sieve via surface tension by adopting an impregnation method, performing washing and drying, and performing calcining under an N2 atmosphere, so as to obtain the catalyst.

Abstract: The present invention discloses an application of norharmane in improving activity of an antibiotic in resisting bacteria, when norharmane of the present invention is combined with polymyxin B, imipenem-cilastatin sodium or levofloxacin, it can enhance antibacterial activity of the antibiotic against drug-resistant Pseudomonas aeruginosa, produce a synergistic antibacterial effect, and significantly improves the killing ability of antibiotics against drug-resistant Pseudomonas aeruginosa to reduce the amount of antibiotics needed for achieving the same therapeutic effect, thereby providing a research direction for the development of new drugs and new use of old drugs.

Abstract: A refrigerator box body includes mounting bases (1) used to mount hinges, a box housing (2) provided with an upward opening, and an opening frame (3) connected to the opening of the box housing (2), a side plate of the box housing (2) being provided with mounting ports (4) penetrating the side plate of the box housing (2) along the thickness direction, the mounting bases (1) being arranged within the mounting ports (4), the mounting bases (1) being provided with depressions (11) with concave sides toward an inside of the box housing (2), the hinges being mounted within the depressions (11), the mounting bases (1) being provided with guide grooves (12), side edges of the mounting ports (4) being inserted into the guide grooves (12) so as to connect the mounting bases (1) and the box housing (2), an edge of the opening frame (3) abutting against upper sides of the mounting bases (1).