Abstract: The application belongs to the technical field of cell processing, in particular to a cell processing device. The weighing device of the cell processing device is provided with at least one set of weighing components. These components allow one or more samples hung on the hook to be weighed and detected simultaneously, increasing weighing efficiency. The samples hung on each hook are weighed using the designated weighing sensor, so the weighing results won't interfere with one another. Moreover, each set of weighing components in this application can be disassembled and reassembled, making overhaul and maintenance easier.

Abstract: The disclosure relates to a re-writable DNA-based digital storage system with a random access feature. An example embodiment includes reading a physical nucleotide sequence of 2n+L bases to form a digital representation of the physical nucleotide sequence; dividing the digital representation of the physical nucleotide sequence into an address representation of n bases, followed by a data representation of L bases, followed by a further address representation of n bases; decoding the data representation into an integer value less than 3L that is a sum of a first addend and a second addend, wherein the data representation includes a first subsequence of bases encoding the first addend followed by a second subsequence of bases encoding the second addend; and storing, in a computer memory, the integer value.

Abstract: Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.

Abstract: The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system. Provided herein are methods and compositions for improvement of Cas12a on-target editing efficiency by incorporating 2-aminoadenine (base Z, which alters canonical Watson-Crick base pairing) into the crRNA to increase the binding affinity between crRNA and its complementary DNA target. The resulting CRISPR-Cas12a (named zCRISPR-Cas12a thereafter) shows an on-target editing efficiency comparable to that of the CRISPR-Cas9 system but with much lower off-target effects than the CRISPR-Cas9 system in mammalian cells. In addition, zCRISPR-Cas12a can be used for precise gene knock-in and highly efficient multiplex genome editing. Overall, the zCRISPR-Cas12a systems are superior to the CRISPR-Cas9 system.

Abstract: The present disclosure provides polynucleotides and vectors for the genomic engineering and editing of non-conventional yeasts such as Issatchenkia orientalis. The polynucleotides and vectors can be used as tools that are efficient to alter the expression of one or more gene products in the yeast, and specifically to induce the production of organic acids or other bioproducts of interest in the yeast.

Abstract: The present disclosure provides assay containers, sets of containers, portable diagnostic devices, methods of detecting target nucleic acid molecules in a sample, and methods of diagnosing infections, such as COVID-19 infections, in a subject.

Abstract: Provided herein are genetically modified Issatchenkia yeast and fermentation methods for producing succinic acid.

Abstract: Methods for removing sulfate from a battery electrode, methods for refurbishing batteries, methods of depositing a film on a battery electrode, and refurbished batteries are described. Methods for removing sulfate from a batter electrode include placing the battery electrode in a chelate solution to solubilize the sulfate and remove sulfate deposits from the battery electrode. Methods further include performing electrodeposition of a metal film on a battery electrode using chelate-metal solution resulting from the soaking process.

Abstract: Nick-based methods, devices, and systems for nick-based data storage in a deoxyribonucleic acid (DNA) sequence are disclosed. Digital information is encoded in a register of at least one copy of a double-stranded DNA sequence having a plurality of nickable positions. The data is translated into a sequence of values from a nick alphabet that is subsequently mapped to the plurality of nickable positions, and the DNA sequence is nicked according to the mapped values. Because the digital information is encoded as a series of nicked and non-nicked positions of a double-stranded DNA sequence, the nucleotide sequence of the DNA can be non-synthetic, or “native” DNA.

Abstract: Methods and compositions for specific cleavage of DNA molecules using single-stranded guides and Argonaute (Ago) proteins are described.

Abstract: Methods and compositions for specific cleavage of DNA molecules using single-stranded guides and Argonaute (Ago) proteins are described.

Abstract: The present disclosure provides polynucleotides and vectors for the genomic engineering and editing of non-conventional yeasts such as Issatchenkia orientalis. The polynucleotides and vectors can be used as tools that are efficient to alter the expression of one or more gene products in the yeast, and specifically to induce the production of organic acids or other bioproducts of interest in the yeast.

Abstract: Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.

Abstract: Methods and compositions for specific cleavage of DNA molecules using single-stranded guides and Argonaute (Ago) proteins are described.

Abstract: The disclosure relates to a re-writable DNA-based digital storage system with a random access feature. An example embodiment includes selecting address representations of m nucleotide sequences of n bases each. Each of the address representations of m nucleotide sequences (i) consists of approximately 50% guanine and cytosine content and (ii) is self-uncorrelated. The address representations are mutually uncorrelated with one another. All of the address representations end with a particular base. The embodiment also includes selecting, for a particular address representation from the address representations of the m nucleotide sequences, a corresponding data representation of a nucleotide sequence of L bases. The embodiment further includes concatenating the particular address representation with the data representation to form a representation of a target nucleotide sequence of n+L bases. In addition, the embodiment includes synthesizing the target nucleotide sequence.

Abstract: The disclosure relates to a re-writable DNA-based digital storage system with a random access feature. An example embodiment includes reading a physical nucleotide sequence of 2n+L bases to form a digital representation of the physical nucleotide sequence; dividing the digital representation of the physical nucleotide sequence into an address representation of n bases, followed by a data representation of L bases, followed by a further address representation of n bases; decoding the data representation into an integer value less than 3L that is a sum of a first addend and a second addend, wherein the data representation includes a first subsequence of bases encoding the first addend followed by a second subsequence of bases encoding the second addend; and storing, in a computer memory, the integer value.

Abstract: Systems and methods for automated workflow comprise assigning a set of first targets to an uncompiled first workflow. The uncompiled first workflow specifies a first set of process modules. Each such module is associated with a subset of unit operations. Each unit operation includes a time interval and specifies an instrument. For each target in the set of first targets, the uncompiled workflow is translated into an instance of a compiled first workflow comprising a linear temporal order of unit operations, each including execution instructions for an addressed instrument. A set of second targets is obtained and assigned a second uncompiled workflow. Compilation of the second uncompiled workflow for each second target produces a different instance of a compiled second workflow. Each second compiled workflow comprises a linear temporal order of unit operations, with each unit operation including execution instructions for an addressed instrument and specifying a time interval.

Abstract: Provided herein are methods and compositions for a CRISPR and homology-directed-repair assisted genome-scale engineering that can rapidly output tens of thousands of specific genetic variants in host cells. More than 98% of target sequences can be efficiently edited with a high average frequency.

Abstract: The present disclosure provides a combinatorial metabolic engineering system based on an orthogonal tri-functional CRISPR system that combines transcriptional activation, transcriptional interference, and gene deletion (CRISPR-AID). This strategy enables perturbation of the metabolic and regulatory networks in a modular, parallel, and high throughput manner. The present disclosure further provides a multi-functional genome-wide CRISPR (MAGIC) system for high throughput genotype-phenotype mapping.

Abstract: A gingival cone collar for dental implants includes a top section, the top section having a truncated zone shape and including a top open end and a bottom open end, the top open end being larger than the bottom open end; a connection section, the connection section having a ring shape; and a bottom section, the bottom section having a top open end and a bottom open end. The connection section connects the bottom open end of the top section with the top open end of the bottom section.