Abstract: An identification system of circulating biomarkers for cancer detection, a development method of circulating biomarkers for cancer detection, a cancer detection method and a kit are provided in the present disclosure, and the development method includes the following steps. Expression levels of multiple genes in normal tissue samples and tumor tissue samples are identified, and genes with high expression levels in the tumor tissue samples are selected. Afterwards, a weight of each human tissue’s contribution to plasma exosomes is calculated using tissue-specific genes and group-enriched genes. Next, expression levels of plasma exosome genes of healthy people and cancer patients are compared by an overlapping index, and circulating biomarkers and combinations thereof suitable for detection and evaluation of plasma exosomes are selected.

Abstract: A biosensor and a biological detection method are provided. The biosensor includes a substrate, a plurality of first and second electrodes in a strip shape, a material layer and a capture antibody. The substrate has a sampling region and a measuring region connected to the sampling region. The first and second electrodes are disposed on the substrate in the measuring region, and the first and second electrodes are arranged alternately with each other, wherein the first electrodes are connected in parallel with each other, and the second electrodes are connected in parallel with each other.

Abstract: A biosensor and a biological detection method are provided. The biosensor includes a substrate, a plurality of first and second electrodes in a strip shape, a material layer and a capture antibody. The substrate has a sampling region and a measuring region connected to the sampling region. The first and second electrodes are disposed on the substrate in the measuring region, and the first and second electrodes are arranged alternately with each other, wherein the first electrodes are connected in parallel with each other, and the second electrodes are connected in parallel with each other.

Abstract: The present invention provides a method for detecting liver fibrotic injury, including fibrosis and/or cirrhosis, by assaying biological samples for differential expression of at least one gene encoding a protein chosen from SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof, wherein differential expression of at least one gene suggests the presence of liver fibrosis or cirrhosis. The invention also provides a kit containing nucleic acid probes or antibodies for detecting liver fibrosis and/or cirrhosis by assaying the differential expression of proteins encoded by SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof.

Abstract: The present invention provides a method for detecting liver fibrotic injury, including fibrosis and/or cirrhosis, by assaying biological samples for differential expression of at least one gene encoding a protein chosen from SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof, wherein differential expression of at least one gene suggests the presence of liver fibrosis or cirrhosis. The invention also provides a kit containing nucleic acid probes or antibodies for detecting liver fibrosis and/or cirrhosis by assaying the differential expression of proteins encoded by SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof.