Abstract: An enzyme immunoassay device comprises a disk having a thin flexible membrane applied to one side thereof, the membrane defining a conduit and a plurality of reagent reservoirs. The reservoirs are isolated from one another or from the conduit by frangible seals. An assay tube having an assay reagent bonded to the inner wall thereof is attached to the exit end of the conduit. Means are provided for injecting a test sample into the conduit for flowing thereof through the assay tube. Means are also provided for forcing the contents of each reservoir through the conduit and assay tube in the desired order by causing the rupture of each frangible seal sequentially.