Abstract: The present disclosure relates to a method of forming a keratin filament network, comprising: (i) dialysing a solubilized keratin solution in a dialysis buffer solution at a pH of about 2.5 to about 5.5 to obtain purified keratin; (ii) mixing the purified keratin with a salt in the acidic buffer solution; and (ill) drying the solution of step (ii) to form the keratin filament network. In one embodiment, he dialysis buffer solution comprises a weak acid, a denaturing agent and a reducing agent. The said method comprises self-assembly of the keratin filament network. The present disclosure also relates to a keratin filament network comprising at least 2 Type I human hair keratins and at least 2 Type II human hair keratins.