Patents by Inventor Huey-Ming MAK

Huey-Ming MAK has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • INCREASING PRODUCTIVITY OF E. COLI HOST CELLS THAT FUNCTIONALLY EXPRESS P450 ENZYMES
    Publication number: 20230323314
    Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
    Type: Application
    Filed: January 31, 2023
    Publication date: October 12, 2023
    Inventors: Jason DONALD, Christopher PIRIE, Liwei LI, Huey-Ming MAK, Srishti TIBREWALA, Ajikumar PARAYIL KUMARAN
  • ENHANCED PRODUCTION OF HISTIDINE, PURINE PATHWAY METABOLITES, AND PLASMID DNA
    Publication number: 20230065419
    Abstract: Aspects of the disclosure relate to biosynthesis of histidine in host cells. For example, host cells may comprise: a promoter; a ribosome binding site (RBS); and a nucleic acid comprising: hisG; hisD; hisC hisB; hisH; hisA; hisF; and/or hisI. Host cells may further comprise a nucleic acid encoding a ribose phosphate pyrophosphokinase (RPPK), optionally comprising one or more amino acid substitutions relative to the sequence of wildtype E. coli RPPK. Host cells of the disclosure may comprise a nucleic acid encoding a 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase (MTHFDC) enzyme. Further aspects of the disclosure relate to production of purine pathway metabolites and/or plasmid DNA in host cells.
    Type: Application
    Filed: December 16, 2020
    Publication date: March 2, 2023
    Applicant: Ginkgo Bioworks, Inc.
    Inventors: Alkiviadis Orfefs Chatzivasileiou, Jason King, Huey-Ming Mak, Gabriel Rodriguez, Emily E. Wrenbeck, David Young
  • INCREASING PRODUCTIVITY OF E. COLI HOST CELLS THAT FUNCTIONALLY EXPRESS P450 ENZYMES
    Publication number: 20200377865
    Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
    Type: Application
    Filed: August 11, 2020
    Publication date: December 3, 2020
    Inventors: Jason DONALD, Christopher PIRIE, Liwei LI, Huey-Ming MAK, Srishti TIBREWALA, Ajikumar Parayil KUMARAN
  • Increasing productivity of host cells that functionally express P450 enzymes
    Patent number: 10774314
    Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: September 15, 2020
    Assignee: MANUS BIO INC.
    Inventors: Jason Donald, Christopher Pirie, Liwei Li, Huey-Ming Mak, Srishti Tibrewala, Ajikumar Parayil Kumaran
  • INCREASING PRODUCTIVITY OF E. COLI HOST CELLS THAT FUNCTIONALLY EXPRESS P450 ENZYMES
    Publication number: 20180251738
    Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
    Type: Application
    Filed: August 19, 2016
    Publication date: September 6, 2018
    Inventors: Jason DONALD, Christopher PIRIE, Liwei LI, Huey-Ming MAK, Srishti TIBREWALA, Ajikumar Parayil KUMARAN