Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: The present invention discloses a method for detecting open phase of a startup/standby transformer based on optical CT. A startup/standby transformer in a power plant is in a no-load condition for a long time as a standby power supply. Once a single-phase open phase fault occurs, there is no significant change in the voltage phasor and voltage sequence component of each side. If not found in time, the defect may pose a great threat to the safe operation of the power plant. In the present invention, the optical CT is used to detect a three-phase current of the high-voltage side of the startup/standby transformer. If the current satisfies an open phase criterion, it is determined that an open phase fault occurs, and then, an alarm signal is given after a delay and an operator is informed to handle the fault in time. Therefore, the operation reliability of the startup/standby transformer system in the power plant is enhanced.

Abstract: The invention provides use of NW_006883358.1 in a CHO cell genome for stable expression of a protein. A site for stable expression of a protein in the CHO cell genome is located at the 6874389th base of CHO cell gene NW_006883358.1; 5?NNNNNNNNNNNNNNNNNNNNNGG3? within a range of 6874325-6874443 near the site, which can be identified by CRISPR/Cas9 technology, is a target sequence. In the present application, different protein genes are introduced to a fixed position in the CHO cell genome for stable expression.

Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: The present invention discloses a method for detecting open phase of a startup/standby transformer based on optical CT. A startup/standby transformer in a power plant is in a no-load condition for a long time as a standby power supply. Once a single-phase open phase fault occurs, there is no significant change in the voltage phasor and voltage sequence component of each side. If not found in time, the defect may pose a great threat to the safe operation of the power plant. In the present invention, the optical CT is used to detect a three-phase current of the high-voltage side of the startup/standby transformer. If the current satisfies an open phase criterion, it is determined that an open phase fault occurs, and then, an alarm signal is given after a delay and an operator is informed to handle the fault in time. Therefore, the operation reliability of the startup/standby transformer system in the power plant is enhanced.

Abstract: A method and apparatus for identifying an open phase of a circuit breaker on the basis of voltage. The method comprises: a protection apparatus measures three-phase voltages at two sides of a circuit breaker; respectively calculates a vector difference and an effective value of the voltages at two sides of the circuit breaker; when the phase voltages at two sides are both greater than 80% to 90% of a rated voltage, and the voltage vector difference at two sides of the one-phase or two-phase circuit breaker is greater than a set voltage, it is determined that the circuit breaker has an open phase; and actuates an alarming or tripping operation after a short time delay t.

Abstract: A relay protection method against LC parallel circuit detuning faults comprises the steps of: a relay protection device samples a current of a parallel LC, that is, a reactor and a capacitor, and samples a total current flowing through the whole LC; convert the current of the reactor into a current of an equivalent capacitor; calculate amplitudes of the current of the equivalent capacitor and a current of a realistic capacitor and calculate an amplitude of the total current flowing through the LC; calculate a current amplitude ratio of the equivalent capacitor to the realistic capacitor; and when the amplitude of the total current flowing through the LC is large enough, send an alarm signal or a trip after a setting time delay if the current ratio exceeds a preset upper and lower limit range. Also provided is a corresponding relay protection device.

Abstract: A method and apparatus for identifying an open phase of a circuit breaker on the basis of voltage. The method comprises: a protection apparatus measures three-phase voltages at two sides of a circuit breaker; respectively calculates a vector difference and an effective value of the voltages at two sides of the circuit breaker; when the phase voltages at two sides are both greater than 80% to 90% of a rated voltage, and the voltage vector difference at two sides of the one-phase or two-phase circuit breaker is greater than a set voltage, it is determined that the circuit breaker has an open phase; and actuates an alarming or tripping operation after a short time delay t.

Abstract: A frequency-conversion differential protection method for an output transformer of a static frequency convertor system.

Abstract: A relay protection method against LC parallel circuit detuning faults comprises the steps of: a relay protection device samples a current of a parallel LC, that is, a reactor and a capacitor, and samples a total current flowing through the whole LC; convert the current of the reactor into a current of an equivalent capacitor; calculate amplitudes of the current of the equivalent capacitor and a current of a realistic capacitor and calculate an amplitude of the total current flowing through the LC; calculate a current amplitude ratio of the equivalent capacitor to the realistic capacitor; and when the amplitude of the total current flowing through the LC is large enough, send an alarm signal or a trip after a setting time delay if the current ratio exceeds a preset upper and lower limit range. Also provided is a corresponding relay protection device.

Abstract: A frequency-conversion differential protection method for an output transformer of a static frequency convertor system.